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Team:UNIPV-Pavia/Notebook/Week9
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Notebook
| Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 |
|---|---|---|---|---|---|---|
| Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 |
Week 8: 07/14/08 - 07/17/08
07/14/08
- We transformed 1 µl of the 6 ligations stored at -20°C. We plated transformed bacteria and incubated plates at 37°C overnight.
- Plasmid digestion for:
| BBa_R0079 (E-X) | BBa_B0030-BBa_C0078-BBa_B1006 (E-S) |
- Gel run/cut/gel extraction for the 2 parts.
- Ligation: BBa_B0030-BBa_C0078-BBa_B1006-BBa_R0079.
- We incubated ligation at 16°C overnight.
07/15/08
- We transformed 1 µl of BBa_B0030-BBa_C0078-BBa_B1006-BBa_R0079 ligation and plated transformed bacteria. We incubated the plate at 37°C overnight.
- All the 6 overnight plates showed colonies. We performed colony PCR for (we decided to pick up more colonies for the ligations with long inserts):
- BBa_J23100-BBa_B0030 (S-P) -BBa_C0012 (standard ligation) - 10 colonies
- pSB1AK3-BBa_J23100-BBa_B0030-BBa_C0012 (double ligation) - 7 colonies
- BBa_J23100-BBa_B0030-BBa_C0040-BBa_B1006-BBa_R0040 - 3 colonies
- BBa_R0051-BBa_B0030-BBa_C0062-BBa_B1006-BBa_R0062 - 3 colonies
- BBa_B0030-BBa_C0051-BBa_B0030-BBa_C0079-BBa_B1006 - 10 colonies
- BBa_B0030-BBa_I15009-BBa_B1006 - 3 colonies
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