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Team:Waterloo/Notebook/Oligos
From 2008.igem.org
(Difference between revisions)
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|T7gene6 ORF (rev) || T7gs-R || CTGCAGCGGCCGCTACTAGTATTATTACGGTCTCCACAGGTAAATCTC || 48 | |T7gene6 ORF (rev) || T7gs-R || CTGCAGCGGCCGCTACTAGTATTATTACGGTCTCCACAGGTAAATCTC || 48 | ||
|- | |- | ||
| - | | || T7g6-RXO-RBS || CTAGTATTTCTCCTCTTTCTCTAGTATTATTACGGTCTCCACAGGTAAATCTC || 53 | + | |T7gene6 ORF rev; for joining to PmeI with cross over PCR || T7g6-RXO-RBS || CTAGTATTTCTCCTCTTTCTCTAGTATTATTACGGTCTCCACAGGTAAATCTC || 53 |
|- | |- | ||
| - | | || RBSpmeI-F || GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGACAACAAACTCCCCCTCAG || 61 | + | |PmeI ORF (fwd; for adding prefix-RBS) || RBSpmeI-F || GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGACAACAAACTCCCCCTCAG || 61 |
|- | |- | ||
| - | | | + | |PmeI ORF (fwd; for adding prefix-RBS and joining to T7gene6 by crossover PCR) || RBSpmeI-FXO || TACTAGAGAAAGAGGAGAAATACTAGATGACAACAAACTCCCCCTCAG || 48 |
|- | |- | ||
| - | | || pmeI-R || CTGCAGCGGCCGCTACTAGTATTATTAAACCGTACTTCGCCCCCG || 45 | + | |PmeI ORF (rev; TAATAA stop codon-suffix) || pmeI-R || CTGCAGCGGCCGCTACTAGTATTATTAAACCGTACTTCGCCCCCG || 45 |
|- | |- | ||
| - | | | + | |Primer for part R0011 (Plac-PL hybrid promoter; fwd) || R0011-F || GAATTCCGGCCGCTTCTAGAGAATTGTGAGCGGATAAC || 38 |
|- | |- | ||
| - | | | + | |Primer for part R0011 (Plac-PL hybrid promoter; rev for joining to ORFs with RBS by crossover PCR)|| R0011RBS-RXO || CTAGTATTTCTCCTCTTTCTCTAGTATGTGCTCAGTATCTTGTTATC || 47 |
|- | |- | ||
| - | | | + | |CI ORF (fwd; prefix-RBS) || RBSC0051-F || GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGAGCACAAAAAAGAAACC || 59 |
|- | |- | ||
| - | | | + | |CI ORF (fwd; prefix-RBS for joining to R0011 promoter by cross over PCR) || RBSC0051-FXO || TACTAGAGAAAGAGGAGAAATACTAGATGAGCACAAAAAAGAAACC || 46 |
|- | |- | ||
| - | | || C0051-R || CTGCAGCGGCCGCTACTAGTATTATTAAGCTACTAAAGCG || 40 | + | |CI ORF (rev; suffix adapter) || C0051-R || CTGCAGCGGCCGCTACTAGTATTATTAAGCTACTAAAGCG || 40 |
|- | |- | ||
| - | | || C0051-RXO || GATGCCTGGCTCTAGTATTATTAAGCTACTAAAGCG || 36 | + | |CI ORF (rev; for joining to transcriptional terminator (b0015) by cross over PCR) || C0051-RXO || GATGCCTGGCTCTAGTATTATTAAGCTACTAAAGCG || 36 |
|- | |- | ||
| - | | || B0015-FXO || TAATAATACTAGAGCCAGGCATCAAATAAAACG || 33 | + | |Transcriptional terminator (B0015; fwd; for joining to ORFs by cross over PCR) || B0015-FXO || TAATAATACTAGAGCCAGGCATCAAATAAAACG || 33 |
|- | |- | ||
| - | | || B0015-R || CTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCC || 39 | + | |Transcriptional terminator (B0015; rev) || B0015-R || CTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCC || 39 |
|- | |- | ||
| - | | || RFP-RBS-F || GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACG || 58 | + | |RFP ORF (fwd; prefix-RBS) || RFP-RBS-F || GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACG || 58 |
|- | |- | ||
| - | | | + | |RFP ORF (fwd; prefix-RBS for joining to transcriptional terminator by cross over PCR ) || RFP-RXO || GATGCCTGGCTCTAGTATTATTAAGCACCGGTGG || 34 |
|- | |- | ||
| - | | | + | |Plac (r0011; fwd; for adding lac promoter and RBS to ORFs by PCR) || Plac-RBS-F || GAATTCGCGGCCGCTTCTAGAGAATTGTGAGCGGATAACAATTGACATTGTGAGCGGATAACAAGATACTGAGCAC ATACTAGAGAAAGAGGAGAAATACTAG || 103 |
|} | |} | ||
| + | <br> | ||
| + | * ' denotes that the construct contains the ribosome binding site (RBS) and the coding region for that gene. | ||
| + | * ' ' denotes that the construct contains the ribosome binding site, coding region for that gene, and a transcriptional terminator. | ||
Revision as of 03:14, 30 October 2008
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| Description | Oligo Name | Sequence | Length |
|---|---|---|---|
| BioBrick sequencing/colPCR primer (fwd) | iGEMvf2 | TGCCACCTGACGTCTAAGAA | 20 |
