Team:KULeuven/Freezer

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Revision as of 12:23, 31 August 2008

dock/undock dropdown

Parts stored in the fridge in the lab (4°C)

MiniPrep Products: Parts

Part	Plasmid	Description	µl left	ng/µl	Box	Date
B0015	pSB1AK3	Terminator	+/- 10	112.3	11	July 24
B0015	pSB1AK3	Terminator	+/- 20	130.3	12	July 24
B0015	pSB1AK3	Terminator	+/- 40	303.1	13	July 29
B0015	pSB1AK3	Terminator	+/- 20	213.6	14	July 29
B0015	pSB1AK3	Terminator	+/- 48	311.0	31	July 29
B0015	pSB1AK3	Terminator	+/- 30	214.0	47	July 29
B0015	pSB1AK3	Terminator	+/- 40	209.5	48	July 29
B0015	pSB1AK3	Terminator	+/- 40	63.0	10	July 23
B0032	pSB1A2	RBS(medium)	+/- 5	51.0	56	August 11
B0032	pSB1A2	RBS(medium)	+/- 20	47.2	57	August 27
B0032	pSB1A2	RBS(medium)	+/- 40	27.0	58	August 27
B0033	pSB1AK3	RBS	+/- 50	36.5	35	August 27
B0033	pSB1AK3	RBS	+/- 23	38.4	41	August 27
B0034	pSB1A2	RBS (efficiency 1)	+/- 20	73.8	5	July 24
B0034	pSB1A2	RBS (efficiency 1)	+/- 30	95.1	6	July 24
B0034	pSB1A2	RBS (efficiency 1)	+/- 40	104.0	7	July 29
B0034	pSB1AK3	RBS	+/- 15	57	39	August 27
B0034	pSB1AK3	RBS	+/- 50	29.6	40	August 27
C0012	pSB1A2	LacI	+/- 30	133.1	21	July 29
C0012	pSB1A2	LacI repressor	+/- 40	146.4	64	July 29
C0012	pSB1A2	LacI repressor	+/- 40	203.1	50	July 29
C0040	pSB1A2	TetR repressor	+/- 20	177.1	8	July 24
C0040	pSB1A2	TetR repressor	+/- 30	161.7	9	July 24
C0060	pSB1A2	lactonase production	+/- 48	84.2	54	August 11
C0062	pSB1A2	LuxR repressor	+/- 48	76.2	55	August 11
C0062	pSB1AK3	LuxR repressor	+/- 25	41.5	45	August 27
C0062	pSB1AK3	LuxR repressor	+/- 50	31.7	49	August 27
E0022	pSB1A2	eCFP	+/- 40	84.2	19	July 23
E0022	pSB1A2	eCFP	+/- 40	86.4	20	July 24
F1610	pSB1AK3	HSL Sender Device	+/- 50	57.6	17	August 12
F1610	pSB1AK3	HSL Sender Device	+/- 40	53.1	18	August 12
F1610	pSB1AK3	HSL Sender Device	+/- 50	41.4	22	August 27
F1610	pSB1AK3	HSL Sender Device	+/- 20	31.5	33	August 27
I12006	pSB2K3	434 cI repressible promoter	+/- 48	25.8	51	August 08
I12006	pSB2K3	434 cI repressible promoter	+/- 48	52.7	52	August 08
I712022	BBa_J52017 (Km, Ap)	T7 polymerase	+/- 40	394.1	35	July 31
I712074	BBa_J52017 (Ap,Km)	T7 Promotor	+/- 42.5	208.2	27	July 29
I712074	BBa_J52017 (Ap,Km)	T7 Promotor	+/- 45	20.5	61	August 27
I712074	BBa_J52017 (Ap,Km)	T7 Promotor	+/- 45	63.9	62	August 27
J23022	BBa_J23006 (Ap)	3d key+ terminator	+/- 40	160.7	25	July 29
J23022	BBa_J23006 (Ap)	3d key+ terminator	+/- 46	308.3	29	July 29
J23032	BBa_J23006 (Ap)	3d lock	+/- 35	117.4	23	July 29
J23032	BBa_J23006 (Ap)	3d lock	+/- 39.5	144.8	24	July 29
J23032	BBa_J23006 (Ap)	3d lock	+/- 48	129.1	28	July 29
J23100	BBa_J61002 (Ap)	Constitutive promoter	+/- 60	345.0	3	July 24
J23100	BBa_J61002 (Ap)	Constitutive promoter	+/- 30	176.1	4	July 24
J23109	BBa_J61002 (Ap)	Constitutive Promoter	+/- 46	375.1	26	July 29
J23109	BBa_J61002 (Ap)	Constitutive Promoter	+/- 48	99.2	59	August 27
J23109	BBa_J61002 (Ap)	Constitutive Promoter	+/- 48	69.2	60	August 27
J23109	BBa_J61002 (Ap)	Constitutive Promoter	+/- 48	404	30	July 29
J23116	BBa_J61002 (Ap)	Constitutive promoter	+/- 48	328.8	53	August 08
M30109	pSB1AC3	Light-sensor	+/- 40	50.4	1	July 23
M30109	pSB1AC3	Light-sensor	+/- 30	54.3	2	July 24
R0011	pSB1A2	LacI regulated promoter	+/- 45	64.0	43	July 29
R0011	pSB1A2	LacI regulated promoter	+/- 40	69.8	44	July 29
R0011	pSB1AK3	LacI regulated promoter	+/- 50	30.8	38	August 27
R0040	pSB1A2	tetR repressible promoter	+/- 48	37.9	63	August 11
R0062	pSB1A2	LuxR regulated promoter	+/- 40	104.1	37	July 31
R0084	pSB1A2	OmpF promoter	+/- 40	32.0	15	July 23
R0084	pSB1A2	OmpF promoter	+/- 30	60.9	16	July 24
C0060	pSB1A2	Lactonase	30	155.3	65	July 31
C0062	pSB1A2	LuxR repressor/activator	?	159.4	32	July 31
R0011	pSB1A2	lacI + pL promoter	?	68.0	34	July 31
J23066	J23006	key	+/- 45	541	66	August 30
P0353	pSB1AK3	cII p22 van memory	+/- 45	406.9	67	August 30
P1052	pSB1A2	cI 434 van memory	+/- 44	265.4	68	August 30
E0240	pSB1A2	GFP generator	+/- 44	281	69	August 30
K145015	pSB1A2	GFP+LVA	+/- 48	257	70	August 30
K145151	pSB1A2	ccdB	+/- 48	204.6	71	August 30
K145001	pSB1A2	T7 without UmuD	+/- 48	414.8	72	August 30
C0056	pSB1A2	cI represor	+/- 45	419.8	73	August 30
C0061 C0061	pSB1A2	autoinducer ASL synthetase	+/- 45	338.6	74	August 30
B0014	pSB1AK3	terminator	+/- 45	344.4	75	August 30
E0040	pSB1A2	GFP	+/- 48	305.8	76	August 30	- style="background: #E0FFFF; text-align: center;"	K145151	pSB1A2	ccdB	+/- 48	188.4	77	August 30

