Team:KULeuven/Project/Reset

From 2008.igem.org

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==Reset==
==Reset==
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===Action===
===Action===
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This device was conceived to reset the [https://2008.igem.org/Team:KULeuven/Project/Inverter '''Timer'''].
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This device was conceived to reset the [https://2008.igem.org/Team:KULeuven/Project/Inverter Timer].
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When significant input (re-)emerges, lactonase is produced.  It will then convert all available intracellular 3OC6HSL at that time to a hydroxy-acid, thereby inactivating it and efficiently removing it from the active system. In this way, the timer is reset and the cell may live to see another day.
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Originally, a pulse of lactonase was thought to do the job, but modeling proved that the amount of lactonase produced in that system was not enough. In the simplified version described here, the amount of lactonase produced should be high enough to provide a thorough reset of our system.
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When significant input (re-)emerges, lactonase is produced. It will then convert all available intracellular 3OC6HSL at that time to a hydroxy-acid, thereby inactivating and efficiently removing it from the active system. This way, the timer is reset and the cell may live to see another day.
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Originally, a device generating a pulse of lactonase was thought to do the job, but modeling suggested that the amount of lactonase produced during this pulse was too small to accomplish a thorough reset of our system. So the device was reinvented and a simplified (non-pulse) version was devised and described here. Now the amount of lactonase produced should be high enough for the reset to function properly.
{{:Team:KULeuven/Tools/Components}}
{{:Team:KULeuven/Tools/Components}}

Latest revision as of 14:51, 3 October 2008

  dock/undock dropdown  

Logo reset.jpg

Contents

Reset

BioBricks

Lactonase.png
Project reset.jpg

Components

The system is dependent on the output of the filter, and has therefore got a T7 RNA polymerase promoter (BBa_I712074) followed by a RiboLock (BBa_J23078). The aiiA gene (BBa_C0060), short for autoinducer inactivation, is placed downstream of this AND-gate and codes for lactonase, an enzyme that hydrolyses the 3OC6HSL ester bonds. (reference)

Action

This device was conceived to reset the Timer.

When significant input (re-)emerges, lactonase is produced. It will then convert all available intracellular 3OC6HSL at that time to a hydroxy-acid, thereby inactivating and efficiently removing it from the active system. This way, the timer is reset and the cell may live to see another day.

Originally, a device generating a pulse of lactonase was thought to do the job, but modeling suggested that the amount of lactonase produced during this pulse was too small to accomplish a thorough reset of our system. So the device was reinvented and a simplified (non-pulse) version was devised and described here. Now the amount of lactonase produced should be high enough for the reset to function properly.



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