Team:KULeuven/Freezer
From 2008.igem.org
(Difference between revisions)
m (→Parts stored in the fridge in the lab (4°C)) |
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|[http://partsregistry.org/Part:BBa_C0012 C0012]||pSB1A2||LacI||+/- 30||133.1||21||July 29 | |[http://partsregistry.org/Part:BBa_C0012 C0012]||pSB1A2||LacI||+/- 30||133.1||21||July 29 | ||
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
- | |[http://partsregistry.org/Part:BBa_C0012 C0012]||pSB1A2||LacI repressor||+/- 40||146.4|| | + | |[http://partsregistry.org/Part:BBa_C0012 C0012]||pSB1A2||LacI repressor||+/- 40||146.4||64||July 29 |
|- style="background: #E0FFFF; text-align: center;" | |- style="background: #E0FFFF; text-align: center;" | ||
|[http://partsregistry.org/Part:BBa_C0012 C0012]||pSB1A2||LacI repressor||+/- 40||203.1||50||July 29 | |[http://partsregistry.org/Part:BBa_C0012 C0012]||pSB1A2||LacI repressor||+/- 40||203.1||50||July 29 |
Revision as of 09:53, 31 August 2008
dock/undock dropdown
Parts stored in the fridge in the lab (4°C)
MiniPrep Products: Parts |
---|
Part | Plasmid | Description | µl left | ng/µl | Box | Date |
---|---|---|---|---|---|---|
B0015 | pSB1AK3 | Terminator | +/- 10 | 112.3 | 11 | July 24 |
B0015 | pSB1AK3 | Terminator | +/- 20 | 130.3 | 12 | July 24 |
B0015 | pSB1AK3 | Terminator | +/- 43 | 303.1 | 13 | July 29 |
B0015 | pSB1AK3 | Terminator | +/- 20 | 213.6 | 14 | July 29 |
B0015 | pSB1AK3 | Terminator | +/- 48 | 311.0 | 31 | July 29 |
B0015 | pSB1AK3 | Terminator | +/- 30 | 214.0 | 47 | July 29 |
B0015 | pSB1AK3 | Terminator | +/- 40 | 209.5 | 48 | July 29 |
B0015 | pSB1AK3 | Terminator | +/- 40 | 63.0 | 10 | July 23 |
B0032 | pSB1A2 | RBS(medium) | +/- 5 | 51.0 | 56 | August 11 |
B0032 | pSB1A2 | RBS(medium) | +/- 20 | 47.2 | 57 | August 27 |
B0032 | pSB1A2 | RBS(medium) | +/- 40 | 27.0 | 58 | August 27 |
B0033 | pSB1AK3 | RBS | +/- 50 | 36.5 | 35 | August 27 |
B0033 | pSB1AK3 | RBS | +/- 23 | 38.4 | 41 | August 27 |
B0034 | pSB1A2 | RBS (efficiency 1) | +/- 20 | 73.8 | 5 | July 24 |
B0034 | pSB1A2 | RBS (efficiency 1) | +/- 30 | 95.1 | 6 | July 24 |
B0034 | pSB1A2 | RBS (efficiency 1) | +/- 40 | 104.0 | 7 | July 29 |
B0034 | pSB1AK3 | RBS | +/- 32 | 57 | 39 | August 27 |
B0034 | pSB1AK3 | RBS | +/- 50 | 29.6 | 40 | August 27 |
C0012 | pSB1A2 | LacI | +/- 30 | 133.1 | 21 | July 29 |
C0012 | pSB1A2 | LacI repressor | +/- 40 | 146.4 | 64 | July 29 |
C0012 | pSB1A2 | LacI repressor | +/- 40 | 203.1 | 50 | July 29 |
C0040 | pSB1A2 | TetR repressor | +/- 20 | 177.1 | 8 | July 24 |
C0040 | pSB1A2 | TetR repressor | +/- 30 | 161.7 | 9 | July 24 |