MiniPrep Products: Ligations

Part	Plasmid	Description	µl left	ng/µl	Box	Date
C0062+B0015	pSB1AK3	Ligation for Cell Death	+/- 46	124.8	1A	August 08
E0022+B0015	pSB1AK3	Ligation for Output	+/- 46	151.8	5A	August 08
I712074+J23032	BBa_J23006 (Ap)	Ligation for Filter	+/- 46	89.9	3A	August 08
J23109+J23032	BBa_J23006 (Ap)	Ligation for Filter	+/- 46	94.4	4A	August 08
J23116+B0032	pSB1A2	Ligation for Input	+/- 40	102.5	8A	August 25
J23116+B0032	pSB1A2	Ligation for Input	+/- 35	96.2	2B	August 25
K145015+pSB1A2	pSB1A2	GFP with LVA tag	+/- 46	152.7	2A	August 08
K145151+pSB1A2	pSB1A2	ccdB coding region	+/- 46	57.8	5B	August 28
K145151+pSB1A2	pSB1A2	ccdB coding region	+/- 46	72.1	8B	August 28
K145151+pSB1A2	pSB1A2	ccdB coding region	+/- 46	18.5	7B	August 28
K145151+pSB1A2	pSB1A2	ccdB coding region	+/- 46	23.3	6B	August 28
R0040+B0032	pSB1A2	Ligation for output	+/- 30	64.4	9A	August 25
R0040+B0032	pSB1A2	Ligation for Output	+/- 35	93.1	1B	August 25
R0040+J23022	BBa_J23006 (Ap)	Ligation for Filter (old)	+/- 35	81.3	3B	August 27
R0040+J23022	BBa_J23006 (Ap)	Ligation for Filter (old)	+/- 35	86.3	4B	August 27
R0084+J23022+J23109+J23032	BBa_J23006 (Ap)	Ligation for Filter (old)	+/- 20	150.2	6A	August 20
R0084+J23022+J23109+J23032	BBa_J23006 (Ap)	Ligation for Filter (old)	+/- 20	58.3	7A	August 20

