Team:KULeuven/Project/CellDeath

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(Components)
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===Components===
===Components===
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The luxR protein [http://partsregistry.org/Part:BBa_C0062 '''BBa_C0062''']  is placed under control of the constitutive promoter [http://partsregistry.org/Part:BBa_J23100 '''BBa_J23100''']. The ''ccdB'' suicide gene [http://partsregistry.org/Part:BBa_P1010 '''BBa_P1010'''], on its part, is placed under control of the LuxpR promoter [http://partsregistry.org/Part:BBa_R0062 '''BBa_R0062'''].
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The luxR protein [http://partsregistry.org/Part:BBa_C0062 '''BBa_C0062''']  is placed under control of a P22 CII hybrid promoter [http://partsregistry.org/Part:BBa_K145150 '''BBa_145150''']. The ''ccdB'' suicide gene [http://partsregistry.org/Part:BBa_P1010 '''BBa_P1010'''], on its part, is placed under control of the Luxp<sub>R</sub> promoter [http://partsregistry.org/Part:BBa_R0062 '''BBa_R0062'''].
===Action===
===Action===

Revision as of 12:05, 27 August 2008

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Pictogram celldeath.png

Contents

Cell Death

BioBricks

Project death.jpg

Components

The luxR protein BBa_C0062 is placed under control of a P22 CII hybrid promoter BBa_145150. The ccdB suicide gene BBa_P1010, on its part, is placed under control of the LuxpR promoter BBa_R0062.

Action

When the concentration of HSL is big enough (see the modeling page), HSL will form a complex with the LuxR protein and thus activate the LuxpR promoter. This results in ccdB production and eventually in cell death (reference).