C0060 | pSB1A2 | lactonase production | +/- 48 | 84.2 | 54 | August 11 |
C0062 | pSB1A2 | LuxR repressor | +/- 48 | 76.2 | 55 | August 11 |
C0062 | pSB1AK3 | LuxR repressor | +/- 25 | 41.5 | 45 | August 27 |
C0062 | pSB1AK3 | LuxR repressor | +/- 50 | 31.7 | 49 | August 27 |
E0022 | pSB1A2 | eCFP | +/- 40 | 84.2 | 19 | July 23 |
E0022 | pSB1A2 | eCFP | +/- 40 | 86.4 | 20 | July 24 |
F1610 | pSB1AK3 | HSL Sender Device | +/- 50 | 57.6 | 17 | August 12 |
F1610 | pSB1AK3 | HSL Sender Device | +/- 40 | 53.1 | 18 | August 12 |
F1610 | pSB1AK3 | HSL Sender Device | +/- 50 | 41.4 | 22 | August 27 |
F1610 | pSB1AK3 | HSL Sender Device | +/- 20 | 31.5 | 33 | August 27 |
I12006 | pSB2K3 | 434 cI repressible promoter | +/- 48 | 25.8 | 51 | August 08 |
I12006 | pSB2K3 | 434 cI repressible promoter | +/- 48 | 52.7 | 52 | August 08 |
I712022 | BBa_J52017 (Km, Ap) | T7 polymerase | +/- 40 | 394.1 | 35 | July 31 |
I712074 | BBa_J52017 (Ap,Km) | T7 Promotor | +/- 42.5 | 208.2 | 27 | July 29 |
I712074 | BBa_J52017 (Ap,Km) | T7 Promotor | +/- 45 | 20.5 | 61 | August 27 |
I712074 | BBa_J52017 (Ap,Km) | T7 Promotor | +/- 45 | 63.9 | 62 | August 27 |
J23022 | BBa_J23006 (Ap) | 3d key+ terminator | +/- 40 | 160.7 | 25 | July 29 |
J23022 | BBa_J23006 (Ap) | 3d key+ terminator | +/- 46 | 308.3 | 29 | July 29 |
J23032 | BBa_J23006 (Ap) | 3d lock | +/- 35 | 117.4 | 23 | July 29 |
J23032 | BBa_J23006 (Ap) | 3d lock | +/- 39.5 | 144.8 | 24 | July 29 |
J23032 | BBa_J23006 (Ap) | 3d lock | +/- 48 | 129.1 | 28 | July 29 |
J23100 | BBa_J61002 (Ap) | Constitutive promoter | +/- 60 | 345.0 | 3 | July 24 |
J23100 | BBa_J61002 (Ap) | Constitutive promoter | +/- 30 | 176.1 | 4 | July 24 |
J23109 | BBa_J61002 (Ap) | Constitutive Promoter | +/- 46 | 375.1 | 26 | July 29 |
J23109 | BBa_J61002 (Ap) | Constitutive Promoter | +/- 48 | 99.2 | 59 | August 27 |
J23109 | BBa_J61002 (Ap) | Constitutive Promoter | +/- 48 | 69.2 | 60 | August 27 |
J23109 | BBa_J61002 (Ap) | Constitutive Promoter | +/- 48 | 404 | 30 | July 29 |
J23116 | BBa_J61002 (Ap) | Constitutive promoter | +/- 48 | 328.8 | 53 | August 08 |
M30109 | pSB1AC3 | Light-sensor | +/- 40 | 50.4 | 1 | July 23 |
M30109 | pSB1AC3 | Light-sensor | +/- 30 | 54.3 | 2 | July 24 |
R0011 | pSB1A2 | LacI regulated promoter | +/- 45 | 64.0 | 43 | July 29 |
R0011 | pSB1A2 | LacI regulated promoter | +/- 40 | 69.8 | 44 | July 29 |
R0011 | pSB1AK3 | LacI regulated promoter | +/- 50 | 30.8 | 38 | August 27 |
R0040 | pSB1A2 | tetR repressible promoter | +/- 48 | 37.9 | 63 | August 11 |
R0062 | pSB1A2 | LuxR regulated promoter | +/- 40 | 104.1 | 37 | July 31 |
