Team:KULeuven/Primers

From 2008.igem.org

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! style="background:#003E81; color: white" colspan="5" | Table of Oligos
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{|cellspacing="1"; width="85%"
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! style="background:#003E81; color: white" colspan="6" align="center" height="30px"| '''Table of Oligos'''
|-style="background:#99CCFF" align="center"
|-style="background:#99CCFF" align="center"
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| width=5% | Number
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| width=5% | '''Number'''
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| width=10% | Name
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| width=10% | '''Name'''
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| width=60% | Sequence
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| width=35% | '''Sequence'''
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| width=5% | Length
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| width=5% | '''Length'''
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| width=20% | Comments
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| width=5% | '''Tm (°C)'''
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| width=25% | '''Comments'''
{{:Team:KULeuven/Tools/Oligo
{{:Team:KULeuven/Tools/Oligo
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|nr=1
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|nr=1076
|name=T7 Fw, no tag
|name=T7 Fw, no tag
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|sequence=catcatgaattcgcggccgcttctagatgaacacgattaacatcgc
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|sequence= <div class="code">ATGAATTCGC GGCCGCTTCT AGATGAACAC GATTAACATC GC</div>
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|length=46bp
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|length=42bp
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|Tm=66.7
|comments=Forward T7 RNApol primer, without UmuD tag
|comments=Forward T7 RNApol primer, without UmuD tag
}}
}}
{{:Team:KULeuven/Tools/Oligo
{{:Team:KULeuven/Tools/Oligo
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|nr=2
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|nr=1077
|name=T7 Rev
|name=T7 Rev
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|sequence=ctgcagcggccgctactagtattattacgcgaacgcgaagtccg
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|sequence= <div class="code">CTGCAGCGGC CGCTACTAGT ATTATTACGC GAACGCGAAG TCCG</div>
|length=44bp
|length=44bp
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|Tm=59.9
|comments=Reverse T7 RNApol primer
|comments=Reverse T7 RNApol primer
}}
}}
{{:Team:KULeuven/Tools/Oligo
{{:Team:KULeuven/Tools/Oligo
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|nr=3
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|nr=1078
|name=T7 Fw, UmuD tag, part 1
|name=T7 Fw, UmuD tag, part 1
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|sequence=ccgctatttagcgatcttgttcagtgtggctttccttcaccgatgaacacgattaacatcgc
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|sequence= <div class="code">CCGCTATTTA GCGATCTTGT TCAGTGTGGC TTTCCTTCAC CGATGAACAC GATTAACATC GC</div>
|length=62bp
|length=62bp
 +
|Tm=51.0
|comments=Forward T7 RNApol primer, with UmuD tag, part 1
|comments=Forward T7 RNApol primer, with UmuD tag, part 1
}}
}}
{{:Team:KULeuven/Tools/Oligo
{{:Team:KULeuven/Tools/Oligo
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|nr=4
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|nr=1079
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|name=T7 Tw, UmuD tag, part 2
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|name=T7 Fw, UmuD tag, part 2
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|sequence=catcatgaattcgcggccgcttctagatgttgtttatcaagcctgcggatctccgcgaaattgtgacttttccgctatttagcgatcttgttcag
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|sequence= <div class="code">ATGAATTCGC GGCCGCTTCT AGATGTTGTT TATCAAGCCT GCGGATCTCC GCGAAATTGT GACTTTTCCG CTATTTAGCG ATCTTGTTCA G</div>
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|length=95bp
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|length=91bp
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|Tm=55.8
|comments=Forward T7 RNApol primer, with UmuD tag, part 2, very expensive
|comments=Forward T7 RNApol primer, with UmuD tag, part 2, very expensive
}}
}}
{{:Team:KULeuven/Tools/Oligo
{{:Team:KULeuven/Tools/Oligo
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|nr=5
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|nr=1084
|name=GFP-LVA Fw
|name=GFP-LVA Fw
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|sequence=catcatgaattcgcggccgcttctagatgcgtaaaggagaagaacttttcactgg
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|sequence= <div class="code">CATCATGAAT TCGCGGCCGC TTCTAGATGC GTAAAGGAGA AGAACTTTTC ACTGG</div>
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|Tm=58.9
|length=55bp
|length=55bp
|comments=Forward GFP-LVA primer
|comments=Forward GFP-LVA primer
}}
}}
{{:Team:KULeuven/Tools/Oligo
{{:Team:KULeuven/Tools/Oligo
