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Alberta NINT/10 July 2008
From 2008.igem.org
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lab work (SD):
Inoculated 96-well plate with LB + amp50, LB + amp50 + kan30, and LB + tet10 and four colonies from
each plate of the transformants from 07/07/08.
K102022 in LB + amp50
K102032 in LB + amp50
K102032 + K102011 in LB + amp50 + kan30
K102032 + K102034 in LB + amp50 + kan30
K102032 + K102035 in LB + amp50 + kan30
K102032 + K102036 in LB + amp50 + kan30
XL1-B in LB + tet10
lab work (JD):
Carried out Day1 procedure of Galactosidase Activity Assay
Mini-prep of pSB4A5.1 and pSB4A5.2 where pSB4A5.1=lab DB3.1 cells, pSB4A5.2=commercial DB3.1 cells
Restriction digest, pSB4A5/EcoR1 and Pst1
Ran gel electrophoresis on 2% gel at 100V
Gel purification of pieces