R0084 | pSB1A2 | OmpF promoter | +/- 40 | 32.0 | 15 | July 23 |
R0084 | pSB1A2 | OmpF promoter | +/- 30 | 60.9 | 16 | July 24 |
MiniPrep Products: Ligations |
---|
Part | Plasmid | Description | µl left | ng/µl | Box | Date |
---|---|---|---|---|---|---|
C0062+B0015 | pSB1AK3 | Ligation for Cell Death | +/- 46 | 124.8 | 1A | August 08 |
E0022+B0015 | pSB1AK3 | Ligation for Output | +/- 46 | 151.8 | 5A | August 08 |
I712074+J23032 | BBa_J23006 (Ap) | Ligation for Filter | +/- 46 | 89.9 | 3A | August 08 |
J23109+J23032 | BBa_J23006 (Ap) | Ligation for Filter | +/- 46 | 94.4 | 4A | August 08 |
J23116+B0032 | pSB1A2 | Ligation for Input | +/- 40 | 102.5 | 8A | August 25 |
J23116+B0032 | pSB1A2 | Ligation for Input | +/- 35 | 96.2 | 2B | August 25 |
K145015+pSB1A2 | pSB1A2 | GFP with LVA tag | +/- 46 | 152.7 | 2A | August 08 |
K145151+pSB1A2 | pSB1A2 | ccdB coding region | +/- 46 | 57.8 | 5B | August 28 |
K145151+pSB1A2 | pSB1A2 | ccdB coding region | +/- 46 | 72.1 | 8B | August 28 |
K145151+pSB1A2 | pSB1A2 | ccdB coding region | +/- 46 | 18.5 | 7B | August 28 |
K145151+pSB1A2 | pSB1A2 | ccdB coding region | +/- 46 | 23.3 | 6B | August 28 |
R0040+B0032 | pSB1A2 | Ligation for output | +/- 30 | 64.4 | 9A | August 25 |
R0040+B0032 | pSB1A2 | Ligation for Output | +/- 35 | 93.1 | 1B | August 25 |
R0040+J23022 | BBa_J23006 (Ap) | Ligation for Filter (old) | +/- 35 | 81.3 | 3B | August 27 |
R0040+J23022 | BBa_J23006 (Ap) | Ligation for Filter (old) | +/- 35 | 86.3 | 4B | August 27 |
R0084+J23022+J23109+J23032 | BBa_J23006 (Ap) | Ligation for Filter (old) | +/- 20 | 150.2 | 6A | August 20 |
R0084+J23022+J23109+J23032 | BBa_J23006 (Ap) | Ligation for Filter (old) | +/- 20 | 58.3 | 7A | August 20 |
Restriction Digests |
---|
Part | Enzyme 1 | Enzyme 2 | vector/insert | length (bp) | µl left | ng/µl | Box | Date |
---|---|---|---|---|---|---|---|---|
B0015 | Pst I | Xba I | Insert | 144 | 10 | 4.6 | 40 | August 29 |
B0015 | EcoR I | Xba I | Vector | 3303 | 11 | 11.7 | 11 | July 31 |
B0015 | EcoR I | Xba I | Vector | 3303 | 10 | 13.6 | 17 | August 26 |
B0032 | EcoR I | Xba I | Vector | 2092 | 5 | 17.6 | 25 | August 19 |
B0032 | EcoR I | Xba I | Vector | 2092 | 20 | 6.4 | 46 | August 29 |
B0033 | EcoR I | Xba I | Vector | 2092 | 20 | 10.9 | 39 | August 27 |
C0040 | EcoR I | Spe I | Insert | 677 | 18 | 7.1 | 2 | July 24 |
C0012 | Spe I | Pst I | Vector | 3192 | 25 | 16.4 | 47 | August 29 |
C0060 | Spe I | Pst I | Vector | 2853 | 25 | 16.3 | 48 | August 29 |
C0040 | Spe I | Pst I | Vector | 2724 | 25 | 19.2 | 49 | August 29 |
K145015 | Spe I | Pst I | Vector | 2823 | 25 | 18.8 | 50 | August 29 |
