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Team:Valencia/Notebook/Protocols
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| + | == Glycerol Stock == | ||
| + | 2 ml Eppendorf containing: <br> | ||
| + | Sterile Glycerol (stock 50 %) 1ml <br> | ||
| + | Half culture plate <br> | ||
| + | |||
== SD Medium == | == SD Medium == | ||
Revision as of 09:11, 7 August 2008
Glycerol Stock
2 ml Eppendorf containing:
Sterile Glycerol (stock 50 %) 1ml
Half culture plate
SD Medium
Yeast Nitrogen Base 0.67%
Glucose 2%
Agar 2%
Casamino acids 0.1%
Tryptophan 20 mg/l
Adenine 40 mg/l
SP liquid medium
Yeast Nitrogen Base 0.67%
Potassium phosohate 0.1%
Ammonium sulfate 0.12%
Glucose 0.05%
Lactic acid 2%
Casamino acids 0.1%
Tryptophan 20 mg/l
Adenine 40 mg/l
YPKAc Medium (Meri's medium)
Yeast extract 1%
Peptone 2%
Potassium acetate 2%
Tryptophan 20 mg/l
Adenine 40 mg/l