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|nr=6
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|nr=1085
|name=GFP-LVA Rev
|name=GFP-LVA Rev
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|sequence=ctgcagcggccgctactagtattattaagctactaaagcgtagttttcgtcgtttgcagcaggc
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|sequence= <div class="code">CTGCAGCGGC CGCTACTAGT ATTATTAAGC TACTAAAGCG TAGTTTTCGT CGTTTGCAGC AGGC</div>
|length=64bp
|length=64bp
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|Tm=64.9
|comments=Reverse GFP-LVA primer
|comments=Reverse GFP-LVA primer
}}
}}
{{:Team:KULeuven/Tools/Oligo
{{:Team:KULeuven/Tools/Oligo
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|nr=7
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|nr=1074
|name=VF2
|name=VF2
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|sequence=TGCCACCTGACGTCTAAGAA
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|sequence= <div class="code">TGCCACCTGA CGTCTAAGAA</div>
|length=20bp
|length=20bp
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|Tm=55.9
|comments=Forward primer for sequence analysis
|comments=Forward primer for sequence analysis
}}
}}
{{:Team:KULeuven/Tools/Oligo
{{:Team:KULeuven/Tools/Oligo
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|nr=8
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|nr=1075
|name=VR
|name=VR
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|sequence=ATTACCGCCTTTGAGTGAGC
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|sequence= <div class="code">ATTACCGCCT TTGAGTGAGC</div>
|length=20bp
|length=20bp
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|Tm=55.3
|comments=Reverse primer for sequence analysis
|comments=Reverse primer for sequence analysis
 +
}}
 +
{{:Team:KULeuven/Tools/Oligo
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|nr=1100
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|name=antisense luxI Fw
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|sequence= <div class="code">CATCAGGAAT TCGCGGCCGC TTCTAGTTAT TAAGCTACTA AAGCGTAGTT TTC</div>
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|length=53bp
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|Tm=51.6
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|comments=Forward primer for construction of antisense luxI, contains prefix, contains additional <div class="code">CATCAG</div>
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}}
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{{:Team:KULeuven/Tools/Oligo
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|nr=1101
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|name=antisense luxI Rev
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|sequence= <div class="code">CTGCAGCGGC CGCTACTAGT AATGACTATA ATGATAAAAA AATCGG</div>
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|length=46bp
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|Tm=47.8
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|comments=Reverse primer for construction of antisense luxI, contains suffix, 25 bp annealing
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}}
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{{:Team:KULeuven/Tools/Oligo
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|nr=1127
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|name=''ccdB'' coding Fw
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|sequence=<div class="code">CATCATGAAT TCGCGGCCGC TTCTAGATGC AGTTTAAGGT TTACACC</div>
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|Tm=50.9
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|length=47
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|comments=Forward primer for amplification of ''ccdB'' coding sequence, contains prefix, additional <div class="code">CATCAT</div>
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}}
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{{:Team:KULeuven/Tools/Oligo
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|nr=1128
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|name=''ccdB'' coding Rev
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|sequence=<div class="code">CTGCAGCGGC CGCTACTAGT ATTATATTCC CCAGAACATC AGG</div>
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|Tm=51.8
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|length=43
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|comments=Reverse primer for amplification of ''ccdB'', contains suffix
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}}
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{{:Team:KULeuven/Tools/Oligo
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|nr=1129
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|name=Hybrid promoter left linker
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|sequence=<div class="code">CATCATGAAT TCGCGGCCGC TTCTAGACCT GTAGGATCGT ACAGGTTTAC TAAAGATTCC TTTAG</div>
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|Tm='''check'''
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|length=65
