Team:Paris/Notebook/Oligo

From 2008.igem.org

(Difference between revisions)
(→Designed Oligonucleotides)
(→Designed Oligonucleotides)
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| style="background: #D4E2EF;" | O127
| style="background: #D4E2EF;" | O127
-
| XX Gene-EnvZ-R
+
| Gene-EnvZ-R-TRO
-
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTACCCTTCTTTTGTCGTGCCCTGCGCC
+
| GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACCCTTCTTTTGTCGTGCCCTGCGCC
-
| 59
+
| 60
-
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+
| 68°C
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|- style="background: #dddddd;"
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|- style="background: #dddddd;"
|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O129
| style="background: #D4E2EF;" | O129
-
| XX Gene-FlgM-R
+
| Gene-FlgM-R-TRO
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAGTTACTCTGCAAGTCTTGCTGCGCT
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAGTTACTCTGCAAGTCTTGCTGCGCT
| 59
| 59
-
|
+
| 59°C
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|
|- style="background: #dddddd;"
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|- style="background: #dddddd;"
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| style="background: #D4E2EF;" | O131
| style="background: #D4E2EF;" | O131
-
| XX Gene-FlhC-R
+
| Gene-FlhC-R-TRO
-
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAAACAGCCTGTACTCTCTGTTCATCC
+
| GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAAACAGCCTGTACTCTCTGTTCATCC
-
| 59
+
| 60
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| 60°C
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|- style="background: #dddddd;"
|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O133
| style="background: #D4E2EF;" | O133
-
| XX Gene-FlhD-R
+
| Gene-FlhD-R-TRO
-
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAGGCCCTTTTCTTGCGCAGCGCTTCT
+
| GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGCCCTTTTCTTGCGCAGCGCTTCT
-
| 59
+
| 60
-
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| 68°C
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|- style="background: #dddddd;"
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|- style="background: #dddddd;"
|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O135
| style="background: #D4E2EF;" | O135
-
| xx deux ecoR1 Gene-FliA-F
+
| xx Gene-FliA-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAATTCACTCTATACCGCTGAAG
| GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAATTCACTCTATACCGCTGAAG
| 53
| 53
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|- style="background: #dddddd;"
|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O137
| style="background: #D4E2EF;" | O137
-
| Gene-FliArbs-F
+
| xx Gene-FliArbs-F
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTAATCATGCCGATAACTCATATAAC
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTAATCATGCCGATAACTCATATAAC
| 53
| 53
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|- style="background: #dddddd;"
|- style="background: #dddddd;"
| style="background: #D4E2EF;" | O139
| style="background: #D4E2EF;" | O139
-
| xx Gene-OmpR-R
+
| Gene-OmpR-R
-
| GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTATGCTTTAGAGCCGTCCGGTACAAAG
+
| GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATGCTTTAGAGCCGTCCGGTACAAAG
-
| 59
+
| 60
-
|
+
| 62°C
|
|
|- style="background: #dddddd;"
|- style="background: #dddddd;"

Revision as of 16:02, 11 August 2008

Designed Oligonucleotides

Table of Oligos
Number Name Sequence Length Tm(°C) Comments
O100 FlgA-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCATATCTCCTCCGCAGGTATCAAAAT 58
O101 FlgA-R GTTTCTTCCTGCAGCGGCCGCTACTAGTAACAGTATCGCGATGATCGCCACGCTACGT 58
O102 FlgB-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGACAGTATCGCGATGATCGCCACGCTACG 58
O103 FlgB-R GTTTCTTCCTGCAGCGGCCGCTACTAGTAAGCATATCTCCTCCGCAGGTATCAAAATT 58
O104 FlgK-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCAGCAGATGAAATCGATAAGCGACAAGG 58
O105 FlgK-R GTTTCTTCCTGCAGCGGCCGCTACTAGTAAACGCCGCCTGGGCCGCGTTCAGTCCGCT 58
O106 FlgM-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGCGGCGTTGTTGATGCAGATGGGAATA 58
O107 FlgM-R GTTTCTTCCTGCAGCGGCCGCTACTAGTACGAAGTGCGATCAATACTCATGGTTTATT 58
O108 FlhB-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCACGTCATATCAGGCGGTCTGATAAGG 58
O109 FlhB-R GTTTCTTCCTGCAGCGGCCGCTACTAGTAGTTTTGTCGTCGCTCTCGTCAGACACGTC 58
O110 FlhDC-Uri-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTTGTATGTGCGTGTAGTGACGAGTACAG 58 Don't amplify the both OmpR binding site
O111 FlhDC-Total-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGTCATTTTTGCTTGCTAGCGTACGGAAAA 58 Amplify the both OmpR binding site
O112 FlhDC(rbs)-R GTTTCTTCCTGCAGCGGCCGCTACTAGTATCCCACCCAGAATAACCAACTTTAT 54 Amplify the natural rbs of FlhD
O113 FlhDC(nu)-R GTTTCTTCCTGCAGCGGCCGCTACTAGTACAGAATAACCAACTTTATTTTTATG 54 Don't amplify the natural rbs of FlhD (only promoter)
O114 xx FliA-PCR1-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCTGATTAACTGAGACTGACGGCAACGC 58
O115 xx FliA-PCR1-R CAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCCGCAGGGTTTTTAATCG 59
O116 xx FliA-PCR2-F GAAACGGATAATCATGCCGATAACTCATATAACGCAGGGCTG 42
O117 xx FliA-PCR2-R GTTTCTTCCTGCAGCGGCCGCTACTAGTACCATTACACCTTCA

