Alberta NINT/25 June 2008

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[[Alberta_NINT/24_June_2008 | < Previous entry]]
[[Alberta_NINT/24_June_2008 | < Previous entry]]
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lab work (SD):
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            Isolated DNA (K102008) from 24/06/08 overnights using QIAprep spin miniprep protocol.
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            Set up K102008 for sequencing.
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            Digested K102002 and K102008 with EcoRI + PstI, ran on 2% agarose gel at 110V for 50 min.
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            Excised large fragments from the gel.
lab work (JD):
lab work (JD):
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               Decided to try AGAIN to recreate K102007 but using older versions of K102005/B+N that seemed to be cut better when looking at gels and ligating them to the newly           annealed TA0/B+N (a combination I still haven't tried)
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               Decided to try AGAIN to recreate K102007 but using older versions of K102005/B+N that seemed to be
 +
              cut better when looking at gels and ligating them to the newly annealed TA0/B+N (a combination I
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              still haven't tried)
               Created K102007(1): K102005/B+N from June 18, and TA0/B+N newly annealed
               Created K102007(1): K102005/B+N from June 18, and TA0/B+N newly annealed
               Created K102007(2): K102005/B+N from June 12 and TA0/B+N newly annealed
               Created K102007(2): K102005/B+N from June 12 and TA0/B+N newly annealed

Revision as of 17:05, 27 June 2008

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lab work (SD): 

            Isolated DNA (K102008) from 24/06/08 overnights using QIAprep spin miniprep protocol.
            Set up K102008 for sequencing.
            Digested K102002 and K102008 with EcoRI + PstI, ran on 2% agarose gel at 110V for 50 min.
            Excised large fragments from the gel.

lab work (JD): 

             Decided to try AGAIN to recreate K102007 but using older versions of K102005/B+N that seemed to be
             cut better when looking at gels and ligating them to the newly annealed TA0/B+N (a combination I
             still haven't tried)
             Created K102007(1): K102005/B+N from June 18, and TA0/B+N newly annealed
             Created K102007(2): K102005/B+N from June 12 and TA0/B+N newly annealed
             Created K102006: K102003/B+N and TA0/B+N newly annealed
             Transformed XL1-B cells with the ligations and negative controls
             Plated cells

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