K145151 | Spe I | Pst I | Vector | 2370 | 25 | 14.5 | 51 | August 29 |
K145001 | Spe I | Pst I | Vector | 4782 | 20 | 6.2 | 52 | August 29 |
C0060 | EcoR I | Spe I | Insert | 803 | 28 | 5.4 | 20 | August 12 |
C0060 | EcoR I | Spe I | Insert | 806 | 9 | 8.2 | 15 | August 01 |
C0062 | EcoR I | Spe I | Insert | 774 | 18 | 24.7 | 14 | August 01 |
C0062 | EcoR I | Spe I | Insert | 774 | 25 | 8.3 | 44 | August 27 |
C0062 | EcoR I | Spe I | Insert | 774 | 28 | 5.0 | 10 | August 12 |
E0022 | EcoR I | Spe I | Insert | 779 | 22 | 9.5 | 4 | July 24 |
F1610 | EcoR I | Xba I | Vector | 3956 | 26 | 4.5 | 35 | August 19 |
J23032 | EcoR I | Xba I | Vector | 3090 | 2 | 12.9 | 8 | July 30 |
J23032 | EcoR I | Xba I | Vector | 3090 | 45 | 1.3 | 9 | July 31 |
J23032 | EcoR I | Xba I | Vector | 3308 | 25 | 5.1 | 22 | August 07 |
J23100 | EcoR I | Spe I | Insert | 52 | 38.1 | 2.6 | 1 | July 24 |
J23109 | EcoR I | Spe I | Insert | 52 | 21 | 4.2 | 5 | July 29 |
J23116 | EcoR I | Spe I | Insert | 52 | +/-20 | 8.1 | 26 | August 12 |
K145001 (T7 poly) | EcoR I | Spe I | Insert | 2732 | +/-8 | 14.1 | 27 | August 12 |
K145001 | EcoR I | Spe I | Insert | 2730 | +/-21 | 24.3 | 24 | August 22 |
K145151 | EcoR I | Spe I | Insert | 306 | 19 | 25.2 | 36 | August 19 |
pSB1A2 | EcoR I | Spe I | Plasmid | 2079 | 9 | 18.2 | 16 | August 01 |
pSB1A2 | EcoR I | Spe I | Plasmid | 2056 | 24 | 27.7 | 43 | August 25 |
R0011 | EcoR I | Spe I | Insert | 72 | 20 | 8.5 | 12 | July 30 |
R0011 | EcoR I | Spe I | Insert | 72 | 20 | 7.5 | 13 | July 30 |
R0011 | EcoR I | Spe I | Insert | 72 | 25 | 2.1 | 21 | August 27 |
R0062 | EcoR I | Spe I | Insert | 55 | 27 | 7.8 | 23 | August 07 |
R0084 | EcoR I | Spe I | Insert | 125 | 33.4 | 3.8 | 3 | July 24 |
R0084 | EcoR I | Spe I | Insert | 125 | 25 | 6.1 | 6 | July 29 |
I712074 | EcoR I | Spe I | Insert | 61 | 25 | 3.4 | 7 | July 29 |
C0062+B0015 | EcoR I | Xba I | Vector | 4080 | +/-30 | 32.8 | 33 | August 14 |
E0022+B0015 | EcoR I | Xba I | Vector | 4086 | +/-30 | 14.8 | 32 | August 14 |
I712074+J23032 | EcoR I | Spe I | Insert | 112 | +/-30 | 3.9 | 28 | August 14 |
J23109+J23032 | EcoR I | Spe I | Insert | 101 | +/-30 | 4.0 | 29 | August 14 |
J23109+J23032 | EcoR I | Xba I | Vector | 3113 | +/-20 | 8.3 | 31 | August 14 |
J23116+B0032 | EcoR I | Spe I | Insert | 71 | +/-28 | 2.5 | 41 | August 25 |
R0084+B0032 | EcoR I | Spe I | Insert | 144 | +/-20 | 0.9 | 18 | August 22 |
R0084+B0032 | EcoR I | Spe I | Insert | 144 | +/-30 | 2.9 | 19 | August 21 |
R0040+B0032 | EcoR I | Spe I | Insert | 90 | +/-28 | 2.6 | 38 | August 25 |
R0040+J23022 | EcoR I | Spe I | Insert | 311 | +/-28 | 3.6 | 45 | August 27 |