GCGGTATAGAGTGAACTCACGATAAA

69
O118 FliC-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCCAGCGATGAAATACTTGCCATGCGATT 58
O119 FliC-R GTTTCTTCCTGCAGCGGCCGCTACTAGTACAGCGAGAGGCTGTTGGTATTAATGACTT 58
O120 FliE-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGGTCGCATTCATCGCGCACCTCGTGGCTG 58
O121 FliE-R GTTTCTTCCTGCAGCGGCCGCTACTAGTACTGGCTGATAACCCCTTCAATCCCCTGTA 58
O122 FliF-F GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCTGGCTGATAACCCCTTCAATCCCCTGT 58
O123 FliF-R GTTTCTTCCTGCAGCGGCCGCTACTAGTAGTCGCATTCATCGCGCACCTCGTGGCTGA 58
O124 Oligo-pfliL-Forward-TRO TCGAATTCGCGGCCGCTTCTAGAGCAAGGGCGTGTAACAGGCAAC 45 60°C
O125 Oligo-pfliL-Reverse-TRO TCCTGCAGCGGCCGCTACTAGTAGTCATGTGTTGCGGTCTTCCTGTG 47 62°C
O126 Gene-EnvZ-F GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGGCGATTGCGCTTCTCGCCAC 53
O127 Gene-EnvZ-R-TRO GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTACCCTTCTTTTGTCGTGCCCTGCGCC 60 68°C
O128 Gene-FlgM-F GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTATTGATCGCACTTCGCCTC 53
O129 Gene-FlgM-R-TRO GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTAGTTACTCTGCAAGTCTTGCTGCGCT 59 59°C
O130 Gene-FlhC-F GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAGTGAAAAAAGCATTGTTCAGG 53
O131 Gene-FlhC-R-TRO GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAAACAGCCTGTACTCTCTGTTCATCC 60 60°C
O132 Gene-FlhD-F GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCATACCTCCGAGTTGCTGAAAC 53 Don't amplify the natural rbs of FlhD
O133 Gene-FlhD-R-TRO GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTAGGCCCTTTTCTTGCGCAGCGCTTCT 60 68°C
O134 Gene-FlhDCrbs-F GTTTCTTCGAATTCGCGGCCGCTTCTAGACCGCATAAAAATAAAGTTGGTTAT 53 Amplify the natural rbs of FlhD so that we have FlhD and FlhC with both their natural rbs
O135 xx Gene-FliA-F GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAATTCACTCTATACCGCTGAAG 53 Don't Amplify the natural rbs of FliA
O136 xx Gene-FliA-R GTTTCTTCGAATTCGCGGCCGCTTCTAGTTATTATAACTTACCCAGTTTAGTGCGTAAC 59
O137 xx Gene-FliArbs-F GTTTCTTCGAATTCGCGGCCGCTTCTAGTAATCATGCCGATAACTCATATAAC 53 Amplify the natural rbs of FliA
O138 Gene-OmpR-F GTTTCTTCGAATTCGCGGCCGCTTCTAGATGCAAGAGAACTACAAGATTCTGG 53
O139 Gene-OmpR-R GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTATGCTTTAGAGCCGTCCGGTACAAAG 60 62°C
O140 PlasTest_GFP_Rv CCCTGCAGTTAATTAATATAAACGCAGAAAGGCCAC 36 54 Amplify E0240 with a PacI and a PstI restriction site at the end
O141 PTst_GFPrbs+_F GCCTGCAGTCACACAGGAAAGTACTAGATGC 31 56 Amplify E0240 with the promoter and a PstI restriction site at the beginning
O142 PTst_GFPrbs-_F GCCTGCAGATGCGTAAAGGAGAAGAACTTTTC 32 55 Amplify E0240 without the promoter and a PstI restriction site at the beginning



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