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Alberta NINT/28 May 2008
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| + | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
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| + | [[Alberta_NINT/27_May_2008 | < Previous entry]] | [[Alberta_NINT/29_May_2008 | Next entry >]] | ||
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lab work (SD): | lab work (SD): | ||
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Digested R0011 with SpeI+ PstI | Digested R0011 with SpeI+ PstI | ||
Ran gel electrophoresis and cut out desired band. | Ran gel electrophoresis and cut out desired band. | ||
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| + | lab work (WM): | ||
| + | Ligated I0500/(SpeI + PstI) with TA0In/(XbaI + PstI) | ||
| + | Transformed 2 ul into 100 ul XL1-B. | ||
| + | Plated 150 ul on LB + kan35 | ||
Latest revision as of 21:38, 11 October 2008
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lab work (SD):
Transformed XL1-B cells with the plasmids created 27/05/08.
K102002 had 170 colonies. K102004 had 629 colonies. K102000 negative control had 5 colonies.
lab work (JD):
Carried out mini-preps on O/Ns
Digested R0011 with SpeI+ PstI
Ran gel electrophoresis and cut out desired band.
lab work (WM):
Ligated I0500/(SpeI + PstI) with TA0In/(XbaI + PstI)
Transformed 2 ul into 100 ul XL1-B.
Plated 150 ul on LB + kan35
