Team:KULeuven/Parts
From 2008.igem.org
(Difference between revisions)
Line 103: | Line 103: | ||
| '''K145015''' | | '''K145015''' | ||
| / | | / | ||
- | | GFP with LVA tag | + | | GFP with LVA tag |
| Part | | Part | ||
| Ouput | | Ouput | ||
+ | |- style="background:#f0f0f0; color:black" | ||
+ | | '''K145016''' | ||
+ | | / | ||
+ | | Intermediate TetR-generator | ||
+ | | ? | ||
+ | | ? | ||
|- style="background:#f0f0f0; color:black" | |- style="background:#f0f0f0; color:black" | ||
| '''K145100''' | | '''K145100''' | ||
| / | | / | ||
- | | | + | | OmpF PoPS -> GFP |
| Composite part | | Composite part | ||
| Output | | Output | ||
Line 115: | Line 121: | ||
| '''K145101''' | | '''K145101''' | ||
| / | | / | ||
- | | | + | | OmpF PoPS -> deactivate constitutive antisense LuxI |
| Composite part | | Composite part | ||
| Memory | | Memory | ||
Line 121: | Line 127: | ||
| '''K145102''' | | '''K145102''' | ||
| / | | / | ||
- | | | + | | OmpF PoPS -> RiboKey3d |
| Composite part | | Composite part | ||
| Filter | | Filter | ||
Line 127: | Line 133: | ||
| '''K145103''' | | '''K145103''' | ||
| / | | / | ||
- | | T7 with UmuD derived tag under | + | | T7 with UmuD derived tag under constitutive promotor + RiboLock3d |
| Composite Part | | Composite Part | ||
| Filter | | Filter | ||
Line 133: | Line 139: | ||
| '''K145104''' | | '''K145104''' | ||
| / | | / | ||
- | | | + | | T7 PoPS + RiboLock -> LacI -> inhibition of LuxI |
| Composite part | | Composite part | ||
| Inverter | | Inverter | ||
Line 139: | Line 145: | ||
| '''K145105''' | | '''K145105''' | ||
| / | | / | ||
- | | cI under T7 and | + | | cI under T7 and P<sub>R</sub> dual promotor |
| Composite part | | Composite part | ||
| Pulse Generator | | Pulse Generator | ||
Line 145: | Line 151: | ||
| '''K145106''' | | '''K145106''' | ||
| / | | / | ||
- | | Lactonase | + | | cI P<sub>RM</sub> PoPS -> Lactonase |
| Composite part | | Composite part | ||
| Pulse Generator | | Pulse Generator | ||
Line 151: | Line 157: | ||
| '''K145107''' | | '''K145107''' | ||
| '''K145117''' | | '''K145117''' | ||
- | | | + | | Lactonase pulse generator |
| Composite part | | Composite part | ||
| Pulse Generator | | Pulse Generator | ||
Line 157: | Line 163: | ||
| '''K145108''' | | '''K145108''' | ||
| / | | / | ||
- | | LuxR | + | | Constitutive PoPS -> LuxR |
| Composite part | | Composite part | ||
| Cell death | | Cell death | ||
Line 163: | Line 169: | ||
| '''K145109''' | | '''K145109''' | ||
| / | | / | ||
- | | ccdB | + | | Hybrid P22 c2 - luxP<sub>R</sub> PoPS -> ccdB |
| Composite part | | Composite part | ||
| Cell death | | Cell death | ||
Line 169: | Line 175: | ||
| '''K145110''' | | '''K145110''' | ||
| / | | / | ||
- | | | + | | Cell death mechanism |
| Composite part | | Composite part | ||
| Cell death | | Cell death | ||
Line 175: | Line 181: | ||
| '''K145111''' | | '''K145111''' | ||
| / | | / | ||
- | | | + | | OmpF PoPS -> deactivate constitutive antisense LuxI |
| Composite part | | Composite part | ||
| Memory | | Memory | ||
Line 181: | Line 187: | ||
| '''K145112''' | | '''K145112''' | ||
| / | | / | ||
- | | cI under T7 and PR dual promotor | + | | cI under T7 and PR<sub>R</sub> dual promotor |
