Team:KULeuven/Ligation

From 2008.igem.org

(Difference between revisions)
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(→Ligations: step 1)
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{{:Team:KULeuven/Tools/Header}}
{{:Team:KULeuven/Tools/Header}}
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== Ligations: step 1 ==
+
== New Parts ==
-
{|
+
 
-
|- style="background: #003E81;color:white;width=100%"
+
{| style="background:#99CCFF; color:black; width:100%; text-align:center;" border="1" cellpadding="2" cellspacing="0"
-
! colspan="6" style="background: #003E81" | Input
+
! BioBrick !! Description !! Status !! Experience
-
|- style="background: #99CCFF; text-align: center; width=100%"
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|width=200| '''Name'''
+
| [http://partsregistry.org/Part:BBa_K145001 K145001] || T7 DNA polymerase (no tag)|| [[Image:correct.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|width=160| '''Miniprep'''
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|width=150| '''Digest'''
+
| [http://partsregistry.org/Part:BBa_K145013 K145013] || antisense LuxI|| electroporation || [[Image:wrong.JPG|center|30px]]
-
|width=150| '''Ligation'''
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|width=150| '''Colonies'''
+
| [http://partsregistry.org/Part:BBa_K145014 K145014] || T7 DNA polymerase with UmuD tag|| [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|width=150| '''Confirmed'''
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|- style="background:#ffffff; color:black;  text-align:center;"
-
|- style="background: #E0FFFF; text-align: center;"  
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| [http://partsregistry.org/Part:BBa_K145015 K145015] || GFP with LVA tag|| [[Image:correct.JPG|center|30px]] || [[Image:correct.JPG|center|30px]]
-
|J23116+B0032||ok||ok||-||-||-
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145150 K145150] || hybrid promoter|| electroporation || [[Image:wrong.JPG|center|30px]]
-
|C0040+B0015||ok||ok||-||ok||-
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|- style="background:#ffffff; color:black;  text-align:center;"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145151 K145151] || ccdB coding region || [[Image:correct.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|R0040+E0240||-||-||-||-||-
+
|}
-
|- style="background: #003E81;color:white;width=100%"
+
 
-
! colspan="6" style="background: #003E81" | Output
+
 
-
|- style="background: #99CCFF; text-align: center; width=100%"
+
==New Devices ==
-
|width=200| '''Name'''
+
 
