Team:KULeuven/Project/Reset
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<div style="float: right;">[[Image:pictogram_reset.png|120px]]</div> | <div style="float: right;">[[Image:pictogram_reset.png|120px]]</div> | ||
==Reset== | ==Reset== | ||
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===BioBricks=== | ===BioBricks=== | ||
+ | <div style="float:right">[[Image:lactonase.png|330px]]</div> | ||
[[Image:project_reset.jpg|center]] | [[Image:project_reset.jpg|center]] | ||
===Components=== | ===Components=== | ||
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The system is dependent on the output of the filter, and has therefor a T7 RNA polymerase promoter ([http://partsregistry.org/Part:BBa_I712074 '''BBa_I712074''']) and RiboLock3d ([http://partsregistry.org/Part:BBa_J23032 '''BBa_J23032''']). The ''aiiA'' gene, encoded by [http://partsregistry.org/Part:BBa_C0060 '''BBa_C0060'''], produces lactonase, an enzyme that hydrolyses the 3OC6HSL ester bonds ([http://www.nature.com/nature/journal/v411/n6839/full/411813a0.html reference]). | The system is dependent on the output of the filter, and has therefor a T7 RNA polymerase promoter ([http://partsregistry.org/Part:BBa_I712074 '''BBa_I712074''']) and RiboLock3d ([http://partsregistry.org/Part:BBa_J23032 '''BBa_J23032''']). The ''aiiA'' gene, encoded by [http://partsregistry.org/Part:BBa_C0060 '''BBa_C0060'''], produces lactonase, an enzyme that hydrolyses the 3OC6HSL ester bonds ([http://www.nature.com/nature/journal/v411/n6839/full/411813a0.html reference]). | ||
Revision as of 20:44, 31 July 2008
Contents |
Reset
BioBricks
Components
The system is dependent on the output of the filter, and has therefor a T7 RNA polymerase promoter (BBa_I712074) and RiboLock3d (BBa_J23032). The aiiA gene, encoded by BBa_C0060, produces lactonase, an enzyme that hydrolyses the 3OC6HSL ester bonds (reference).
Action
The reset mechanism is made to destroy all HSL at the time, when significant input re-emerges. Originally, a pulse of lactonase was thought to do the thing, but modeling proved that the amount of lactonase produced in that system was not enough. In the simplified version described here, the concentration should be high enough and the device should work.
The aiiA gene codes for lactonase is regulated by the output signal from the filter, so that background input signal does not result in timer reset.