R0084+B0032 | EcoR I | Spe I | Insert | 144 | +/-28 | 2.4 | 42 | August 25 |
R0084+J23022 | EcoR I | Spe I | Insert | 365 | +/-20 | 6.9 | 30 | August 14 |
R0084+J23022+J23109+J23032 | EcoR I | Spe I | Insert | 432 | +/-30 | 4.6 | 34 | August 22 |
R0084+J23022+J23109+J23032 | EcoR I | Spe I | Insert | 432 | +/-30 | 3.7 | 37 | August 22 |
Things stored in freezer in lab (-20°C)
Miscellaneous |
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Description | Name | µl left | Concentration | Box | Date |
---|---|---|---|---|---|
Primer GFP-LVA | 1084 | 90 | 20 µM | 1 | August 01 |
Primer GFP-LVA | 1085 | 90 | 20 µM | 2 | August 01 |
dNTP mix | ATP, GTP, TTP, CTP | 70 | 10 mM | 3 | August 01 |
Primer GFP-LVA | 1084 | 500 | 100 µM | 4 | August 01 |
Primer GFP-LVA | 1085 | 500 | 100 µM | 5 | August 01 |
Primer Test | 1074 | 800 | 100 µM | 6 | August 07 |
Primer Test | 1075 | 780 | 100 µM | 7 | August 07 |
Primer T7-UmuD | 1076 | 560 | 100 µM | 8 | August 07 |
Primer T7-UmuD | 1077 | 477 | 100 µM | 9 | August 07 |
Primer T7-UmuD | 1078 | 467 | 100 µM | 10 | August 07 |
Primer T7-UmuD | 1079 | 45 | 100 µM | 11 | August 07 |
Primer Transduction | 1097 | 627 | 100 µM | 12 | August 07 |
Primer Transduction | 1098 | 710 | 100 µM | 13 | August 07 |
Primer Transduction | 1099 | 608 | 100 µM | 14 | August 07 |
Primer Anti-sense LuxI | 1100 | 522 | 100 µM | 15 | August 07 |
Primer Anti-sense LuxI | 1101 | 237 | 100 µM | 16 | August 07 |
Primer Test | 1074 | 100 | 20 µM | 17 | August 07 |
Primer Test | 1075 | 100 | 20 µM | 18 | August 07 |
Primer T7-UmuD | 1076 | 100 | 20 µM | 19 | August 07 |
Primer T7-UmuD | 1077 | 100 | 20 µM | 20 | August 07 |
Primer T7-UmuD | 1078 | 100 | 20 µM | 21 | August 07 |
Primer T7-UmuD | 1079 | 100 | 20 µM | 22 | August 07 |
Primer Transduction | 1097 | 100 | 20 µM | 23 | August 07 |
Primer Transduction | 1098 | 100 | 20 µM | 24 | August 07 |
Primer Transduction | 1099 | 100 | 20 µM | 25 | August 07 |
Primer Anti-sense LuxI | 1100 | 100 | 20 µM | 26 | August 07 |
Primer Anti-sense LuxI | 1101 | 100 | 20 µM | 27 | August 07 |
dNTP mix | ATP, GTP, TTP, CTP | 90 | 2 mM | 28 | August 19 |
Primer ccdB | 1127 | 100 | 100 µM | 29 | August 19 |
Primer ccdB | 1128 | 100 | 100 µM | 30 | August 19 |
Primer K145150 | 1129 | 100 | 100 µM | 31 | August 19 |
Primer K145150 | 1130 | 100 | 100 µM | 32 | August 19 |
Primer ccdB | 1127 | 90 | 20 µM | 33 | August 19 |
Primer ccdB | 1128 | 980 | 20 µM | 34 | August 19 |
Primer K145150 | 1129 | 990 | 1 µM | 35 | August 19 |
Primer K145150 | 1130 | 990 | 1 µM | 36 | August 19 |
dNTP mix | ATP, GTP, TTP, CTP | 990 | 2 µM | 37 | August 19 |