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Team:UC Berkeley/GatewayGenomic
From 2008.igem.org
Genomic Based Gateway
Introduction
The in vivo plasmid based gateway scheme was successful and produce the desired product, but it also yielded a considerable amount of background. Closer analysis of the side products revealed that the background resulted from ccdB mutations that arose when the gene replicated and recombined in vivo. In an effort to circumvent background resulting from mutations in a negative selection gene, we decided to use positive selection. This way, we would select for the desired products rather than selecting against unwanted side products and unreacted starting materials.
