Team:UNIPV-Pavia/Notebook/Week9

From 2008.igem.org

(Difference between revisions)
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'''07/14/08'''
'''07/14/08'''
<br>
<br>
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*We transformed (1)
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*We transformed 1 µl of the 6 ligations stored at -20°C. We plated transformed bacteria and incubated plates at 37°C overnight.
 +
 
 +
*Plasmid digestion for:
 +
{|cellpadding="20px"
 +
|BBa_R0079 (E-X)
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|BBa_B0030-BBa_C0078-'''BBa_B1006''' (E-S)
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|}
 +
 
 +
*Gel run/cut/gel extraction for the 2 parts.
 +
 
 +
*Ligation: BBa_B0030-BBa_C0078-BBa_B1006-'''BBa_R0079'''.
 +
 
 +
*We incubated ligation at 16°C overnight.
 +
 
 +
<br><br>
 +
'''07/14/08'''
 +
<br>
 +
*We transformed 1 µl of BBa_B0030-BBa_C0078-BBa_B1006-'''BBa_R0079''' ligation and plated transformed bacteria. We incubated the plate at 37°C overnight.
 +
 
 +
*All the 6 overnight plates showed colonies. We performed colony PCR for:
 +
**

Revision as of 13:56, 20 July 2008


Home.jpg Home Unipv logo.jpg The Team And.jpg The Project Safety.jpg Biological Safety Dna.png Parts Submitted to the Registry
Laptop.jpg Dry Lab Pipette.jpg Wet Lab Math.gif Modeling Note.jpg Protocols Notebook.gif Activity Notebook



Notebook



Week 1 Week 2 Week 3 Week 4 Week 5 Week 6 Week 7
Week 8 Week 9 Week 10 Week 11 Week 12 Week 13 Week 14



Week 8: 07/14/08 - 07/17/08

07/14/08

  • We transformed 1 µl of the 6 ligations stored at -20°C. We plated transformed bacteria and incubated plates at 37°C overnight.
  • Plasmid digestion for:
BBa_R0079 (E-X) BBa_B0030-BBa_C0078-BBa_B1006 (E-S)
  • Gel run/cut/gel extraction for the 2 parts.
  • Ligation: BBa_B0030-BBa_C0078-BBa_B1006-BBa_R0079.
  • We incubated ligation at 16°C overnight.



07/14/08

  • We transformed 1 µl of BBa_B0030-BBa_C0078-BBa_B1006-BBa_R0079 ligation and plated transformed bacteria. We incubated the plate at 37°C overnight.
  • All the 6 overnight plates showed colonies. We performed colony PCR for:
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