Edinburgh/17 September 2008
From 2008.igem.org
(Difference between revisions)
(New page: <div id="header">{{Template:Team:Edinburgh/Templates/Header}}</div> '''< Previous Entry | Notebook Index | [[Edinburgh/18_Septe...) |
|||
Line 4: | Line 4: | ||
== Week 14 == | == Week 14 == | ||
- | === | + | === Wednesday 17 September 08 === |
* Sent M211-212 (pSB1A2-glgC triple mut), M215 (pSB1A2-bglX) for sequencing. (AM) | * Sent M211-212 (pSB1A2-glgC triple mut), M215 (pSB1A2-bglX) for sequencing. (AM) |
Revision as of 18:16, 6 October 2008
< Previous Entry | Notebook Index | Next Entry >
Week 14
Wednesday 17 September 08
- Sent M211-212 (pSB1A2-glgC triple mut), M215 (pSB1A2-bglX) for sequencing. (AM)
- P97: Third attempt at MABEL PCR for cex using M150 as template with 10µl 50% glycerol, denaturing 1 min, annealing 58°C, extension 95 sec. (AM)
- Re-run M213, M217-M221 (dxs+CrtEBI) on Gel 79, results looked promising and M213 and M218 will be sent for sequencing tomorrow. (YAN)
- Decided to give up on cenA and use CHU1727 endoglucanase from Cytophaga instead. CKL (CF's PhD student) is working on this. (AM)