Team:Rice University/Notebook/21 May 2008

From 2008.igem.org

Wednesday 21 May

  • Taylor Stevenson
    • Transformation From New Registry (Paper Punches)-Electroporation of [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13602 BBa_I13602] located on [http://partsregistry.org/Part:pSB1A2 pSB1A2] into TetR electrocompetent cells.
    1. Added 2uL of DNA solution obtained by soaking DNA punch in (10:1) Tris:EDTA buffer incubated @ 42*C for 20min.
    2. Electropulse parameters: 1.8 kV & 5.4 ms
    3. Plated 100uL and 20uL of transformed cells as well as 20uL of 1/100 and 1/10,000 dilutions in sterile 50% glycerol. Plates were cultured O/N.
    • Result-no colonies were observed at any dilution






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