-
|width=160| '''Miniprep'''
+
{| style="background:#99CCFF; color:black; width:100%; text-align:center;" border="1" cellpadding="2" cellspacing="0"
-
|width=150| '''Digest'''
+
! BioBrick !! Components !! Description !! Status !! Experience
-
|width=150| '''Ligation'''
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|width=150| '''Colonies'''
+
| [http://partsregistry.org/Part:BBa_K145201 K145201] || J23116+B0032+C0040+B0015 || Input || [[Image:correct.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|width=150| '''Confirmed'''
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145205 K145205] || R0040+B0032+K145015+B0015 || Output (LVA tag)|| [[Image:correct.JPG|center|30px]] || [[Image:correct.JPG|center|30px]]
-
| R0084+B0032||ok||ok||-||-||-
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145252 K145252] || R0040+E0240 || Output (no tag) || [[Image:correct.JPG|center|30px]] || [[Image:correct.JPG|center|30px]]
-
| E0022+B0015||ok||ok||ok||ok||ok
+
|- style="background:#ffffff; color:black; text-align:center;"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145280 K145280] || K145201+K145205 || Input-Output: test || ligation mix || [[Image:wrong.JPG|center|30px]]
-
| K145015+B0015||pcr||ok||ok||ok||-
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|- style="background:#ffffff; color:black;  text-align:center;"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145279 K145279] || K145201+K145252 || Input-Output: test || ligation mix|| [[Image:wrong.JPG|center|30px]]
-
| E0040+B0015||-||-||-||-||-
+
|- style="background:#ffffff; color:black; text-align:center;"
-
|- style="background: #003E81;color:white;width=100%"
+
| [http://partsregistry.org/Part:BBa_K145258 K145258] || R0040+B0033+C0056+B0015 || Memory - part 1 || ligation mix|| [[Image:wrong.JPG|center|30px]]
-
! colspan="6" style="background: #003E81" | Memory
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|- style="background:#ffffff; color:black;  text-align:center;"
-
|- style="background: #99CCFF; text-align: center; width=100%"
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| [http://partsregistry.org/Part:BBa_K145259 K145259] || R0053+P0152+R1052+P0353 || Memory - part 2 || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|width=200| '''Name'''
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|width=160| '''Miniprep'''
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| [http://partsregistry.org/Part:BBa_K145210 K145210] || K145258+K145259 || Memory || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|width=150| '''Digest'''
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|- style="background:#ffffff; color:black;  text-align:center;"
-
|width=150| '''Ligation'''
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| [http://partsregistry.org/Part:BBa_K145211 K145211] || R1052+K145013+B0015 || Memory - part 3 || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|width=150| '''Colonies'''
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|width=150| '''Confirmed'''
+
| [http://partsregistry.org/Part:BBa_K145215 K145215] || R0040+J23066 || Filter - part 1 || [[Image:correct.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|- style="background: #E0FFFF; text-align: center;"
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|- style="background:#ffffff; color:black;  text-align:center;"
-
| R0053+P0152 ||-||-||-||-||-
+
| [http://partsregistry.org/Part:BBa_K145278 K145278] || J23109+J23032+K145001+B0015 || Filter (old) - part 2 || ligation mix|| [[Image:wrong.JPG|center|30px]]
-
|- style="background: #E0FFFF; text-align: center;"
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
| R1052+P0353 ||-||-||-||-||-
+
| [http://partsregistry.org/Part:BBa_K145277 K145277] || J23109+J23078+K145001+B0015 || Filter (new) - part 2 || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|- style="background: #E0FFFF; text-align: center;"
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|- style="background:#ffffff; color:black;  text-align:center;"
-
| R0084+B0033 ||-||-||-||-||-
+
| [http://partsregistry.org/Part:BBa_K145275 K145275] || K145215+K145278 || Filter (old) || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|- style="background: #E0FFFF; text-align: center;"
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
| C0056+B0015 ||-||-||-||-||-
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| [http://partsregistry.org/Part:BBa_K145276 K145276] || K145215+K145277 || Filter (new) || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|- style="background: #E0FFFF; text-align: center;"
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|- style="background:#ffffff; color:black;  text-align:center;"
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| R1052+K145013 ||-||-||-||-||-
+
| [http://partsregistry.org/Part:BBa_K145253 K145253] || I712074+J23032+C0012+B0015 || Invertimer (old) - part 1 || [[Image:correct.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|- style="background: #E0FFFF; text-align: center;"
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|- style="background:#ffffff; color:black;  text-align:center;"
-
| K145013+B0015 ||-||-||-||-||-
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| [http://partsregistry.org/Part:BBa_K145255 K145255] || I712074+J23078+C0012+B0015 || Invertimer (new) - part 1 || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|- style="background: #003E81;color:white;width=100%"
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|- style="background:#ffffff; color:black;  text-align:center;"
-
! colspan="6" style="background: #003E81" | Filter
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| [http://partsregistry.org/Part:BBa_K145254 K145254] || R0011+B0032+C0061+B0015 || Invertimer - part 2 || electroporation || [[Image:wrong.JPG|center|30px]]
-
|- style="background: #99CCFF; text-align: center; width=100%"
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|- style="background:#ffffff; color:black;  text-align:center;"
-
|width=200| '''Name'''
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| [http://partsregistry.org/Part:BBa_K145220 K145220] || K145255+K145254 || Invertimer (new) || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|width=160| '''Miniprep'''
