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Team:Waterloo/Project
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The primary application of this would be an in situ compound production and delivery system for agricultural and/or therapeutic uses. | The primary application of this would be an in situ compound production and delivery system for agricultural and/or therapeutic uses. | ||
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== Project Details== | == Project Details== | ||
Revision as of 02:29, 11 July 2008
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Overall project
Our goal is to engineer a genome-free, cell-based expression system capable of producing a desired protein in response to environmental signals. The genome will be degraded by the combined activity of a restriction endonuclease (to fragment the genome) and an exonuclease (to hasten degradation of the genome). The gene for the protein of interest will be located on a plasmid which will lack recognition sites for the endonuclease, enabling it to remain intact after genome degradation. Expression of plasmid genes is expected to continue for a period of time until the "cell" expires.
The primary application of this would be an in situ compound production and delivery system for agricultural and/or therapeutic uses.