| Composite part | | Composite part | ||
| Pulse generator | | Pulse generator | ||
+ | |- style="background:#f0f0f0; color:black" | ||
+ | | '''K145113''' | ||
+ | | / | ||
+ | | Constitutive PoPS -> TetR | ||
+ | | ? | ||
+ | | ? | ||
|- style="background:#f0f0f0; color:black" | |- style="background:#f0f0f0; color:black" | ||
| '''K145115''' | | '''K145115''' | ||
| / | | / | ||
- | | cI under T7 and | + | | cI under T7 and PRR dual promotor |
| Composite part | | Composite part | ||
| Pulse generator | | Pulse generator | ||
Line 193: | Line 205: | ||
| '''K145117''' | | '''K145117''' | ||
| / | | / | ||
- | | | + | | Lactonase pulse generator |
| Composite part | | Composite part | ||
| Pulse generator | | Pulse generator | ||
+ | |- style="background:#f0f0f0; color:black" | ||
+ | | '''K145150''' | ||
+ | | / | ||
+ | | Hybrid promoter: HSL-LuxR activated, P22 C2 repressed | ||
+ | | ? | ||
+ | | ? | ||
+ | |- style="background:#f0f0f0; color:black" | ||
+ | | '''K145151''' | ||
+ | | / | ||
+ | | ccdB coding region | ||
+ | | ? | ||
+ | | ? | ||
+ | |- style="background:#f0f0f0; color:black" | ||
+ | | '''K145152''' | ||
+ | | / | ||
+ | | Hybrid promoter: P22 c2 , LacI NOR gate | ||
+ | | ? | ||
+ | | ? | ||
+ | |- style="background:#f0f0f0; color:black" | ||
+ | | '''K1451''' | ||
+ | | / | ||
+ | | | ||
+ | | ? | ||
+ | | ? | ||
+ | |- style="background:#f0f0f0; color:black" | ||
+ | | '''K145200''' | ||
+ | | / | ||
+ | | INPUT lightsensor (DISCARDED) | ||
+ | | ? | ||
+ | | ? | ||
+ | |- style="background:#f0f0f0; color:black" | ||
+ | | '''K145201''' | ||
+ | | / | ||
+ | | INPUT TetR generator | ||
+ | | ? | ||
+ | | ? | ||
+ | |- style="background:#f0f0f0; color:black" | ||
+ | | '''K145205''' | ||
+ | | / | ||
+ | | OUTPUT GFP under TetR pomoter | ||
+ | | ? | ||
+ | | ? | ||
+ | |- style="background:#f0f0f0; color:black" | ||
+ | | '''K145210''' | ||
+ | | / | ||
+ | | MEMORY | ||
+ | | ? | ||
+ | | ? | ||
+ | |- style="background:#f0f0f0; color:black" | ||
+ | | '''K145211''' | ||
+ | | / | ||
+ | | MEMORY OUTPUT asLuxI | ||
+ | | ? | ||
+ | | ? | ||
+ | |- style="background:#f0f0f0; color:black" | ||
+ | | '''K145215''' | ||
+ | | / | ||
+ | | FILTER Key | ||
+ | | ? | ||
+ | | ? | ||
+ | |- style="background:#f0f0f0; color:black" | ||
+ | | '''K145216''' | ||
+ | | / | ||
+ | | FILTER T7 RNA pol | ||
+ | | ? | ||
+ | | ? | ||
+ | |- style="background:#f0f0f0; color:black" | ||
+ | | '''K145217''' | ||
+ | | / | ||
+ | | FILTER Complete | ||
+ | | ? | ||
+ | | ? | ||
+ | |- style="background:#f0f0f0; color:black" | ||
+ | | '''K145220''' | ||
+ | | / | ||
+ | | INVERTED TIMER | ||
+ | | ? | ||
+ | | ? | ||
+ | |- style="background:#f0f0f0; color:black" | ||
+ | | '''K145225''' | ||
+ | | / | ||
+ | | RESET lactonase | ||
+ | | ? | ||
+ | | ? | ||
+ | |- style="background:#f0f0f0; color:black" | ||
+ | | '''K145230''' | ||
+ | | / | ||
+ | | CELL DEATH | ||
+ | | ? | ||
+ | | ? | ||
|} | |} | ||
Revision as of 08:35, 26 August 2008
dock/undock dropdown
Submitted Parts
Part number | Replaced by | Description | Type | Component |
---|---|---|---|---|
K145000 | K145100 | eCFP with LVA tag under OmpR control | Part | Output |
