Team:KULeuven/7 September 2008

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Another day, another lab report:

• We made a gel with the PCR samples of the ligation test and with the digests (K145201, K145205, K145253, K145251 and R0040+J23022+J23109+J23032). The result was very good, we now have a lot of new BioBricks that are finished. Only R0040+J23022+J23109+J23032 gave bad results. Both the PCR and the digest was too short. This is the second time that this ligations fails. We think the problem lies in the first part (R0040+J23022). We will not use this one anymore. We wil use R0040+J23066 instead. This ligation definitely succeeded and this part contains key3d and is compatible with the old lock (J23032).
• Yet another failure: PCR of T7 polymerase with UmuD tag failed again (surprise!).
• We also found out that the part R1052 doesn't contain a prefix or suffix. That's why the digest and PCR didn't work. We will make some oligos to construct it ourself.
• We electroporated the following parts: K145150+pSB1A2, K145150+E0240, K145150+B0034, K145013+pSB1A2, R0040+P0353, E0040+B0015, B0014+B0033+C0061+B0015 and K145254.
• Set up some ligations: K145201+K145205, K145201+(R0040+E0240), (R0040+B0033)+(C0056+B0015), (R0040+J23066)+(J23109+J23032), (J23109+J23032)+(K145001+B0015) and (J23116+B0032)+(C0062+B0015).

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