Team:KULeuven/Model/Output

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=== Sensitivity Analysis ===
=== Sensitivity Analysis ===
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[[Image:Sens output.png|center]]

Revision as of 12:00, 7 August 2008

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Pictogram output.png

Contents

Output

Position in the system

The output system is one of three modules directly linked to the output of the light sensor (black-box) the input for is OmpF. The output system is a simple gene regulation, of which transcription is activated by OmpF. The output signal is CFP (cyan fluorescent protein).

In the applied project of dealing with Crohn's disease this would be replaced by the drug necessary to cure the local inflammation. The drug concentration would be proportional to the amount of local inflammation sensed.

Describing the system

Output BioBrick.jpg

ODE's

Parameters

Parameter values (Output)
Name Value Comments Reference
Degradation Rates
dCFP (protein) 1.05E-4 s-1 [http://parts.mit.edu/igem07/index.php/ETHZ/Parameters link]
dRNA_CFP (mRNA) 0.0023 s-1 [http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=124983&blobtype=pdf link]
Transcription Rates
kCFP OmpF dependent
Translation Rates
kCFP 0.05 s-1 RBS is B0032 (efficiency of 0.3)

Models

CellDesigner (SBML file)

The Output model represented in CellDesigner:

Output

Several simulations were conducted, the following two show the transient respons and eventual saturation of the output system to a low and a high step signal as input, respectively. We see that there is a great distinction in the number of CFP molecules between the two input signals.

Sim output 1.png
Sim output 2.png

Next figure shows the switching behaviour of the output system to an light signal turning off and on:

Sim output 3.png

Matlab (SBML file)

The diagram for the output system is shown below:

Output Matlab.jpg

Another two simulations have been conducted, matching the solutions found by CellDesigner, the low input respons (0.0005 OmpF s-1) is on the left, the high input respons (0.025 OmpF s-1) on the right:

Sim output low input.jpg
Sim output high input.jpg

Next figure demonstrates the switching behaviour and concides with the results predicted by CellDesigner:

Sim output switch input.jpg

Sensitivity Analysis

Sens output.png