Restriction Digests

Part	Enzyme 1	Enzyme 2	vector/insert	length (bp)	µl left	ng/µl	Box	Date
B0015	Pst I	Xba I	Insert	144	10	4.6	40	August 29
B0015	EcoR I	Xba I	Vector	3303	11	11.7	11	July 31
B0015	EcoR I	Xba I	Vector	3303	10	13.6	17	August 26
B0032	EcoR I	Xba I	Vector	2092	5	17.6	25	August 19
B0032	EcoR I	Xba I	Vector	2092	20	6.4	46	August 29
B0033	EcoR I	Xba I	Vector	2092	20	10.9	39	August 27
C0040	EcoR I	Spe I	Insert	677	18	7.1	2	July 24
C0012	Spe I	Pst I	Vector	3192	25	16.4	47	August 29
C0060	Spe I	Pst I	Vector	2853	25	16.3	48	August 29
C0040	Spe I	Pst I	Vector	2724	25	19.2	49	August 29
K145015	Spe I	Pst I	Vector	2823	25	18.8	50	August 29
K145151	Spe I	Pst I	Vector	2370	25	14.5	51	August 29
K145001	Spe I	Pst I	Vector	4782	20	6.2	52	August 29
C0060	EcoR I	Spe I	Insert	803	28	5.4	20	August 12
C0060	EcoR I	Spe I	Insert	806	9	8.2	15	August 01
C0062	EcoR I	Spe I	Insert	774	18	24.7	14	August 01
C0062	EcoR I	Spe I	Insert	774	25	8.3	44	August 27
C0062	EcoR I	Spe I	Insert	774	28	5.0	10	August 12
E0022	EcoR I	Spe I	Insert	779	22	9.5	4	July 24
F1610	EcoR I	Xba I	Vector	3956	26	4.5	35	August 19
J23032	EcoR I	Xba I	Vector	3090	2	12.9	8	July 30
J23032	EcoR I	Xba I	Vector	3090	45	1.3	9	July 31
J23032	EcoR I	Xba I	Vector	3308	25	5.1	22	August 07
J23100	EcoR I	Spe I	Insert	52	38.1	2.6	1	July 24
J23109	EcoR I	Spe I	Insert	52	21	4.2	5	July 29
J23116	EcoR I	Spe I	Insert	52	+/-20	8.1	26	August 12
K145001 (T7 poly)	EcoR I	Spe I	Insert	2732	+/-8	14.1	27	August 12
K145001	EcoR I	Spe I	Insert	2730	+/-21	24.3	24	August 22
K145151	EcoR I	Spe I	Insert	306	19	25.2	36	August 19
pSB1A2	EcoR I	Spe I	Plasmid	2079	9	18.2	16	August 01
pSB1A2	EcoR I	Spe I	Plasmid	2056	24	27.7	43	August 25
R0011	EcoR I	Spe I	Insert	72	20	8.5	12	July 30
R0011	EcoR I	Spe I	Insert	72	20	7.5	13	July 30
R0011	EcoR I	Spe I	Insert	72	25	2.1	21	August 27
R0062	EcoR I	Spe I	Insert	55	27	7.8	23	August 07
R0084	EcoR I	Spe I	Insert	125	33.4	3.8	3	July 24
R0084	EcoR I	Spe I	Insert	125	25	6.1	6	July 29
I712074	EcoR I	Spe I	Insert	61	25	3.4	7	July 29
C0062+B0015	EcoR I	Xba I	Vector	4080	+/-30	32.8	33	August 14
E0022+B0015	EcoR I	Xba I	Vector	4086	+/-30	14.8	32	August 14
I712074+J23032	EcoR I	Spe I	Insert	112	+/-30	3.9	28	August 14
J23109+J23032	EcoR I	Spe I	Insert	101	+/-30	4.0	29	August 14
J23109+J23032	EcoR I	Xba I	Vector	3113	+/-20	8.3	31	August 14
J23116+B0032	EcoR I	Spe I	Insert	71	+/-28	2.5	41	August 25
R0084+B0032	EcoR I	Spe I	Insert	144	+/-20	0.9	18	August 22
R0084+B0032	EcoR I	Spe I	Insert	144	+/-30	2.9	19	August 21
R0040+B0032	EcoR I	Spe I	Insert	90	+/-28	2.6	38	August 25
R0040+J23022	EcoR I	Spe I	Insert	311	+/-28	3.6	45	August 27
R0084+B0032	EcoR I	Spe I	Insert	144	+/-28	2.4	42	August 25
R0084+J23022	EcoR I	Spe I	Insert	365	+/-20	6.9	30	August 14
R0084+J23022+J23109+J23032	EcoR I	Spe I	Insert	432	+/-30	4.6	34	August 22
R0084+J23022+J23109+J23032	EcoR I	Spe I	Insert	432	+/-30	3.7	37	August 22

