Team:UNIPV-Pavia/Notebook/Week8

From 2008.igem.org

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**No true positives for BBa_J23100-'''BBa_B0030'''-BBa_C0012
**No true positives for BBa_J23100-'''BBa_B0030'''-BBa_C0012
**No true positives for BBa_J23100-'''BBa_B0030'''-BBa_I15010
**No true positives for BBa_J23100-'''BBa_B0030'''-BBa_I15010
 +
**Non pure true positives for BBa_B0030-BBa_E0040-'''BBa_B1006'''
**Pure true positives for BBa_B0030-BBa_C0051-'''BBa_B1006'''
**Pure true positives for BBa_B0030-BBa_C0051-'''BBa_B1006'''
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**
+
**Non pure true positives for BBa_B0030-BBa_E1010-'''BBa_B1006'''
 +
 
 +
*We chose to keep the first colony for all the 3 working ligation plates.
 +
 
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*NOTE: BBa_B0030-BBa_E0040-'''BBa_B1006''' and BBa_B0030-BBa_E1010-'''BBa_B1006''' are final parts; we decided to sequence these 2 parts even if gel showed a weak contamination: sequencing results will tell us if there are false positive plasmids in those colonies or if the contamination was only a PCR contamination.
 +
 
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*We infected 9 ml LB + suitable antibiotic with 30

Revision as of 10:24, 20 July 2008


Home.jpg Home Unipv logo.jpg The Team And.jpg The Project Safety.jpg Biological Safety Dna.png Parts Submitted to the Registry
Laptop.jpg Dry Lab Pipette.jpg Wet Lab Math.gif Modeling Note.jpg Protocols Notebook.gif Activity Notebook



Notebook



Week 1 Week 2 Week 3 Week 4 Week 5 Week 6 Week 7
Week 8 Week 9 Week 10 Week 11 Week 12 Week 13 Week 14



Week 8: 07/7/08 - 07/12/08

07/7/08

  • Colony PCR (5 colonies for each plate) for:
    • BBa_J23100-BBa_B0030-BBa_C0012 (for the second time, we hoped to find true positive colonies)
    • BBa_J23100-BBa_B0030-BBa_I15010 (for the second time, we hoped to find true positive colonies)
    • BBa_B0030-BBa_E0040-BBa_B1006
    • BBa_B0030-BBa_C0051-BBa_B1006
    • BBa_B0030-BBa_E1010-BBa_B1006
Colony PCR: Marker (1), empty (2) BBa_J23100-BBa_B0030-BBa_C0012, BBa_J23100-BBa_B0030-BBa_I15010, BBa_B0030-BBa_E0040-BBa_B1006, BBa_B0030-BBa_C0051-BBa_B1006, BBa_B0030-BBa_E1010-BBa_B1006, blank
  • Gel results:
    • No true positives for BBa_J23100-BBa_B0030-BBa_C0012
    • No true positives for BBa_J23100-BBa_B0030-BBa_I15010
    • Non pure true positives for BBa_B0030-BBa_E0040-BBa_B1006
    • Pure true positives for BBa_B0030-BBa_C0051-BBa_B1006
    • Non pure true positives for BBa_B0030-BBa_E1010-BBa_B1006
  • We chose to keep the first colony for all the 3 working ligation plates.
  • NOTE: BBa_B0030-BBa_E0040-BBa_B1006 and BBa_B0030-BBa_E1010-BBa_B1006 are final parts; we decided to sequence these 2 parts even if gel showed a weak contamination: sequencing results will tell us if there are false positive plasmids in those colonies or if the contamination was only a PCR contamination.
  • We infected 9 ml LB + suitable antibiotic with 30