Team:KULeuven/Project/Output

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Revision as of 15:19, 30 July 2008

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Output

We have been searching for an easy accesible output system, that could us provide in a quick and easy way qualitative and quantitative information. The choice for GFP was quickly made.

BioBricks

Components

We added C-terminal a LVA tag for rapid degradation of the protein, so that we can follow the output signal quickly in time. The OmpF promoter (http://partsregistry.org/Part:BBa_R0084 BBa_R0084]) behaves as described in the input section. For fine-tuning reasons, the RBS was chosen [http://partsregistry.org/Part:BBa_B0032 BBa_B0032] with a relative efficiency of 0,3.

Action

This is a very easy system. As the input results in a low concentration of OmpR, there is GFP production, proportional to the input signal.

PI remarks

@@ Line 4: / Line 4: @@
 ==Output==
+We have been searching for an easy accesible output system, that could us provide in a quick and easy way qualitative and quantitative information. The choice for GFP was quickly made.
+===BioBricks===
 [[Image:Output_BioBrick.jpg|center]]
-We have been searching for an easy accesible output system, that could us provide in a quick and easy way qualitative and quantitative information. The choice for GFP was quickly made. We added an LVA C-terminal tag for rapid degradation of the protein, so that we can follow the output signal quickly in time. More information about this type of tagging can be found here ('''link''').
+===Components===
+We added C-terminal a [https://2008.igem.org/Team:KULeuven/Literature#LVA_tag LVA tag] for rapid degradation of the protein, so that we can follow the output signal quickly in time. The OmpF promoter (http://partsregistry.org/Part:BBa_R0084 '''BBa_R0084''']) behaves as described in the [https://2008.igem.org/Team:KULeuven/Project/Input input] section. For fine-tuning reasons, the RBS was chosen [http://partsregistry.org/Part:BBa_B0032 '''BBa_B0032'''] with a relative efficiency of 0,3.
-The OmpF promoter ('''BBa_R0084''') behaves as described in the input section. For fine-tuning reasons, the RBS was chosen '''BBa_B0032''' with a relative efficiency of 0,3.
+===Action===
+This is a very easy system. As the [https://2008.igem.org/Team:KULeuven/Project/Input input] results in a low concentration of OmpR, there is GFP production, proportional to the input signal.
+'''PI remarks'''