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Team:KULeuven/31 August 2008
From 2008.igem.org
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Lab Work
Wet Lab
The electroporations of yesterday were a success. A liquid culture was made from [http://partsregistry.org/Part:BBa_R0011 R0011]+[http://partsregistry.org/Part:BBa_B0032 B0032], [http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015] and ([http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022])+([http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032]).
The XbaI digests of [http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_B0034 B0034], [http://partsregistry.org/Part:BBa_J23066 J23066], [http://partsregistry.org/Part:BBa_P0353 P0353], [http://partsregistry.org/Part:BBa_P0152 P0152], [http://partsregistry.org/Part:BBa_E0240 E0240], [http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015] were checked on a gel. Only C0040+B0015 and C0060+B0015 were no succes, they should lie higher then B0015, but they didn't (but we have a spare one with the new method). The XbaI digests of B0034, B0015, J23066, P0353, P1052 and E0240 were then further cut with EcoRI and put on a gel. These parts were cut out of the gel and are in the fridge now. See Gel 1.
[http://partsregistry.org/Part:BBa_C0056 C0056], [http://partsregistry.org/Part:BBa_R0053 R0053], [http://partsregistry.org/Part:BBa_C0061 C0061], [http://partsregistry.org/Part:BBa_B0014 B0014], [http://partsregistry.org/Part:BBa_E0040 E0040] were cut with EcoRI and SpeI and put on a gel. Only C0056, C0061 and E0040 were a succes (B0014 is probably to small to be used as an insert). These three were cut out and are in the fridge now. See Gel 2.
Two ligations were set for the night: [http://partsregistry.org/Part:BBa_C0056 C0056]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0061 C0061]+[http://partsregistry.org/Part:BBa_B0015 B0015].