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|width=150| '''Digest'''
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| [http://partsregistry.org/Part:BBa_K145104 K145104] || K145253+K145254 || Invertimer (old) || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|width=150| '''Ligation'''
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|- style="background:#ffffff; color:black; text-align:center;"
-
|width=150| '''Colonies'''
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| [http://partsregistry.org/Part:BBa_K145251 K145251] || I712074+J23032+C0060+B0015 || Reset (old) || [[Image:correct.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|width=150| '''Confirmed'''
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|- style="background: #E0FFFF; text-align: center;"
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| [http://partsregistry.org/Part:BBa_K145225 K145225] || I712074+J23078+C0060+B0015 || Reset (new) || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
| R0084+J23022 ||ok||ok||ok||ok||ok
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145256 K145256] || K145150+B0034+C0062 || Cell Death - part 1 || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
| J23109+J23032 ||ok||ok||ok||ok||ok
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145257 K145257] || B0014+B0033+K145151+B0015 || Cell Death - part 2 || electroporation || [[Image:wrong.JPG|center|30px]]
-
| K145014+B0015 ||-||-||-||-||-
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145230 K145230] || K145256+K145257 || Cell Death || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
| K145001+B0015 ||pcr||ok||-||-||-
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145273 K145273] || R0040+P0353 || test K145150 - part 2 || electroporation || [[Image:wrong.JPG|center|30px]]
-
| I7122074+J23032 ||ok||ok||ok||ok||ok
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145270 K145270] || J23116+B0032+C0062+B0015 || test K145150 - part 3 || ligation mix || [[Image:wrong.JPG|center|30px]]
-
| K145015+B0015 ||pcr||ok||ok||ok||-
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|- style="background: #E0FFFF; text-align: center;"
+
| [http://partsregistry.org/Part:BBa_K145269 K145269] || K145150+E0240 || test K145150 - part 4 || electroporation || [[Image:wrong.JPG|center|30px]]
-
| E0040+B0015 ||-||-||-||-||-
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|- style="background: #003E81;color:white;width=100%"
+
| [http://partsregistry.org/Part:BBa_K145268 K145268] || K145201+K145273 || test K145150 - part 5 || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
! colspan="6" style="background: #003E81" | Inverter
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|- style="background:#ffffff; color:black; text-align:center;"
-
|- style="background: #99CCFF; text-align: center; width=100%"
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| [http://partsregistry.org/Part:BBa_K145267 K145267] || K145270+K145269 || test K145150 - part 6 || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|width=200| '''Name'''
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|width=160| '''Miniprep'''
+
| [http://partsregistry.org/Part:BBa_K145266 K145266] || K145268+K145267 || test K145150|| [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|width=150| '''Digest'''
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|width=150| '''Ligation'''
+
| [http://partsregistry.org/Part:BBa_K145271 K145271] || I712074+J23078+E0040+B0015 || test filter (new) - part 3 || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|width=150| '''Colonies'''
+
|- style="background:#ffffff; color:black;  text-align:center;"
-
|width=150| '''Confirmed'''
+
| [http://partsregistry.org/Part:BBa_K145272 K145272] || I712074+J23032+E0040+B0015 || test filter (old) - part 3 || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|- style="background: #E0FFFF; text-align: center;"
+
|- style="background:#ffffff; color:black;  text-align:center;"
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| I712074+J23032||ok||ok||ok||ok||ok
+
| [http://partsregistry.org/Part:BBa_K145265 K145265] || K145275+K145272 || test filter (old) || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|- style="background: #E0FFFF; text-align: center;"
+
|- style="background:#ffffff; color:black;  text-align:center;"
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| C0012+B0015||ok||ok||ok||ok||-
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| [http://partsregistry.org/Part:BBa_K145264 K145264] || K145276+K145271 || test filter (new) || [[Image:wrong.JPG|center|30px]] || [[Image:wrong.JPG|center|30px]]
-
|- style="background: #E0FFFF; text-align: center;"
+
-
| R0011+F1610||ok||-||-||-||-
+
-
|- style="background: #E0FFFF; text-align: center;"
+
-
| R0011+E0240||-||-||-||-||-
+
-
|- style="background: #003E81;color:white;width=100%"
+
-
! colspan="6" style="background: #003E81" | Reset
+
-
|- style="background: #99CCFF; text-align: center; width=100%"
+
-
|width=200| '''Name'''
+
-
|width=160| '''Miniprep'''
+
-
|width=150| '''Digest'''
+
-
|width=150| '''Ligation'''
+
-
|width=150| '''Colonies'''
+
-
|width=150| '''Confirmed'''
+
-
|- style="background: #E0FFFF; text-align: center;"
+
-
| I712074+J023022||ok||ok||ok||ok||ok
+
-
|- style="background: #E0FFFF; text-align: center;"
+
-
| C0060+B0015||ok||ok||ok||ok||-
+
-
|- style="background: #003E81;color:white;width=100%"
+
-
! colspan="6" style="background: #003E81" | Cell Death
+
-
|- style="background: #99CCFF; text-align: center; width=100%"
+
-
|width=200| '''Name'''
+
-
|width=160| '''Miniprep'''
+
-
|width=150| '''Digest'''
+
-
|width=150| '''Ligation'''
+
-
|width=150| '''Colonies'''
+
-
|width=150| '''Confirmed'''
+
-
|- style="background: #E0FFFF; text-align: center;"
+
-
| J23100+B0032||ok||ok||ok||ok||-
+
-
|- style="background: #E0FFFF; text-align: center;"
+
-
| C0062+B0015||ok||ok||ok||ok||ok
+
-
|- style="background: #E0FFFF; text-align: center;"
+
-
| R0062+B0032||ok||ok||ok||ok||ok
+
-
|- style="background: #E0FFFF; text-align: center;"
+
-
| P1010+B0015||-||-||-||-||-
+
-
|- style="background: #E0FFFF; text-align: center;"
+
-
| R0062+E0240||-||-||-||-||-
+
|}
|}

