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Team:KULeuven/Primers
From 2008.igem.org
(Difference between revisions)
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{|cellspacing="1" width=100% | {|cellspacing="1" width=100% | ||
| - | ! style="background:#003E81; color: white" colspan=" | + | ! style="background:#003E81; color: white" colspan="6" | Table of Oligos |
|-style="background:#99CCFF" align="center" | |-style="background:#99CCFF" align="center" | ||
| width=5% | Number | | width=5% | Number | ||
| width=10% | Name | | width=10% | Name | ||
| - | | width= | + | | width=55% | Sequence |
| width=5% | Length | | width=5% | Length | ||
| + | | width=5% | Tm (°C) | ||
| width=20% | Comments | | width=20% | Comments | ||
{{:Team:KULeuven/Tools/Oligo | {{:Team:KULeuven/Tools/Oligo | ||
| Line 22: | Line 23: | ||
|sequence= <div class="code">ATGAATTCGC GGCCGCTTCT AGATGAACAC GATTAACATC GC</div> | |sequence= <div class="code">ATGAATTCGC GGCCGCTTCT AGATGAACAC GATTAACATC GC</div> | ||
|length=42bp | |length=42bp | ||
| + | |Tm=66.7 | ||
|comments=Forward T7 RNApol primer, without UmuD tag | |comments=Forward T7 RNApol primer, without UmuD tag | ||
}} | }} | ||
| Line 29: | Line 31: | ||
|sequence= <div class="code">CTGCAGCGGC CGCTACTAGT ATTATTACGC GAACGCGAAG TCCG</div> | |sequence= <div class="code">CTGCAGCGGC CGCTACTAGT ATTATTACGC GAACGCGAAG TCCG</div> | ||
|length=44bp | |length=44bp | ||
| + | |Tm=59.9 | ||
|comments=Reverse T7 RNApol primer | |comments=Reverse T7 RNApol primer | ||
}} | }} | ||
| Line 36: | Line 39: | ||
|sequence= <div class="code">CCGCTATTTA GCGATCTTGT TCAGTGTGGC TTTCCTTCAC CGATGAACAC GATTAACATC GC</div> | |sequence= <div class="code">CCGCTATTTA GCGATCTTGT TCAGTGTGGC TTTCCTTCAC CGATGAACAC GATTAACATC GC</div> | ||
|length=62bp | |length=62bp | ||
| + | |Tm=51.0 | ||
|comments=Forward T7 RNApol primer, with UmuD tag, part 1 | |comments=Forward T7 RNApol primer, with UmuD tag, part 1 | ||
}} | }} | ||
| Line 43: | Line 47: | ||
|sequence= <div class="code">ATGAATTCGC GGCCGCTTCT AGATGTTGTT TATCAAGCCT GCGGATCTCC GCGAAATTGT GACTTTTCCG CTATTTAGCG ATCTTGTTCA G</div> | |sequence= <div class="code">ATGAATTCGC GGCCGCTTCT AGATGTTGTT TATCAAGCCT GCGGATCTCC GCGAAATTGT GACTTTTCCG CTATTTAGCG ATCTTGTTCA G</div> | ||
|length=91bp | |length=91bp | ||
| + | |Tm=55.8 | ||
|comments=Forward T7 RNApol primer, with UmuD tag, part 2, very expensive | |comments=Forward T7 RNApol primer, with UmuD tag, part 2, very expensive | ||
}} | }} | ||
| Line 49: | Line 54: | ||
|name=GFP-LVA Fw | |name=GFP-LVA Fw | ||
