26 October 2008
Biobricking work
- Made new culture of the PVJ4 transformation so that a miniprep can be done.
- New culture of gene S105 part made.
Began transforming promoters from the registry in order to ligate them to our cassette and create composite parts:
- pBAD prmoter
- IPTG inducible promoter
- Mercury inducible promoter
- Pu inducible promoter (xylene induced)
- T7 promoter, with activity modified by change in temperature
Also re-transformed ligations of the pVJ4 cassette and the S105 cassette (both transformations unsuccessful in the past)
- Will run a gel of the S105 gene restriction digest with EcoRI and SpeI for verification of part. The gel showed a faint band at the length we hoped for but there was no other band verifying the vector. We should have seen two bands but we only saw one.
Conductivity Tests
- PVJ4 Colony 2 and 4 plus Controls. 10ml cultures. 420 ul of 50% Arabinose solution will be added. Conductance measurements will be taken before and after lysis has occurred.
- Throughout the test, Optical Density measurements will be taken.
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