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|comments=Left linker for construction of hybrid promoter by end filling, repressed by P22 c2 (output of memory in thr OFF state) and activated by HSL_LuxR, 16 bp overlap, contains prefix, additional <div class="code">CATCAT</div>
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}}
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{{:Team:KULeuven/Tools/Oligo
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|nr=1130
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|name=Hybrid promoter right linker
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|sequence=<div class="code">TACTAGTAGC GGCCGCTGCA GGAAATTAAA GAACACTTAA ATTATAAACT AAAGGAATCT TTAG</div>
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|Tm='''check'''
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|length=64
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|comments=right linker for construction of hybrid promoter by end filling, repressed by P22 c2 (output of memory in thr OFF state) and activated by HSL_LuxR, 16 bp overlap, contains suffix
 +
}}
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{{:Team:KULeuven/Tools/Oligo
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|nr=1131
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|name='''BBa_I714070''' lock Fw
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|sequence=<div class="code">CATCATGAAT TCGCGGCCGC TTCTAGAGTG TAGACCGAAC TAGAATCACC TC</div>
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|Tm=58.7
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|length=52
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|comments=Forward primer for amplication of lock from '''BBa_I714070''' (J23078), contains scar, contains prefix, additional <div class="code">CATCAT</div>
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}}
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{{:Team:KULeuven/Tools/Oligo
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|nr=1132
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|name='''BBa_I714070''' lock Rev
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|sequence=<div class="code">CTGCAGCGGC CGCTACTAGT ATC</div>
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|Tm=61.5
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|length=23
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|comments=Reverse primer for amplication of lock from '''BBa_I714070''' (J23078), contains suffix
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}}
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{{:Team:KULeuven/Tools/Oligo
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|nr=1133
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|name='''BBa_I714074''' key Fw
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|sequence=<div class="code">CATCATGAAT TCGCGGCCGC TTCTAGAGAC CCAAAAGCAA GAGGTG</div>
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|Tm=57.3
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|length=46
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|comments=Forward primer for amplication of key from part '''BBa_I714074''' - J23066_B0015 (J23008, J23009, B0010, B0012), contains prefix, additional <div class="code">CATCAT</div>
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}}
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{{:Team:KULeuven/Tools/Oligo
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|nr=1134
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|name='''BBa_I714074''' key Rev
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|sequence=<div class="code">CTGCAGCGGC CGCTACTAGT ATATAAAC</div>
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|Tm=60.3
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|length=28
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|comments=Reverse primer for amplication of key from part '''BBa_I714074''' - J23066_B0015 (J23008, J23009, B0010, B0012), contains suffix
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}}
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{{:Team:KULeuven/Tools/Oligo
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|nr=1152
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|name=<div class="code">CATCAT</div>-Prefix
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|sequence=<div class="code">CATCATGAAT TCGCGGCCGC TTC</div>
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|Tm=61.2
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|length=23
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|comments=Fwd primer to anneal to the Prefix. Orginally made for the PCR after end-filling.
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}}
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{{:Team:KULeuven/Tools/Oligo
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|nr=1153
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|name=Suffix
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|sequence=<div class="code">CTGCAGCGGC CGCTACTAG</div>
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|Tm=60.9
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|length=19
