"
Team:KULeuven/Project/CellDeath
From 2008.igem.org
(Difference between revisions)
| Line 5: | Line 5: | ||
==Cell Death== | ==Cell Death== | ||
| + | ===BioBricks=== | ||
'''FIGUUR''' | '''FIGUUR''' | ||
| - | The luxR protein '''BBa_C0062''' is placed under the constitutive promoter '''BBa_J23100'''. When the concentration HSL is big enough ( | + | ===Components=== |
| + | The luxR protein [http://partsregistry.org/Part:BBa_C0062 '''BBa_C0062'''] is placed under control of the constitutive promoter [http://partsregistry.org/Part:BBa_J23100 '''BBa_J23100''']. The ''ccdB'' suicide gene [http://partsregistry.org/Part:BBa_P1010 '''BBa_P1010'''], on its part, is placed under control of the LuxpR promoter [http://partsregistry.org/Part:BBa_R0062 '''BBa_R0062''']. | ||
| + | |||
| + | ===Action=== | ||
| + | When the concentration HSL is big enough (see the [https://2008.igem.org/Team:KULeuven/Model/CellDeath#CellDesigner_.28SBML_file.29 modeling page]), it will form a complex with the LuxR protein and thus activate the LuxpR promoter. This results in ''ccdB'' production and eventually cell death ([http://www.ncbi.nlm.nih.gov/pubmed/10196173?dopt=Abstract reference]). | ||
Revision as of 13:21, 31 July 2008
dock/undock dropdown
Contents |
Cell Death
BioBricks
FIGUUR
Components
The luxR protein BBa_C0062 is placed under control of the constitutive promoter BBa_J23100. The ccdB suicide gene BBa_P1010, on its part, is placed under control of the LuxpR promoter BBa_R0062.
Action
When the concentration HSL is big enough (see the modeling page), it will form a complex with the LuxR protein and thus activate the LuxpR promoter. This results in ccdB production and eventually cell death (reference).
