Team:KULeuven/Parts

From 2008.igem.org

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== Submitted Parts ==
 
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{| style="background:#99CCFF;color:black;width:100%;" border="0" cellpadding="10" cellspacing="0" align="center"
 
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|-
 
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! Part number
 
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! Replaced by
 
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! Description
 
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! Type
 
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! Component
 
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|- style="background:#f0f0f0; color:black"
 
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| '''K145000'''
 
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| '''K145100'''
 
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| eCFP with LVA tag under OmpR control
 
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| Part
 
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| Output
 
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|- style="background:#f0f0f0; color:black"
 
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| '''K145001'''
 
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| /
 
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| T7 RNA polymerase
 
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| Part
 
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| ''(nowhere)''
 
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|- style="background:#f0f0f0; color:black"
 
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| '''K145002'''
 
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| /
 
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| T7 RNA Polymerase under OmpF control
 
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| Composite part
 
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| ''(nowhere)''
 
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|- style="background:#f0f0f0; color:black"
 
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| '''K145003'''
 
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| '''K145102'''
 
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| RiboKey 3d under control of T7 RNA polymerase promotor
 
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| Composite part
 
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| ''(nowhere)''
 
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|- style="background:#f0f0f0; color:black"
 
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| '''K145004'''
 
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| '''K145104'''
 
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| T7 PoPS + RiboLock generate LacI Repressor. LacI Repressor represses transcription of LuxI from pLac.
 
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| Composite part
 
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| Inverter
 
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|- style="background:#f0f0f0; color:black"
 
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| '''K145005'''
 
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| /
 
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| cI under control of a dual promotor and a RiboLock.
 
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| Composite part
 
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| Pulse generator
 
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|- style="background:#f0f0f0; color:black"
 
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| '''K145006'''
 
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| '''K145106'''
 
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| Lactonase under PRM control
 
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| Composite part
 
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| ''(nowhere)''
 
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|- style="background:#f0f0f0; color:black"
 
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| '''K145007'''
 
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| '''K145107'''
 
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| Pulse Generator
 
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| Composite part
 
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| ''(nowhere)''
 
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|- style="background:#f0f0f0; color:black"
 
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| '''K145008'''
 
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| '''K145108'''
 
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| Constitutive production of LuxR
 
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| Composite part
 
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| ''(nowhere)''
 
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|- style="background:#f0f0f0; color:black"
 
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| '''K145009'''
 
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| '''K145109'''
 
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| ccdB cell death gene under control of an activating Lux PR
 
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| Composite part
 
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| ''(nowhere)''
 
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|- style="background:#f0f0f0; color:black"
 
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| '''K145010'''
 
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| '''K145110'''
 
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| Killing switch
 
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| Composite part
 
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| ''(nowhere)''
 
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|- style="background:#f0f0f0; color:black"
 
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| '''K145011'''
 
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| /
 
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| Light sensor to provide dummy input for the system
 
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| Composite part, copy of '''BBa_M30109'''
 
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| input
 
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|- style="background:#f0f0f0; color:black"
 
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| '''K145012'''
 
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| /
 
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| N-terminal degradation tag
 
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| Part
 
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| Filter
 
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|- style="background:#f0f0f0; color:black"
 
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| '''K145013'''
 
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| /
 
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| antisense LuxI
 
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| Part
 
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| Memory
 
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|-style="background:#f0f0f0; color:black"
 
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| '''K145014'''
 
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| /
 
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| T7 RNA Polymerase with N-terminal UmuD degradation tag
 
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| Part
 
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| Filter
 
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|- style="background:#f0f0f0; color:black"
 
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| '''K145015'''
 
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| /
 
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| GFP with LVA tag for rapid degradation
 
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| Part
 
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| Ouput
 
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|- style="background:#f0f0f0; color:black"
 
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| '''K145100'''
 
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| /
 
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| eCFP with LVA tag under control of a OmpF promotor
 
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| Composite part
 
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| Output
 
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|- style="background:#f0f0f0; color:black"
 
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| '''K145101'''
 
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| /
 
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| Memory device in which P2ogr is under control of OmpF and Psid double promotor. P2ogr will also activate transcription of cII from a seperate Psid promotor. This cII represses transcription of antisense LuxI from a P22 promotor.
 
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| Composite part
 
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| Memory
 
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|- style="background:#f0f0f0; color:black"
 
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| '''K145102'''
 
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| /
 
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| RiboKey3d under control of OmpF promotor
 
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| Composite part
 
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| Filter
 
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|- style="background:#f0f0f0; color:black"
 
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| '''K145103'''
 
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| /
 
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| T7 with UmuD derived tag under control of a constitutive promotor and a RiboLock3d
 
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| Composite Part
 
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| Filter
 
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|- style="background:#f0f0f0; color:black"
 
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| '''K145104'''
 
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| /
 
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| AND part, T7 and RiboKey3d together will produce LacI. This will repress transcription of LuxI from a pLac promotor
 
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| Composite part
 
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| Inverter
 
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|- style="background:#f0f0f0; color:black"
 
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| '''K145105'''
 
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| /
 
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| cI under T7 and PR dual promotor system. Should generate a pulse of cI.
 
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| Composite part
 
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| Pulse Generator
 
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|- style="background:#f0f0f0; color:black"
 
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| '''K145106'''
 
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| /
 
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| Lactonase under PRM control
 
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| Composite part
 
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| Pulse Generator
 
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|- style="background:#f0f0f0; color:black"
 
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| '''K145107'''
 
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| '''K145117'''
 
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| Generates a pulse of lactonase expression when T7 RNA polymerase AND RiboKey3d are present
 
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| Composite part
 
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| Pulse Generator
 
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|- style="background:#f0f0f0; color:black"
 
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| '''K145108'''
 
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| /
 
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| LuxR and TetR under control of a constitutive promotor.
 
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| Composite part
 
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| Cell death
 
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|- style="background:#f0f0f0; color:black"
 
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| '''K145109'''
 
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| /
 
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| ccdB cell death gene under the control of a LuxPR promotor.
 
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| Composite part
 
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| Cell death
 
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|- style="background:#f0f0f0; color:black"
 
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| '''K145110'''
 
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| /
 
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| Complete cell death mechanism. Combination of '''BBa_ K145108''' and '''BBa_K145109'''.
 
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| Composite part
 
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| Cell death
 
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|- style="background:#f0f0f0; color:black"
 
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| '''K145111'''
 
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| /
 
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| Memory device in which P2ogr is under control of OmpF and Psid double promotor. P2ogr will also activate transcription of cII from a seperate Psid promotor. This cII represses transcription of antisense LuxI from a P22 promotor
 
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| Composite part
 
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| Memory
 
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|- style="background:#f0f0f0; color:black"
 
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| '''K145112'''
 
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| /
 
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| cI under T7 and PR dual promotor system. Should generate a pulse of cI.
 
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| Composite part
 
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| Pulse generator
 
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|- style="background:#f0f0f0; color:black"
 
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| '''K145115'''
 
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| /
 
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| cI under T7 and PR dual promotor system. Should generate a pulse of cI.
 
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| Composite part
 
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| Pulse generator
 
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|- style="background:#f0f0f0; color:black"
 
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| '''K145117'''
 
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| /
 
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| Generates a pulse of lactonase expression when T7 RNA polymerase AND RiboKey3d are present
 
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| Composite part
 
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| Pulse generator
 
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|}
 
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==The Big Scheme of Used Parts==
 
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[https://static.igem.org/mediawiki/2008/6/6d/THE_BIG_SCHEME.xls The Big Scheme]
 

Latest revision as of 00:10, 27 October 2008

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