Team:UNIPV-Pavia/Notebook/Week11

From 2008.igem.org

(Difference between revisions)
Line 68: Line 68:
*We infected 9 ml LB + Amp with 30 µl of these glycerol stocks:
*We infected 9 ml LB + Amp with 30 µl of these glycerol stocks:
{|cellpadding="20px"
{|cellpadding="20px"
-
|13
+
|BBa_B0030-BBa_E0040-'''BBa_B1006'''
-
|15
+
|BBa_B0030-BBa_C0061-BBa_B1006-'''BBa_R0062'''
-
|24
+
|BBa_B0030-BBa_C0051-BBa_B0030-BBa_C0079-'''BBa_B1006'''-BBa_R0051-BBa_B0030-BBa_C0062-BBa_B1006-BBa_R0062
|-
|-
-
|25
+
|BBa_B0030-BBa_E1010-'''BBa_B1006'''
-
|34
+
|BBa_B0030-BBa_C0078-BBa_B1006-'''BBa_R0079'''
-
|35
+
|BBa_B0030-BBa_C0051-BBa_B0030-BBa_C0079-'''BBa_B1006'''-BBa_R0051-BBa_B0030-BBa_C0062-BBa_B1006
|}
|}
*(read Parts section to understand the meaning of these numbers.)
*(read Parts section to understand the meaning of these numbers.)

Revision as of 22:27, 31 July 2008


Home.jpg Home Unipv logo.jpg The Team And.jpg The Project Safety.jpg Biological Safety Dna.png Parts Submitted to the Registry
Laptop.jpg Dry Lab Pipette.jpg Wet Lab Math.gif Modeling Note.jpg Protocols Notebook.gif Activity Notebook



Notebook



Week 1 Week 2 Week 3 Week 4 Week 5 Week 6 Week 7
Week 8 Week 9 Week 10 Week 11 Week 12 Week 13 Week 14



Week 11: 07/28/08 - 08/1/08

07/28/08

  • Colony PCR for BBa_B0030-BBa_C0061-BBa_B0030-BBa_C0078-BBa_B1006-BBa_R0079 plate: 6 colonies.
Pv colonypcr 36.jpg
  • Gel results: all colonies were true positives! we decided to keep 1st colony to grow a 9 ml overnight culture.
  • Plasmid digestion for:
BBa_J23100-BBa_B0030-BBa_C0012-BBa_B1006 (E-S) BBa_R0010 (E-X)
  • Gel run/cut/gel extraction.
  • Ligation: BBa_J23100-BBa_B0030-BBa_C0012-BBa_B1006-BBa_R0010
  • We incubated ligation at 16°C overnight.
  • We infected 9 ml LB + Amp with 30 µl of these glycerol stocks:
BBa_B0030-BBa_E0040-BBa_B1006 BBa_B0030-BBa_C0061-BBa_B1006-BBa_R0062 BBa_B0030-BBa_C0051-BBa_B0030-BBa_C0079-BBa_B1006-BBa_R0051-BBa_B0030-BBa_C0062-BBa_B1006-BBa_R0062
BBa_B0030-BBa_E1010-BBa_B1006 BBa_B0030-BBa_C0078-BBa_B1006-BBa_R0079 BBa_B0030-BBa_C0051-BBa_B0030-BBa_C0079-BBa_B1006-BBa_R0051-BBa_B0030-BBa_C0062-BBa_B1006
  • (read Parts section to understand the meaning of these numbers.)
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