Things stored in freezer in lab (-20°C)

Miscellaneous

Description	Name	µl left	Concentration	Box	Date
Primer GFP-LVA	1084	90	20 µM	1	August 01
Primer GFP-LVA	1085	90	20 µM	2	August 01
dNTP mix	ATP, GTP, TTP, CTP	70	10 mM	3	August 01
Primer GFP-LVA	1084	500	100 µM	4	August 01
Primer GFP-LVA	1085	500	100 µM	5	August 01
Primer Test	1074	800	100 µM	6	August 07
Primer Test	1075	780	100 µM	7	August 07
Primer T7-UmuD	1076	560	100 µM	8	August 07
Primer T7-UmuD	1077	477	100 µM	9	August 07
Primer T7-UmuD	1078	467	100 µM	10	August 07
Primer T7-UmuD	1079	45	100 µM	11	August 07
Primer Transduction	1097	627	100 µM	12	August 07
Primer Transduction	1098	710	100 µM	13	August 07
Primer Transduction	1099	608	100 µM	14	August 07
Primer Anti-sense LuxI	1100	522	100 µM	15	August 07
Primer Anti-sense LuxI	1101	237	100 µM	16	August 07
Primer Test	1074	100	20 µM	17	August 07
Primer Test	1075	100	20 µM	18	August 07
Primer T7-UmuD	1076	100	20 µM	19	August 07
Primer T7-UmuD	1077	100	20 µM	20	August 07
Primer T7-UmuD	1078	100	20 µM	21	August 07
Primer T7-UmuD	1079	100	20 µM	22	August 07
Primer Transduction	1097	100	20 µM	23	August 07
Primer Transduction	1098	100	20 µM	24	August 07
Primer Transduction	1099	100	20 µM	25	August 07
Primer Anti-sense LuxI	1100	100	20 µM	26	August 07
Primer Anti-sense LuxI	1101	100	20 µM	27	August 07
dNTP mix	ATP, GTP, TTP, CTP	90	2 mM	28	August 19
Primer ccdB	1127	100	100 µM	29	August 19
Primer ccdB	1128	100	100 µM	30	August 19
Primer K145150	1129	100	100 µM	31	August 19
Primer K145150	1130	100	100 µM	32	August 19
Primer ccdB	1127	90	20 µM	33	August 19
Primer ccdB	1128	980	20 µM	34	August 19
Primer K145150	1129	990	1 µM	35	August 19
Primer K145150	1130	990	1 µM	36	August 19
dNTP mix	ATP, GTP, TTP, CTP	990	2 µM	37	August 19

@@ Line 151: / Line 151: @@
 |- style="background: #E0FFFF; text-align: center;"
 |[http://partsregistry.org/Part:BBa_E0240 E0240]||pSB1A2||GFP generator||+/- 44||281||69||August 30
+|- style="background: #E0FFFF; text-align: center;"
+|[http://partsregistry.org/Part:BBa_K145015 K145015]||pSB1A2||GFP+LVA||+/- 48||257||70||August 30
+|- style="background: #E0FFFF; text-align: center;"
+|[http://partsregistry.org/Part:BBa_K145151 K145151]||pSB1A2||ccdB||+/- 48||204.6||71||August 30
+|- style="background: #E0FFFF; text-align: center;"
+|[http://partsregistry.org/Part:BBa_K145001 K145001]||pSB1A2||T7 without UmuD||+/- 48||414.8||72||August 30
+|- style="background: #E0FFFF; text-align: center;"
+|[http://partsregistry.org/Part:BBa_C0056 C0056]||pSB1A2||cI represor||+/- 45||419.8||73||August 30
+|- style="background: #E0FFFF; text-align: center;"
+|[http://partsregistry.org/Part:BBa_ C0061 C0061]||pSB1A2||autoinducer ASL synthetase||+/- 45||338.6||74||August 30
+|- style="background: #E0FFFF; text-align: center;"
+|[http://partsregistry.org/Part:BBa_B0014 B0014]||pSB1AK3||terminator||+/- 45||344.4||75||August 30
+|- style="background: #E0FFFF; text-align: center;"
+|[http://partsregistry.org/Part:BBa_E0040 E0040]||pSB1A2||GFP||+/- 48||305.8||76||August 30
+||- style="background: #E0FFFF; text-align: center;"
+|[http://partsregistry.org/Part:BBa_K145151 K145151]||pSB1A2||ccdB||+/- 48||188.4||77||August 30
 |}