|sequence= <div class="code">CATCATGAAT TCGCGGCCGC TTCTAGATGC GTAAAGGAGA AGAACTTTTC ACTGG</div> | |sequence= <div class="code">CATCATGAAT TCGCGGCCGC TTCTAGATGC GTAAAGGAGA AGAACTTTTC ACTGG</div> | ||
| + | |Tm=58.9 | ||
|length=55bp | |length=55bp | ||
|comments=Forward GFP-LVA primer | |comments=Forward GFP-LVA primer | ||
| Line 55: | Line 61: | ||
|nr=1085 | |nr=1085 | ||
|name=GFP-LVA Rev | |name=GFP-LVA Rev | ||
| - | |sequence= <div class="code">CTGCAGCGGC CGCTACTAGT ATTATTAAGC TACTAAAGCG TAGTTTTCGT CGTTTGCAGC AGGC | + | |sequence= <div class="code">CTGCAGCGGC CGCTACTAGT ATTATTAAGC TACTAAAGCG TAGTTTTCGT CGTTTGCAGC AGGC</div> |
|length=64bp | |length=64bp | ||
| + | |Tm=64.9 | ||
|comments=Reverse GFP-LVA primer | |comments=Reverse GFP-LVA primer | ||
}} | }} | ||
| Line 64: | Line 71: | ||
|sequence= <div class="code">TGCCACCTGA CGTCTAAGAA</div> | |sequence= <div class="code">TGCCACCTGA CGTCTAAGAA</div> | ||
|length=20bp | |length=20bp | ||
| + | |Tm=55.9 | ||
|comments=Forward primer for sequence analysis | |comments=Forward primer for sequence analysis | ||
}} | }} | ||
| Line 71: | Line 79: | ||
|sequence= <div class="code">ATTACCGCCT TTGAGTGAGC</div> | |sequence= <div class="code">ATTACCGCCT TTGAGTGAGC</div> | ||
|length=20bp | |length=20bp | ||
| + | |Tm=55.3 | ||
|comments=Reverse primer for sequence analysis | |comments=Reverse primer for sequence analysis | ||
}} | }} | ||
|} | |} | ||
Revision as of 10:57, 1 August 2008
dock/undock dropdown
| Table of Oligos | |||||
|---|---|---|---|---|---|
| Number | Name | Sequence | Length | Tm (°C) | Comments |
| 1076 | T7 Fw, no tag | ATGAATTCGC GGCCGCTTCT AGATGAACAC GATTAACATC GC
| 42bp | 66.7 | Forward T7 RNApol primer, without UmuD tag |
| 1077 | T7 Rev | CTGCAGCGGC CGCTACTAGT ATTATTACGC GAACGCGAAG TCCG
| 44bp | 59.9 | Reverse T7 RNApol primer |
| 1078 | T7 Fw, UmuD tag, part 1 | CCGCTATTTA GCGATCTTGT TCAGTGTGGC TTTCCTTCAC CGATGAACAC GATTAACATC GC
| 62bp | 51.0 | Forward T7 RNApol primer, with UmuD tag, part 1 |
| 1079 | T7 Tw, UmuD tag, part 2 | ATGAATTCGC GGCCGCTTCT AGATGTTGTT TATCAAGCCT GCGGATCTCC GCGAAATTGT GACTTTTCCG CTATTTAGCG ATCTTGTTCA G
| 91bp | 55.8 | Forward T7 RNApol primer, with UmuD tag, part 2, very expensive |
| 1084 | GFP-LVA Fw | CATCATGAAT TCGCGGCCGC TTCTAGATGC GTAAAGGAGA AGAACTTTTC ACTGG
| 55bp | 58.9 | Forward GFP-LVA primer |
| 1085 | GFP-LVA Rev | CTGCAGCGGC CGCTACTAGT ATTATTAAGC TACTAAAGCG TAGTTTTCGT CGTTTGCAGC AGGC
| 64bp | 64.9 | Reverse GFP-LVA primer |
| 1074 | VF2 | TGCCACCTGA CGTCTAAGAA
| 20bp | 55.9 | Forward primer for sequence analysis |
| 1075 | VR | ATTACCGCCT TTGAGTGAGC
| 20bp | 55.3 | Reverse primer for sequence analysis |
