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Alberta NINT/11 July 2008
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Measured cell count using spectrophotometer of 10/07/08 overnights. | Measured cell count using spectrophotometer of 10/07/08 overnights. | ||
Lac Z Activity Assay was unsuccessful - perhaps not enough cells were used? | Lac Z Activity Assay was unsuccessful - perhaps not enough cells were used? | ||
| + | |||
| + | lab work (JD): | ||
| + | Carried out Day 2 protocol for Activity Assay | ||
| + | Like stated above, was not successful. | ||
| + | Collected quantitative data of restriction fragments for ligation | ||
| + | Ligated K102003+pSB4A5=K102031 and K102006+pSB4A5=K102021 | ||
| + | Transformed XL1-B Cells | ||
| + | Plated cells on LB + Amp50 | ||
[[Alberta_NINT/14_July_2008 | Next entry >]] | [[Alberta_NINT/14_July_2008 | Next entry >]] | ||
Revision as of 17:44, 21 July 2008
lab work (SD):
Measured cell count using spectrophotometer of 10/07/08 overnights.
Lac Z Activity Assay was unsuccessful - perhaps not enough cells were used?
lab work (JD):
Carried out Day 2 protocol for Activity Assay
Like stated above, was not successful.
Collected quantitative data of restriction fragments for ligation
Ligated K102003+pSB4A5=K102031 and K102006+pSB4A5=K102021
Transformed XL1-B Cells
Plated cells on LB + Amp50
