Team:KULeuven/Project/Reset

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This device was conceived to reset the [https://2008.igem.org/Team:KULeuven/Project/Inverter '''Timer'''].
This device was conceived to reset the [https://2008.igem.org/Team:KULeuven/Project/Inverter '''Timer'''].
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When significant input (re-)emerges, lactonase is produced.  It will then convert all available intracellular 3OC6HSL at that time to a hydroxy-acid, thereby inactivating it and efficiently removing it from the active system. In this way, the timer is reset and the cell may live to see another day.
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When significant input (re-)emerges, lactonase is produced.  It will then convert all available intracellular 3OC6HSL at that time to a hydroxy-acid, thereby inactivating it and efficiently removing it from the active system. In this way, the timer is reset and the cell may live to see another day.
Originally, a pulse of lactonase was thought to do the job, but modeling proved that the amount of lactonase produced in that system was not enough. In the simplified version described here, the amount of lactonase produced should be high enough to provide a thorough reset of our system.
Originally, a pulse of lactonase was thought to do the job, but modeling proved that the amount of lactonase produced in that system was not enough. In the simplified version described here, the amount of lactonase produced should be high enough to provide a thorough reset of our system.

Revision as of 10:36, 2 September 2008

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Logo reset.jpg

Contents

Reset

BioBricks

Lactonase.png
Project reset.jpg

Components

The system is dependent on the output of the filter, and has therefore got a T7 RNA polymerase promoter (BBa_I712074) followed by a RiboLock (BBa_J23078). The aiiA gene (BBa_C0060), short for autoinducer inactivation, is placed downstream of this AND-gate and codes for lactonase, an enzyme that hydrolyses the 3OC6HSL ester bonds. (reference)

Action

This device was conceived to reset the Timer.

When significant input (re-)emerges, lactonase is produced. It will then convert all available intracellular 3OC6HSL at that time to a hydroxy-acid, thereby inactivating it and efficiently removing it from the active system. In this way, the timer is reset and the cell may live to see another day.

Originally, a pulse of lactonase was thought to do the job, but modeling proved that the amount of lactonase produced in that system was not enough. In the simplified version described here, the amount of lactonase produced should be high enough to provide a thorough reset of our system.




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