Team:KULeuven/3 September 2008

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  Input Output Filter InverTimer Reset Cell Death Memory

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Lab Work

Wet Lab

Once again, we tried to construct some of our new parts using PCR. It concerns the BioBricks K145014 (T7 polymerase with UmuD tag), K145150 (hybrid promoter) and K145013 (antisense LuxI).

We continued yesterday's digests. [http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015] were cut with EcoRI. They seemed alright.

We started a few ligations. This is step 2 of the ligation process, so they are a bit longer: (J23116+B0032)+(C0040+B0015), (R0040+B0032)+(K145015+B0015), (I712074+J23032)+(C0012+B0015), (I712074+J23032)+(C0060+B0015) and (R0040+J23022)+(J23109+J23032).

And finally, we also electroporated the ligations we made yesterday. Some of these ligations were electroporated into JM109 electrocompetent cells:

• into TOP10 -> [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_B0033 B0033], [http://partsregistry.org/Part:BBa_R0053 R0053]+[http://partsregistry.org/Part:BBa_P1052 P1052], [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23066 J23066], [http://partsregistry.org/Part:BBa_B0014 B0014]+[http://partsregistry.org/Part:BBa_B0033 B0033], [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_E0240 E0240], [http://partsregistry.org/Part:BBa_C0056 C0056]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0061 C0061]+[http://partsregistry.org/Part:BBa_B0015 B0015] - and of course pUC.
• into JM109 -> [http://partsregistry.org/Part:BBa_C0056 C0056]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0061 C0061]+[http://partsregistry.org/Part:BBa_B0015 B0015] - and pUC.

Dry Lab

Ethics... (re)-writing sections on the wiki... LaTeX output tool for MATLAB...

Modeling

Working out how we can make MATLAB remember diffusion of HSL in the medium during the simulation. Also investigating the scale at which this diffusion happens in the simulation

Remarks

Jonathan came to visit us. He's very interested in the iGEM competition and it was his first visit to a team. We had a very interesting conversation and it was a really inspiring visit.

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