K145001 | / | T7 RNA polymerase | Part | (nowhere) |
K145002 | / | T7 RNA Polymerase under OmpF control | Composite part | (nowhere) |
K145003 | K145102 | RiboKey 3d under control of T7 RNA polymerase promotor | Composite part | (nowhere) |
K145004 | K145104 | T7 PoPS + RiboLock generate LacI Repressor. LacI Repressor represses transcription of LuxI from pLac. | Composite part | Inverter |
K145005 | / | cI under control of a dual promotor and a RiboLock. | Composite part | Pulse generator |
K145006 | K145106 | Lactonase under PRM control | Composite part | (nowhere) |
K145007 | K145107 | Pulse Generator | Composite part | (nowhere) |
K145008 | K145108 | Constitutive production of LuxR | Composite part | (nowhere) |
K145009 | K145109 | ccdB cell death gene under control of an activating Lux PR | Composite part | (nowhere) |
K145010 | K145110 | Killing switch | Composite part | (nowhere) |
K145011 | / | Light sensor to provide dummy input for the system | Composite part, copy of BBa_M30109 | input |
K145012 | / | N-terminal degradation tag | Part | Filter |
K145013 | / | antisense LuxI | Part | Memory |
K145014 | / | T7 RNA Polymerase with N-terminal UmuD degradation tag | Part | Filter |
K145015 | / | GFP with LVA tag | Part | Ouput |
K145016 | / | Intermediate TetR-generator | ? | ? |
K145100 | / | OmpF PoPS -> GFP | Composite part | Output |
K145101 | / | OmpF PoPS -> deactivate constitutive antisense LuxI | Composite part | Memory |
K145102 | / | OmpF PoPS -> RiboKey3d | Composite part | Filter |
K145103 | / | T7 with UmuD derived tag under constitutive promotor + RiboLock3d | Composite Part | Filter |
K145104 | / | T7 PoPS + RiboLock -> LacI -> inhibition of LuxI | Composite part | Inverter |
K145105 | / | cI under T7 and PR dual promotor | Composite part | Pulse Generator |
K145106 | / | cI PRM PoPS -> Lactonase | Composite part | Pulse Generator |
K145107 | K145117 | Lactonase pulse generator | Composite part | Pulse Generator |
K145108 | / | Constitutive PoPS -> LuxR | Composite part | Cell death |
K145109 | / | Hybrid P22 c2 - luxPR PoPS -> ccdB | Composite part | Cell death |
K145110 | / | Cell death mechanism | Composite part | Cell death |
K145111 | / | OmpF PoPS -> deactivate constitutive antisense LuxI | Composite part | Memory |
K145112 | / | cI under T7 and PRR dual promotor | Composite part | Pulse generator |
K145113 | / | Constitutive PoPS -> TetR | ? | ? |
K145115 | / | cI under T7 and PRR dual promotor | Composite part | Pulse generator |
K145117 | / | Lactonase pulse generator | Composite part | Pulse generator |
K145150 | / | Hybrid promoter: HSL-LuxR activated, P22 C2 repressed | ? | ? |
K145151 | / | ccdB coding region | ? | ? |
K145152 | / | Hybrid promoter: P22 c2 , LacI NOR gate | ? | ? |
K1451 | / | ? | ? | |
K145200 | / | INPUT lightsensor (DISCARDED) | ? | ? |
K145201 | / | INPUT TetR generator | ? | ? |
K145205 | / | OUTPUT GFP under TetR pomoter | ? | ? |
K145210 | / | MEMORY | ? | ? |
K145211 | / | MEMORY OUTPUT asLuxI | ? | ? |
K145215 | / | FILTER Key | ? | ? |
K145216 | / | FILTER T7 RNA pol | ? | ? |
K145217 | / | FILTER Complete | ? | ? |
K145220 | / | INVERTED TIMER | ? | ? |
K145225 | / | RESET lactonase | ? | ? |
K145230 | / | CELL DEATH | ? | ? |