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Team:UNIPV-Pavia/Notebook/Week20
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'''09/29/08''' | '''09/29/08''' | ||
<br> | <br> | ||
| - | * | + | *Ligations: |
| + | **Lig.34-Lig.15 (=TR)(our RFP protein generator downstream, to check Lig.34 functionality) | ||
| + | **T5-Lig.15 (=TT)(our RFP protein generator after our GFP protein generator, to check terminator efficiency) | ||
| + | *We incubated ligations at 16°C overnight. | ||
| + | |||
| + | '''09/30/08''' | ||
| + | <br> | ||
| + | *We transformed the two overnight ligations and plated transformed bacteria. We incubated the two plates at 37°C overnight. | ||
| + | |||
| + | '''10/1/08''' | ||
| + | <br> | ||
| + | *Plates were ok! | ||
| + | |||
| + | *We performed colony PCR on the two plates. Medium and large gel could not be ran because instrumentation was buisy. We could ran two small gels (8 wells), so the maximum number of colonies was 13 (considering two markers and one blank). Whe chose to screen 6 colonies for TT and 7 colonies for TR. | ||
| + | |||
| + | *Gel results: | ||
| + | **TT - 2nd colony | ||
| + | **TR - 1st colony (gel picture not available...sorry!) | ||
Revision as of 20:25, 26 October 2008
 Home
|  The Team
|  The Project
|  Biological Safety
|  Parts Submitted to the Registry
|
|---|---|---|---|---|
 Dry Lab
|  Wet Lab
|  Modeling
|  Protocols
|  Activity Notebook
|
Notebook
| Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 |
|---|---|---|---|---|---|---|
| Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 |
| Week 15 | Week 16 | Week 17 | Week 18 | Week 19 | Week 20 | Week 21 |
Week 20: 09/29/08 - 10/3/08
09/29/08
- Ligations:
- Lig.34-Lig.15 (=TR)(our RFP protein generator downstream, to check Lig.34 functionality)
- T5-Lig.15 (=TT)(our RFP protein generator after our GFP protein generator, to check terminator efficiency)
- We incubated ligations at 16°C overnight.
09/30/08
- We transformed the two overnight ligations and plated transformed bacteria. We incubated the two plates at 37°C overnight.
10/1/08
- Plates were ok!
- We performed colony PCR on the two plates. Medium and large gel could not be ran because instrumentation was buisy. We could ran two small gels (8 wells), so the maximum number of colonies was 13 (considering two markers and one blank). Whe chose to screen 6 colonies for TT and 7 colonies for TR.
- Gel results:
- TT - 2nd colony
- TR - 1st colony (gel picture not available...sorry!)
