Team:KULeuven/9 September 2008

From 2008.igem.org

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== Lab Work ==
== Lab Work ==
=== Wet Lab ===
=== Wet Lab ===
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* We MiniPrepped the ligations (K145150+E0240, R0040+P0353, E0040+B0015, K145254, K145150+B0034, K145013+pSB1A2, C0062). Then we made digests of them.
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* The colonies resulting from the electroporation were streaked out. K145280 colonies were not fluorescent, K145279 were. Maybe this is because of the long lifetime of GFP.
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* We did a PCR to test the ligations. Only K145201+K145205, K145201+R0040+E0240, R0040+B0033+C0056+B0015 seemed fine. We made a liquid culture of them.
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* We made a glycerolstock of some successful ligations.
=== Dry Lab ===
=== Dry Lab ===
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==== Modeling ====
==== Modeling ====
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Finished the MultiCell Toolbox and edited its Wiki-page.
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Finished the MultiCell Toolbox and edited its [https://2008.igem.org/Team:KULeuven/Software/MultiCell Wiki-page].
==== Wiki ====
==== Wiki ====
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[[Image:Views.PNG|center]]
[[Image:Views.PNG|center]]
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Latest revision as of 19:36, 28 October 2008

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Contents

Lab Work

Wet Lab

  • We MiniPrepped the ligations (K145150+E0240, R0040+P0353, E0040+B0015, K145254, K145150+B0034, K145013+pSB1A2, C0062). Then we made digests of them.
  • The colonies resulting from the electroporation were streaked out. K145280 colonies were not fluorescent, K145279 were. Maybe this is because of the long lifetime of GFP.
  • We did a PCR to test the ligations. Only K145201+K145205, K145201+R0040+E0240, R0040+B0033+C0056+B0015 seemed fine. We made a liquid culture of them.
  • We made a glycerolstock of some successful ligations.

Dry Lab

Modeling

Finished the MultiCell Toolbox and edited its Wiki-page.

Wiki

Last edits were conducted to the picture gallery, so it doesn't look like... well you know what, no more. Wiki finished.

Remarks

We discovered on the iGEM - Statistics page that our website is ranked n°2 in the list of Most Viewed Pages (with a n°1 place for the iGEM home page).

Views.PNG