Team:Waterloo/Notebook/Oligos

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(Difference between revisions)

Revision as of 17:39, 29 October 2008

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Description	Oligo Name	Sequence	Length
BioBrick sequencing/colPCR primer (fwd)	iGEMvf2	TGCCACCTGACGTCTAAGAA	20
BioBrick sequencing/colPCR primer (rev)	iGEMvr	ATTACCGCCTTTGAGTGAGC	20
Primer for Lac promoter (fwd)	PLacF	TTATGCTTCCGGCTCGTATG	20
Primer for {BioBrick Prefix}-{B0034} (rev; for use with iGEMvf2)	iGEMpre+rbs	TTTCTCCTCTTTCTCTAGATGCGG	24
Gene 5 ORF from T7 phage (fwd)	T7gene5-F	CAATGGAATTCGCGGCCGCTTCTAGATGATCGTTTCTGACATCGAA	46
Gene 5 ORF from T7 phage (rev)	T7gene5-R	CAATTTCTGCAGCGGCCGCTACTAGTATCAGTGGCAAATCGCCCAATTAGG	51
Gene 6 ORF from T7 phage (fwd)	T7gene6-F	CAATGGAATTCGCGGCCGCTTCTAGATGGCACTTCTTGACCTTAAA	46
Gene 6 ORF from T7 phage (rev)	T7gene6-R	CAATTTCTGCAGCGGCCGCTACTAGTACTACGGTCTCCACAGG	43
	PrefixRBS-F	GGCCGCTTCTAGAGAAAGAGG	21
	PmeIR-F	ATCCGGAATTCGCGGCGCTTTCTAGATGACAACAAACTCCCCCTCAGACG	50
	PmelR-R	CGCAAAACTGCAGCGGCCGCTACTAGTATCAAACCGTACTTCGCCCCCGTTG	52
	phbA-F	GAATTCGCGGCCGCTTCTAGATGAGCAATCCCTCGATCG	39
	phbA-R	CTGCAGCGGCCGCTACTAGTATTACAGGCGTTCCACGCACATGG	44
	phbB-F	GAATTCGCGGCCGCTTCTAGATGAGCAGGGTAGCACTGG	39
	phbB-R	CTGCAGCGGCCGCTACTAGTATCAGGCGAAGTACTGGCCGC	41
	phbC-F	GAATTCGCGGCCGCTTCTAGATGACAGCAGAGAAGGCTGAGG	42
	phbC-R	CTGCAGCGGCCGCTACTAGTATCAGGCGCGCACGCGCACGTAGG	44
	recAF	AATTCGCGGCCGCTTCTAGAGTACAAAACACTTGATACTGTATATATATACAGTATAATTGCTTCAACATA	71
	recAR	CTAGTATGTTGAAGCAATTATACTGTATATATATACAGTATCAAGTGTTTTGTACTCTAGAAGCGGCCGCG	71
	RBST7g6-F	GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCACTTCTTGACCTTAAAC	61
	T7gs-R	CTGCAGCGGCCGCTACTAGTATTATTACGGTCTCCACAGGTAAATCTC	48
	T7g6-RXO-RBS	CTAGTATTTCTCCTCTTTCTCTAGTATTATTACGGTCTCCACAGGTAAATCTC	53
	RBSpmeI-F	GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGACAACAAACTCCCCCTCAG	61
	RBSpmeI-FXO	TACTAGAGAAAGAGGAGAAATACTAGATGACAACAAACTCCCCCTCAG	48
	pmeI-R	CTGCAGCGGCCGCTACTAGTATTATTAAACCGTACTTCGCCCCCG	45
	R0011-F	GAATTCCGGCCGCTTCTAGAGAATTGTGAGCGGATAAC	38
	R0011RBS-RXO	CTAGTATTTCTCCTCTTTCTCTAGTATGTGCTCAGTATCTTGTTATC	47
	RBSC0051-F	GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGAGCACAAAAAAGAAACC	59
	RBSC0051-FXO	TACTAGAGAAAGAGGAGAAATACTAGATGAGCACAAAAAAGAAACC	46
	C0051-R	CTGCAGCGGCCGCTACTAGTATTATTAAGCTACTAAAGCG	40
	C0051-RXO	GATGCCTGGCTCTAGTATTATTAAGCTACTAAAGCG	36
	B0015-FXO	TAATAATACTAGAGCCAGGCATCAAATAAAACG	33
	B0015-R	CTGCAGCGGCCGCTACTAGTATATAAACGCAGAAAGGCC	39
	RFP-RBS-F	GAATTCCGGCCGCTTCTAGAGAAAGAGGAGAAATACTAGATGGCTTCCTCCGAAGACG	58
	RFP-RXO	GATGCCTGGCTCTAGTATTATTAAGCACCGGTGG	34
	Plac-RBS-F	GAATTCGCGGCCGCTTCTAGAGAATTGTGAGCGGATAACAATTGACATTGTGAGCGGATAACAAGATACTGAGCACATACTAGAGAAAGAGGAGAAATACTAG	103

