Team:UC Berkeley

From 2008.igem.org

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|[[Team:UC_Berkeley/Team|The Team]]
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|[[Team:UC_Berkeley/ProjectOverview|Project Overview]]
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|[[Team:UC_Berkeley/LysisSystem|Sound Induced Lysis]]
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|[[Team:UC_Berkeley/GatewaySystem|Gateway Reaction]]
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|[[Team:UC_Berkeley/AssemblySystem|Assembly Reaction]]
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|[[Team:UC_Berkeley/ProteinPureSystem|Protein Purification]]
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|valign="top"| ''Our project, dubbed Lysophonix, is centered around the creation of composite parts that will cause E. coli to lyse in response to a sound stimulus. We hope to demonstrate the utility of this sound lysis device in three distinct applications: protein purification, the Gateway reaction, and composite part assembly. Current methods of protein purification abrasively disrupt the bacterial membrane, often damaging or destroying the protein of interest. We seek to show that our device can be used as a gentler alternative to release proteins from E. coli. The other two applications, the Gateway and Assembly reactions, involve passing a specific genetic sequence, often called a "part," from one plasmid to another. These processes generally require the isolation of both plasmids of interest from the E. coli in which they are amplified and the addition of somewhat costly reagents. In each case, we propose bringing together two bacterial cultures, each containing our sound lysis device and one of the necessary plasmids, that together produce all of the reagents required for the reaction. When a sound stimulus is applied, the bacteria release the plasmids and reagents into the surrounding solution and the desired reaction ensues. This promises to both simplify and reduce the costs for Gateway and Assembly reactions.''
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|align="left"|[http://spreadsheets.google.com/ccc?key=pUQEpr4ZqU9Tu4QZmdP-lKw&hl=en Our Parts]
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|align="left"|[http://spreadsheets.google.com/ccc?key=pUQEpr4ZqU9RBprtVgCq7Lg&hl=en Our Stocks]
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|align="left"|[http://spreadsheets.google.com/ccc?key=pUQEpr4ZqU9S0htjL2N6qGA&hl=en Our Oligos]
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'''In an effort to optimize the manufacture of parts, we have designed Clonebots - a collection of devices and strains that aid in the synthesis and analysis of new parts. Building engineered biological systems requires cumbersome laboratory protocols that provide a significant impediment to the advancement of our field. However, there are some unit operations that can be cost effectively automated at scale in the laboratory such as small volume liquid transfers, fluorescence measurements, and heating/cooling steps. If we can reduce all synthesis and analysis methodology to these simple operations, it will be readily possible to automate all aspects of synthetic biology research - a cost-effective, BioCAD-friendly approach to large-scale projects. The Clonebots project is an effort to solve these basic technical problems of synthetic biology with the substrate of our own medium - a live cell. We initiated the construction of several genetic devices for protein purification and standard assembly.  At the jamboree we will focus on two successfully constructed devices designed to automate sythetic biology: a genetic self-lysis device and a Gateway cloning device.'''
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<br>
----
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''Links''
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'''Navigation:'''  For a complete tour of our wiki, follow the Start arrow below.  If you want to jump straight to the devices that worked, follow the two buttons at the top of the page.  To find our notebooks or any other specific part of our wiki, follow the menu at top.
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[https://2008.igem.org/Team:UC_Berkeley_Tools UC Berkeley Comp Team]
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We thank our Berkeley iGEM Advisory Group:  Chris Anderson, Adam Arkin, John Dueber, Jay Keasling, and Susan Marqusee for their support and guidance, our Human Practices Advisors: Gaymon Bennett, Paul Rabinow, and Anthony Stavrianakis, administration support:  Kevin Costa and Kate Spohr, and the generous financial support of our sponsors:
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<a href="http://www.nsf.gov/">
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<img src="https://static.igem.org/mediawiki/2008/e/ed/Ucb_synberc.jpeg" width="130" height="130">
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<a href="http://www.invitrogen.com">
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Latest revision as of 07:16, 13 November 2008

In an effort to optimize the manufacture of parts, we have designed Clonebots - a collection of devices and strains that aid in the synthesis and analysis of new parts. Building engineered biological systems requires cumbersome laboratory protocols that provide a significant impediment to the advancement of our field. However, there are some unit operations that can be cost effectively automated at scale in the laboratory such as small volume liquid transfers, fluorescence measurements, and heating/cooling steps. If we can reduce all synthesis and analysis methodology to these simple operations, it will be readily possible to automate all aspects of synthetic biology research - a cost-effective, BioCAD-friendly approach to large-scale projects. The Clonebots project is an effort to solve these basic technical problems of synthetic biology with the substrate of our own medium - a live cell. We initiated the construction of several genetic devices for protein purification and standard assembly. At the jamboree we will focus on two successfully constructed devices designed to automate sythetic biology: a genetic self-lysis device and a Gateway cloning device.


Navigation: For a complete tour of our wiki, follow the Start arrow below. If you want to jump straight to the devices that worked, follow the two buttons at the top of the page. To find our notebooks or any other specific part of our wiki, follow the menu at top.

We thank our Berkeley iGEM Advisory Group: Chris Anderson, Adam Arkin, John Dueber, Jay Keasling, and Susan Marqusee for their support and guidance, our Human Practices Advisors: Gaymon Bennett, Paul Rabinow, and Anthony Stavrianakis, administration support: Kevin Costa and Kate Spohr, and the generous financial support of our sponsors:

Retrieved from "http://2008.igem.org/Team:UC_Berkeley"