Team:KULeuven/Project/Output

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===Components===
===Components===
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We added C-terminal a [https://2008.igem.org/Team:KULeuven/Literature#LVA_tag LVA tag] for rapid degradation of the protein, so that we can follow the output signal quickly in time. The OmpF promoter (http://partsregistry.org/Part:BBa_R0084 '''BBa_R0084''']) behaves as described in the [https://2008.igem.org/Team:KULeuven/Project/Input input] section. For fine-tuning reasons, the RBS was chosen [http://partsregistry.org/Part:BBa_B0032 '''BBa_B0032'''] with a relative efficiency of 0,3.
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We added C-terminal a [https://2008.igem.org/Team:KULeuven/Literature#LVA_tag LVA tag] for rapid degradation of the protein, so that we can follow the output signal quickly in time. The OmpF promoter ([http://partsregistry.org/Part:BBa_R0084 '''BBa_R0084''']) behaves as described in the [https://2008.igem.org/Team:KULeuven/Project/Input input] section. For fine-tuning reasons, the RBS was chosen [http://partsregistry.org/Part:BBa_B0032 '''BBa_B0032'''] with a relative efficiency of 0,3.
===Action===
===Action===

Revision as of 18:46, 30 July 2008

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Pictogram output.png

Contents

Output

We have been searching for an easy accesible output system, that could us provide in a quick and easy way qualitative and quantitative information. The choice for GFP was quickly made.

BioBricks

Output BioBrick.jpg

Components

We added C-terminal a LVA tag for rapid degradation of the protein, so that we can follow the output signal quickly in time. The OmpF promoter ([http://partsregistry.org/Part:BBa_R0084 BBa_R0084]) behaves as described in the input section. For fine-tuning reasons, the RBS was chosen [http://partsregistry.org/Part:BBa_B0032 BBa_B0032] with a relative efficiency of 0,3.

Action

This is a very easy system. As the input results in a low concentration of OmpR, there is GFP production, proportional to the input signal.

PI remarks