@@ Line 15: / Line 15: @@
 !width="500"|Length
 |-
-|  || iGEMvr || ATTACCGCCTTTGAGTGAGC || 20
+|BioBrick sequencing/colPCR primer (fwd)  || iGEMvf2 || TGCCACCTGACGTCTAAGAA || 20
 |-
-|  || PLacF || TTATGCTTCCGGCTCGTATG || 20
+|BioBrick sequencing/colPCR primer (rev)  || iGEMvr || ATTACCGCCTTTGAGTGAGC || 20
 |-
-|  || iGEMpre+rbs || TTTCTCCTCTTTCTCTAGATGCGG || 24
+|Primer for Lac promoter (fwd)  || PLacF || TTATGCTTCCGGCTCGTATG || 20
 |-
-|  || iGEMvf2 || TGCCACCTGACGTCTAAGAA || 20
+|Primer for {BioBrick Prefix}-{B0034} (rev; for use with iGEMvf2)|| iGEMpre+rbs || TTTCTCCTCTTTCTCTAGATGCGG || 24
 |-
-|  || T7gene5-F || CAATGGAATTCGCGGCCGCTTCTAGATGATCGTTTCTGACATCGAA || 46
+|Gene 5 ORF from T7 phage (fwd)  || T7gene5-F || CAATGGAATTCGCGGCCGCTTCTAGATGATCGTTTCTGACATCGAA || 46
 |-
-|  || T7gene6-F || CAATGGAATTCGCGGCCGCTTCTAGATGGCACTTCTTGACCTTAAA || 46
+|Gene 5 ORF from T7 phage (rev)  || T7gene5-R || CAATTTCTGCAGCGGCCGCTACTAGTATCAGTGGCAAATCGCCCAATTAGG || 51
 |-
-|  || T7gene6-R || CAATTTCTGCAGCGGCCGCTACTAGTACTACGGTCTCCACAGG || 43
+|Gene 6 ORF from T7 phage (fwd)  || T7gene6-F || CAATGGAATTCGCGGCCGCTTCTAGATGGCACTTCTTGACCTTAAA || 46
 |-
-|  || PrefixRBS-F || GGCCGCTTCTAGAGAAAGAGG || 21
+|Gene 6 ORF from T7 phage (rev)  || T7gene6-R || CAATTTCTGCAGCGGCCGCTACTAGTACTACGGTCTCCACAGG || 43
 |-
-|  || T7gene5-R || CAATTTCTGCAGCGGCCGCTACTAGTATCAGTGGCAAATCGCCCAATTAGG || 51
+|  || PrefixRBS-F || GGCCGCTTCTAGAGAAAGAGG || 21
 |-
 |  || PmeIR-F || ATCCGGAATTCGCGGCGCTTTCTAGATGACAACAAACTCCCCCTCAGACG || 50