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|comments=Rev primer to anneal to the Suffix. Orginally made for the PCR after end-filling.
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}}
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{{:Team:KULeuven/Tools/Oligo
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|nr=1156
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|name=T7 Fw, UmuD tag, part 2.1
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|sequence=<div class="code">CAAGCCTGCG GATCTCCGCG AAATTGTGAC TTTTCCGCTA TTTAGCGATC TTGTTCAG</div>
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|Tm=55.9
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|length=58
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|comments=Forward T7 RNApol primer, with UmuD tag, part 2.1
 +
}}
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{{:Team:KULeuven/Tools/Oligo
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|nr=1157
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|name=T7 Fw, UmuD tag, part 2.2
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|sequence=<div class="code">CATCATGAAT TCGCGGCCGC TTCTAGATGT TGTTTATCAA GCCTGCGGAT CTCC</div>
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|Tm=56.0
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|length=54
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|comments=Forward T7 RNApol primer, with UmuD tag, part 2.2
}}
}}
|}
|}

Latest revision as of 00:43, 27 October 2008

  dock/undock dropdown  



Table of Oligos
Number Name Sequence Length Tm (°C) Comments
1076 T7 Fw, no tag
ATGAATTCGC GGCCGCTTCT AGATGAACAC GATTAACATC GC
42bp 66.7 Forward T7 RNApol primer, without UmuD tag
1077 T7 Rev
CTGCAGCGGC CGCTACTAGT ATTATTACGC GAACGCGAAG TCCG
44bp 59.9 Reverse T7 RNApol primer
1078 T7 Fw, UmuD tag, part 1
CCGCTATTTA GCGATCTTGT TCAGTGTGGC TTTCCTTCAC CGATGAACAC GATTAACATC GC
62bp 51.0 Forward T7 RNApol primer, with UmuD tag, part 1
1079 T7 Fw, UmuD tag, part 2
ATGAATTCGC GGCCGCTTCT AGATGTTGTT TATCAAGCCT GCGGATCTCC GCGAAATTGT GACTTTTCCG CTATTTAGCG ATCTTGTTCA G
91bp 55.8 Forward T7 RNApol primer, with UmuD tag, part 2, very expensive
1084 GFP-LVA Fw
CATCATGAAT TCGCGGCCGC TTCTAGATGC GTAAAGGAGA AGAACTTTTC ACTGG
55bp 58.9 Forward GFP-LVA primer
1085 GFP-LVA Rev
CTGCAGCGGC CGCTACTAGT ATTATTAAGC TACTAAAGCG TAGTTTTCGT CGTTTGCAGC AGGC
64bp 64.9 Reverse GFP-LVA primer
1074 VF2
TGCCACCTGA CGTCTAAGAA
20bp 55.9 Forward primer for sequence analysis
1075 VR
ATTACCGCCT TTGAGTGAGC
20bp 55.3 Reverse primer for sequence analysis
1100 antisense luxI Fw
CATCAGGAAT TCGCGGCCGC TTCTAGTTAT TAAGCTACTA AAGCGTAGTT TTC
53bp 51.6 Forward primer for construction of antisense luxI, contains prefix, contains additional
CATCAG
1101 antisense luxI Rev
CTGCAGCGGC CGCTACTAGT AATGACTATA ATGATAAAAA AATCGG
46bp 47.8 Reverse primer for construction of antisense luxI, contains suffix, 25 bp annealing
1127 ccdB coding Fw
CATCATGAAT TCGCGGCCGC TTCTAGATGC AGTTTAAGGT TTACACC
47 50.9 Forward primer for amplification of ccdB coding sequence, contains prefix, additional
CATCAT
1128 ccdB coding Rev
CTGCAGCGGC CGCTACTAGT ATTATATTCC CCAGAACATC AGG
43 51.8 Reverse primer for amplification of ccdB, contains suffix
1129 Hybrid promoter left linker
CATCATGAAT TCGCGGCCGC TTCTAGACCT GTAGGATCGT ACAGGTTTAC TAAAGATTCC TTTAG
65 check Left linker for construction of hybrid promoter by end filling, repressed by P22 c2 (output of memory in thr OFF state) and activated by HSL_LuxR, 16 bp overlap, contains prefix, additional
CATCAT
1130 Hybrid promoter right linker
TACTAGTAGC GGCCGCTGCA GGAAATTAAA GAACACTTAA ATTATAAACT AAAGGAATCT TTAG
64 check right linker for construction of hybrid promoter by end filling, repressed by P22 c2 (output of memory in thr OFF state) and activated by HSL_LuxR, 16 bp overlap, contains suffix
1131 BBa_I714070 lock Fw
CATCATGAAT TCGCGGCCGC TTCTAGAGTG TAGACCGAAC TAGAATCACC TC
52 58.7 Forward primer for amplication of lock from BBa_I714070 (J23078), contains scar, contains prefix, additional
CATCAT
1132 BBa_I714070 lock Rev
CTGCAGCGGC CGCTACTAGT ATC
23 61.5 Reverse primer for amplication of lock from BBa_I714070 (J23078), contains suffix
1133 BBa_I714074 key Fw
CATCATGAAT TCGCGGCCGC TTCTAGAGAC CCAAAAGCAA GAGGTG
46 57.3 Forward primer for amplication of key from part BBa_I714074 - J23066_B0015 (J23008, J23009, B0010, B0012), contains prefix, additional
CATCAT
1134 BBa_I714074 key Rev
CTGCAGCGGC CGCTACTAGT ATATAAAC
28 60.3 Reverse primer for amplication of key from part BBa_I714074 - J23066_B0015 (J23008, J23009, B0010, B0012), contains suffix
1152
CATCAT
-Prefix
CATCATGAAT TCGCGGCCGC TTC
23 61.2 Fwd primer to anneal to the Prefix. Orginally made for the PCR after end-filling.
1153 Suffix
CTGCAGCGGC CGCTACTAG
19 60.9 Rev primer to anneal to the Suffix. Orginally made for the PCR after end-filling.
1156 T7 Fw, UmuD tag, part 2.1
CAAGCCTGCG GATCTCCGCG AAATTGTGAC TTTTCCGCTA TTTAGCGATC TTGTTCAG
58 55.9 Forward T7 RNApol primer, with UmuD tag, part 2.1
1157 T7 Fw, UmuD tag, part 2.2
CATCATGAAT TCGCGGCCGC TTCTAGATGT TGTTTATCAA GCCTGCGGAT CTCC
54 56.0 Forward T7 RNApol primer, with UmuD tag, part 2.2
Retrieved from "http://2008.igem.org/Team:KULeuven/Primers"