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Team:Paris/Modeling/f4
From 2008.igem.org
We have [FlhDC] = {coefflhDC}expr(pTet) = {coefflhDC} ƒ1([aTc]i)
and [FliA] = {coefFliA}expr(pBad) = {coefFliA} ƒ2([arab]i)
So, at steady-states,
| param | signification | unit | value | comments |
| [expr(pFlhDC)] | expression rate of pFlhDC with RBS E0032 | nM.min-1 | need for 20 mesures with well choosen values of [aTc]i and for 20 mesures with well choosen values of [arab]i and 5x5 measures for the relation below? | |
| γGFP | dilution-degradation rate of GFP(mut3b) | min-1 | 0.0198 | |
| [GFP] | GFP concentration at steady-state | nM | need for 20 + 20 measures and 5x5 measures for the relation below? | |
| (fluorescence) | value of the observed fluorescence | au | need for 20 + 20 measures and 5x5 measures for the relation below? | |
| conversion | conversion ratio between fluorescence and concentration | nM.au-1 | (1/79.429) |
| param | signification corresponding parameters in the equations | unit | value | comments |
| β5 | production rate of FlhDC-pFliA with RBS E0032 β5 | nM.min-1 | ||
| (K3/{coeffliA}) | activation constant of FlhDC-pFliA K3 | nM | ||
| n3 | complexation order of FlhDC-pFliA n3 | no dimension | ||
| β14 | production rate of FliA-pFliA with RBS E0032 β14 | nM.min-1 | ||
| (K10/{coefomp}) | activation constant of FliA-pFliA K10 | nM | ||
| n10 | complexation order of FliA-pFliA n10 | no dimension |
Then, if we have time, we want to verify the expected relation