| BioBrick sequencing/colPCR primer (rev) | iGEMvr | ATTACCGCCTTTGAGTGAGC | 20 |
| Primer for Lac promoter (fwd) | PLacF | TTATGCTTCCGGCTCGTATG | 20 |
| Primer for {BioBrick Prefix}-{B0034} (rev; for use with iGEMvf2) | iGEMpre+rbs | TTTCTCCTCTTTCTCTAGATGCGG | 24 |
| Primer for {BioBrick Prefix}-{B0034} (fwd; for use with iGEMvr) | PrefixRBS-F | GGCCGCTTCTAGAGAAAGAGG | 21 |
| Gene 5 ORF from T7 phage (fwd; Prefix adapter) | T7gene5-F | CAATGGAATTCGCGGCCGCTTCTAGATGATCGTTTCTGACATCGAA | 46 |
| Gene 5 ORF from T7 phage (rev; Suffix adapter) | T7gene5-R | CAATTTCTGCAGCGGCCGCTACTAGTATCAGTGGCAAATCGCCCAATTAGG | 51 |
| Gene 6 ORF from T7 phage (fwd; Prefix adapter) | T7gene6-F | CAATGGAATTCGCGGCCGCTTCTAGATGGCACTTCTTGACCTTAAA | 46 |
| Gene 6 ORF from T7 phage (rev; Suffix adapter) | T7gene6-R | CAATTTCTGCAGCGGCCGCTACTAGTACTACGGTCTCCACAGG | 43 |
| PmeIR ORF (fwd) | PmeIR-F | ATCCGGAATTCGCGGCGCTTTCTAGATGACAACAAACTCCCCCTCAGACG | 50 |
| PmeIR ORF (rev) | PmelR-R | CGCAAAACTGCAGCGGCCGCTACTAGTATCAAACCGTACTTCGCCCCCGTTG | 52 |
| pRecA oligo (sense strand; produces BioBrick-compatible EcoRI/SpeI overhangs when annealed to recAR) | recAF | AATTCGCGGCCGCTTCTAGAGTACAAAACACTTGATACTGTATATATATACAGTATAATTGCTTCAACATA | 71 |
| pRecA oligo (antisense strand; produces BioBrick-compatible EcoRI/SpeI overhangs when annealed to recAR) | recAR | CTAGTATGTTGAAGCAATTATACTGTATATATATACAGTATCAAGTGTTTTGTACTCTAGAAGCGGCCGCG | 71 |
| {B0034}-{T7gene6 ORF} (fwd; for adding {Prefix-RBS} onto the gene 6 ORF by PCR) | RBST7g6-F | GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCACTTCTTGACCTTAAAC | 61 |
| T7gene6 ORF (rev) | T7gs-R | CTGCAGCGGCCGCTACTAGTATTATTACGGTCTCCACAGGTAAATCTC | 48 |
| T7gene6 ORF rev; for joining to PmeI with cross over PCR | T7g6-RXO-RBS | CTAGTATTTCTCCTCTTTCTCTAGTATTATTACGGTCTCCACAGGTAAATCTC | 53 |
| PmeI ORF (fwd; for adding prefix-RBS) | RBSpmeI-F | GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGACAACAAACTCCCCCTCAG | 61 |
| PmeI ORF (fwd; for adding prefix-RBS and joining to T7gene6 by crossover PCR) | RBSpmeI-FXO | TACTAGAGAAAGAGGAGAAATACTAGATGACAACAAACTCCCCCTCAG | 48 |
| PmeI ORF (rev; TAATAA stop codon-suffix) | pmeI-R | CTGCAGCGGCCGCTACTAGTATTATTAAACCGTACTTCGCCCCCG | 45 |
| Primer for part R0011 (Plac-PL hybrid promoter; fwd) | R0011-F | GAATTCCGGCCGCTTCTAGAGAATTGTGAGCGGATAAC | 38 |
| Primer for part R0011 (Plac-PL hybrid promoter; rev for joining to ORFs with RBS by crossover PCR) | R0011RBS-RXO | CTAGTATTTCTCCTCTTTCTCTAGTATGTGCTCAGTATCTTGTTATC | 47 |
| CI ORF (fwd; prefix-RBS) | RBSC0051-F | GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGAGCACAAAAAAGAAACC | 59 |
| CI ORF (fwd; prefix-RBS for joining to R0011 promoter by cross over PCR) | RBSC0051-FXO | TACTAGAGAAAGAGGAGAAATACTAGATGAGCACAAAAAAGAAACC | 46 |
| CI ORF (rev; suffix adapter) | C0051-R | CTGCAGCGGCCGCTACTAGTATTATTAAGCTACTAAAGCG | 40 |
| CI ORF (rev; for joining to transcriptional terminator (b0015) by cross over PCR) | C0051-RXO | GATGCCTGGCTCTAGTATTATTAAGCTACTAAAGCG | 36 |
| Transcriptional terminator (B0015; fwd; for joining to ORFs by cross over PCR) | B0015-FXO | TAATAATACTAGAGCCAGGCATCAAATAAAACG | 33 |
| Transcriptional terminator (B0015; rev) | B0015-R | CTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCC | 39 |
| RFP ORF (fwd; prefix-RBS) | RFP-RBS-F | GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACG | 58 |
| RFP ORF (fwd; prefix-RBS for joining to transcriptional terminator by cross over PCR ) | RFP-RXO | GATGCCTGGCTCTAGTATTATTAAGCACCGGTGG | 34 |
| Plac (r0011; fwd; for adding lac promoter and RBS to ORFs by PCR) | Plac-RBS-F | GAATTCGCGGCCGCTTCTAGAGAATTGTGAGCGGATAACAATTGACATTGTGAGCGGATAACAAGATACTGAGCAC ATACTAGAGAAAGAGGAGAAATACTAG | 103 |
- ' denotes that the construct contains the ribosome binding site (RBS) and the coding region for that gene.
- ' ' denotes that the construct contains the ribosome binding site, coding region for that gene, and a transcriptional terminator.
