Team:KULeuven/19 August 2008

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Lab Work

Wet Lab

Electroporations with the following parts were performed: [http://partsregistry.org/Part:BBa_J23116 J23116]+[http://partsregistry.org/Part:BBa_B0032 B0032] , [http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:pSB1A2 pSB1A2], [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0032 B0032], [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022], [http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_B0032 B0032], [http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:BBa_B0015 B0015] and ([http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022])+([http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]). We also started a new ligation of [http://partsregistry.org/Part:BBa_K145151 K145151] with [http://partsregistry.org/Part:BBa_B0015 B0015].

We started the construction of the hybrid promotor [http://partsregistry.org/Part:BBa_K145150 K145150]:

An end-filling reaction was performed with Klenow polymerase and primers 1129/1130. This was followed by purification, but this failed. We couldn't see anything on the gel and the nanodrop confirmed this. Neverthless, a digest was made of the product - hopefully something will show up.
We also set up a second end-filling reaction (in case the first one fails).

Dry Lab

Modeling

Still looking into the diffusion of HSL. Redone TGS2 (The Grand Scheme aka Total Genetic System) as shown below to give all the latest parts/devices. We've also looked at the parts that we still need to order.

Wiki

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