Team:Hawaii/Notebook/2008-09-30

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Things we did today

Wetlab work

Plasmid Prep

Grace
  • Plasmid prep of BBpRL1383a #1, 3, 13, 18

Construction of secretion device (cont.)

Grace
  • PCR:
  • nir+rbs
  • slr1+GFPf
  • plac+rbs
  • rbs+GFP
  • nir
  • nir+rbs+pilA #17 (yet to be confirmed via sequencing)
  • rbs+GFPf+tt #1, 2 (yet to be confirmed via sequencing)
  • GFPf+tt #21
  • Overnight RE digest:
  • EcoRI/PstI:
  • pSB1A3
  • EcoRI/SpeI:
  • nir
  • rbs+GFP
  • nir+rbs
  • plac+rbs
  • slr1+GFPf
  • nir+rbs+pilA #17
  • XbaI/PstI:
  • slr1+GFPf
  • rbs+GFPf+tt #1, 2
  • GFPf+tt #21
  • rbs+GFP

Plasmid Prep

Margaret
  • pSB1A3, plac/rbs/rep + pSB1A3 (also made a glycerol stock of this), and oriT in pSB1A3

RE-digest from yesterday

Margaret
File:Gel purification 9-30.jpg
Gel purification of re-digest of aadaBB, aadapRL, and oriV.
  • gel purification of aadaBB, aadApRL, and oriV
  • de-phosphorylate pSB1A2, pSB1A3a, pSB3K3

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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