Team:KULeuven/Project/Output

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This is a very easy system. As the [https://2008.igem.org/Team:KULeuven/Project/Input input] results in a low concentration of phosphorylated OmpR, there is GFP production, proportional to the input signal.
This is a very easy system. As the [https://2008.igem.org/Team:KULeuven/Project/Input input] results in a low concentration of phosphorylated OmpR, there is GFP production, proportional to the input signal.
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==References==
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Appl Environ Microbiol. 1998 Jun;64(6):2240-6.
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New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria.
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Andersen JB, Sternberg C, Poulsen LK, Bjorn SP, Givskov M, Molin S.
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PMID: 9603842
{{:Team:KULeuven/Tools/Components}}
{{:Team:KULeuven/Tools/Components}}

Revision as of 08:30, 25 August 2008

  dock/undock dropdown  

Pictogram output.png

Contents

Output

GFP.png

We have been searching for an easy accessible output system, that could provide us qualitative and quantitative information in a quick and easy way. The choice for GFP was easily made.

BioBricks

Project output.jpg

Components

We added C-terminal a LVA tag for rapid degradation of the protein, so that we can follow the output signal quickly in time. The OmpF promoter ([http://partsregistry.org/Part:BBa_R0084 BBa_R0084]) behaves as described in the input section. For fine-tuning reasons, the RBS was chosen [http://partsregistry.org/Part:BBa_B0032 BBa_B0032] with a relative efficiency of 0,3.

Action

This is a very easy system. As the input results in a low concentration of phosphorylated OmpR, there is GFP production, proportional to the input signal.

References

Appl Environ Microbiol. 1998 Jun;64(6):2240-6.
New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria.
Andersen JB, Sternberg C, Poulsen LK, Bjorn SP, Givskov M, Molin S.
PMID: 9603842



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