Team:UNIPV-Pavia/Notebook/Week17

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Revision as of 22:29, 22 September 2008

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09/8/08

We infected 9 ml of LB + Amp with 30 µl of 30(3), 31, 22, 13 and two falcon tubes for "a".

We received 3OC6HSL from Sigma! we resuspended it in ddH2O, we prepared some stocks and stored them at -20°C.

09/9/08

Ligations:
- 31-30 (="34")
- 31-22 (="35")
- 30-13 (for green fluorescence test)

@@ Line 56: / Line 56: @@
 *We prepared 0.5 l of LB + Amp for liquid cultures.
-*We infected 9 ml of LB + Amp with 30
+*We infected 9 ml of LB + Amp with 30 µl of 30(3), 31, 22, 13 and two falcon tubes for "a".
+*We received 3OC6HSL from Sigma! we resuspended it in ddH2O, we prepared some stocks and stored them at -20°C.
+'''09/9/08'''
+<br>
+*Glycerol stocks/miniprep for 30(3), 31, 22 and 13.
+*Plasmid digestion for:
+**30 (S-P)
+**31 (S-P)
+**30 (X-P)
+**22 (X-P)
+**13 (X-P)
+*Run/gel extraction.
+*Ligations:
+**31-30 (="34")
+**31-22 (="35")
+**30-13 (for green fluorescence test)
+*We induced