Revision as of 00:23, 8 September 2008

  dock/undock dropdown  

New Parts

BioBrick Description Status Experience
K145001 T7 DNA polymerase (no tag)
Correct.JPG
Wrong.JPG
K145013 antisense LuxI electroporation
Wrong.JPG
K145014 T7 DNA polymerase with UmuD tag
Wrong.JPG
Wrong.JPG
K145015 GFP with LVA tag
Correct.JPG
Correct.JPG
K145150 hybrid promoter electroporation
Wrong.JPG
K145151 ccdB coding region
Correct.JPG
Wrong.JPG


New Devices

BioBrick Components Description Status Experience
K145201 J23116+B0032+C0040+B0015 Input
Correct.JPG
Wrong.JPG
K145205 R0040+B0032+K145015+B0015 Output (LVA tag)
Correct.JPG
Correct.JPG
K145252 R0040+E0240 Output (no tag)
Correct.JPG
Correct.JPG
K145280 K145201+K145205 Input-Output: test ligation mix
Wrong.JPG
K145279 K145201+K145252 Input-Output: test ligation mix
Wrong.JPG
K145258 R0040+B0033+C0056+B0015 Memory - part 1 ligation mix
Wrong.JPG
K145259 R0053+P0152+R1052+P0353 Memory - part 2
Wrong.JPG
Wrong.JPG
K145210 K145258+K145259 Memory
Wrong.JPG
Wrong.JPG
K145211 R1052+K145013+B0015 Memory - part 3
Wrong.JPG
Wrong.JPG
K145215 R0040+J23066 Filter - part 1
Correct.JPG
Wrong.JPG
K145278 J23109+J23032+K145001+B0015 Filter (old) - part 2 ligation mix
Wrong.JPG
K145277 J23109+J23078+K145001+B0015 Filter (new) - part 2
Wrong.JPG
Wrong.JPG
K145275 K145215+K145278 Filter (old)
Wrong.JPG
Wrong.JPG
K145276 K145215+K145277 Filter (new)
Wrong.JPG
Wrong.JPG
K145253 I712074+J23032+C0012+B0015 Invertimer (old) - part 1
Correct.JPG
Wrong.JPG
K145255 I712074+J23078+C0012+B0015 Invertimer (new) - part 1
Wrong.JPG
Wrong.JPG
K145254 R0011+B0032+C0061+B0015 Invertimer - part 2 electroporation
Wrong.JPG
K145220 K145255+K145254 Invertimer (new)
Wrong.JPG
Wrong.JPG
K145104 K145253+K145254 Invertimer (old)
Wrong.JPG
Wrong.JPG
K145251 I712074+J23032+C0060+B0015 Reset (old)
Correct.JPG
Wrong.JPG
K145225 I712074+J23078+C0060+B0015 Reset (new)
Wrong.JPG
Wrong.JPG
K145256 K145150+B0034+C0062 Cell Death - part 1
Wrong.JPG
Wrong.JPG
K145257 B0014+B0033+K145151+B0015 Cell Death - part 2 electroporation
Wrong.JPG
K145230 K145256+K145257 Cell Death
Wrong.JPG
Wrong.JPG
K145273 R0040+P0353 test K145150 - part 2 electroporation
Wrong.JPG
K145270 J23116+B0032+C0062+B0015 test K145150 - part 3 ligation mix
Wrong.JPG
K145269 K145150+E0240 test K145150 - part 4 electroporation
Wrong.JPG
K145268 K145201+K145273 test K145150 - part 5
Wrong.JPG
Wrong.JPG
K145267 K145270+K145269 test K145150 - part 6
Wrong.JPG
Wrong.JPG
K145266 K145268+K145267 test K145150
Wrong.JPG
Wrong.JPG
K145271 I712074+J23078+E0040+B0015 test filter (new) - part 3
Wrong.JPG
Wrong.JPG
K145272 I712074+J23032+E0040+B0015 test filter (old) - part 3
Wrong.JPG
Wrong.JPG
K145265 K145275+K145272 test filter (old)
Wrong.JPG
Wrong.JPG
K145264 K145276+K145271 test filter (new)
Wrong.JPG
Wrong.JPG
Retrieved from "http://2008.igem.org/Team:KULeuven/Ligation"