Team:Chiba/Calendar-Home/6 September 2008
From 2008.igem.org
5 September 2008 <|> 7 September 2008
Contents |
Meeting with UmeG
the ppt (in Japanese) for our weekly meeting
Laboratory work
Team:Input
no work
Team:Communication
DNA Template 1 dNTP mix 5 Foward Primer 5 Reverse Primer 5 DNA polymerase 1 Thermopol Buffer 5 dH2O 33 TOTAL 50μL
- 95°C,5min -> ( 95°C,1min -> 54°C,1min -> 72°C,1min )・・・25cycles -> 72°C,10min -> 6°C store
- --->Gel Check
Sample DNA : BBa_R0010
Sample DNA 1 Loading Dye 1 dH2O 4 TOTAL 6μl - R0010 --> 100μL
- Sample DNA:
1:BBa_R0010,2:BBa_S03154,3:BBa_J04500,4:BBa_C0161,5:BBa_C0261
Sample No. 1 2 3 4 5 Sample DNA 1 1 1 1 1 PstI 0.5 0.5 0.5 0.5 0.5 SpeI 0.5 - 1 - - XbaI - 0.5 - 0.5 0.5 Buffer 2 2 - 3 - - Buffer 3 - 6 - 6 6 (10×)BSA 2 0.6 3 - - (100×)BSA - - - 0.6 0.6 dH2O 3 6.4 7 1.4 1.4 TOTAL 20μl 60μl 30μl 60μl 60μl Team:Output
no work