Team:Paris/Notebook/Freezer

From 2008.igem.org

(Difference between revisions)
(→Ligation: Construction realised)
(→Ligation: Construction realised)
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== Ligation: Construction realised ==
+
== Ligations: Constructions realised ==
{|
{|
|- style="background: #649CD7;"  
|- style="background: #649CD7;"  
! colspan="8" style="background: #649CD7;" | Plasmids: MiniPrep Products 2008
! colspan="8" style="background: #649CD7;" | Plasmids: MiniPrep Products 2008
|- style="background: #649CD7; text-align: center;"
|- style="background: #649CD7; text-align: center;"
-
|width=15%| Number
+
|width=7,5%| Number
-
|width=15%| Plasmid
+
|width=7,5%| Plasmid
-
|width=20%| Biobricks
+
|width=22,5%| Biobricks
-
|width=20%| Description
+
|width=22,5%| Description
-
|width=10%| Digestion
+
|width=20%| Digestion
-
|width=5%| comment
+
|width=7,5%| comment
-
|width=15%| Date
+
|width=7,5%| Date
-
|-style="background: #D4E2EF;" |L102
+
|- style="background: #dddddd;" 
 +
|style="background: #D4E2EF;" |L102
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]
Line 403: Line 404:
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]
-
|align="center"|Strong rbs - lasR activator<br>D129 (BV) -  D114 (BI)
+
|align="center"|Strong rbs - lasR activator
-
|
+
|align="center"|D129 (BV) -  D114 (BI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
Line 411: Line 412:
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
-
|align="center"|Strong promoter - ECFP<br>D123 (BV) - D130 (BI)
+
|align="center"|Strong promoter - ECFP
-
|
+
|align="center"|D123 (BV) - D130 (BI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
Line 419: Line 420:
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
-
|align="center"|Strong promoter - gfp Tripart<br>D123 (BV) - D131 (BI)
+
|align="center"|Strong promoter - gfp Tripart
-
|
+
|align="center"|D123 (BV) - D131 (BI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
Line 427: Line 428:
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
-
|align="center"|Strong promoter - ECFP<br>D103 (BV) - D130 (BI)
+
|align="center"|Strong promoter - ECFP
-
|
+
|align="center"|D103 (BV) - D130 (BI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
Line 435: Line 436:
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
-
|align="center"|Strong promoter -  gfp Tripart<br>D103 (BV) - D131 (BI)
+
|align="center"|Strong promoter -  gfp Tripart
-
|
+
|align="center"|D103 (BV) - D131 (BI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
Line 443: Line 444:
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
-
|align="center"|Medium promoter -  gfp Tripart<br>D124 (BV) - D131 (BI)
+
|align="center"|Medium promoter -  gfp Tripart
-
|
+
|align="center"|D124 (BV) - D131 (BI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
Line 451: Line 452:
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
-
|align="center"|Weak promoter - ECFP<br>D104 (BV) - D130 (BI)
+
|align="center"|Weak promoter - ECFP
-
|
+
|align="center"|D104 (BV) - D130 (BI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
Line 459: Line 460:
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
-
|align="center"|Weak promoter - GFP tripart<br>D104 (BV) - D131 (BI)
+
|align="center"|Weak promoter - GFP tripart
-
|
+
|align="center"|D104 (BV) - D131 (BI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
Line 467: Line 468:
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
-
|align="center"|pLas - ECFP<br>D105 (BV) - D130 (BI)
+
|align="center"|pLas - ECFP
-
|
+
|align="center"|D105 (BV) - D130 (BI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
Line 475: Line 476:
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
-
|align="center"|pLas - gfp Tripart<br>D105 (BV) - D131 (BI)
+
|align="center"|pLas - gfp Tripart
-
|
+
|align="center"|D105 (BV) - D131 (BI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
Line 483: Line 484:
|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
|align="center"|[[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
-
|align="center"|yfp - Double Terminator<br>D125 (FV) - D117 (FI)
+
|align="center"|yfp - Double Terminator
-
|
+
|align="center"|D125 (FV) - D117 (FI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
Line 491: Line 492:
|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
|align="center"|[[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
-
|align="center"|rfp - Double Terminator<br>D125 (FV) - D121 (FI)
+
|align="center"|rfp - Double Terminator
-
|
+
|align="center"|D125 (FV) - D121 (FI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
Line 499: Line 500:
|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
|align="center"|[[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
-
|align="center"|lasR activator + LVA - Double Terminator<br>D125 (FV) - D113 (FI)
+
|align="center"|lasR activator + LVA - Double Terminator
-
|
+
|align="center"|D125 (FV) - D113 (FI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
Line 507: Line 508:
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
-
|align="center"|pTet - gfp tripart<br>D106 (BV) - D131 (BI)
+
|align="center"|pTet - gfp tripart
-
|
+
|align="center"|D106 (BV) - D131 (BI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
Line 515: Line 516:
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]
-
|align="center"|Strongest RBS - rfp<br>D102 (BV) - D122 (BI)
+
|align="center"|Strongest RBS - rfp
-
|
+
|align="center"|D102 (BV) - D122 (BI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
Line 523: Line 524:
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]]
-
|align="center"|Strongest RBS - yfp<br>D102 (BV) - D118 (BI)
+
|align="center"|Strongest RBS - yfp
-
|
+
|align="center"|D102 (BV) - D118 (BI)
|
|
|align="center"|[[Team:Paris/August 1|August 1]]
|align="center"|[[Team:Paris/August 1|August 1]]
Line 531: Line 532:
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
-
|align="center"|pFlgA-RFP<br>D132 (FI) -  D136 (FV)
+
|align="center"|pFlgA-RFP
-
|
+
|align="center"|D132 (FI) -  D136 (FV)
|
|
|align="center"|[[Team:Paris/August 12|August 12]]
|align="center"|[[Team:Paris/August 12|August 12]]
Line 539: Line 540:
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
-
|align="center"|pFlgB-RFP<br>D133 (FI) -  D136 (FV)
+
|align="center"|pFlgB-RFP
-
|
+
|align="center"|D133 (FI) -  D136 (FV)
|
|
|align="center"|[[Team:Paris/August 12|August 12]]
|align="center"|[[Team:Paris/August 12|August 12]]
Line 547: Line 548:
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|[http://partsregistry.org/Part:J61002 J61002]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_reporter.png]]
-
|align="center"|pFlhB-RFP<br>D134 (FI) -  D136 (FV)
+
|align="center"|pFlhB-RFP
-
|
+
|align="center"|D134 (FI) -  D136 (FV)
|
|
|align="center"|[[Team:Paris/August 12|August 12]]
|align="center"|[[Team:Paris/August 12|August 12]]
Line 555: Line 556:
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
-
|align="center"|rbs TetR - ECFP<br>D110 (BV) -  D130 (BI)
+
|align="center"|rbs TetR - ECFP
-
|
+
|align="center"|D110 (BV) -  D130 (BI)
|
|
|align="center"|[[Team:Paris/August 13|August 13]]
|align="center"|[[Team:Paris/August 13|August 13]]
Line 563: Line 564:
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
-
|align="center"|rbs TetR - GFP tripart<br>D110 (BV) -  D131 (BI) DO NOT WORK !!!!
+
|align="center"|rbs TetR - GFP tripart
-
|
+
|align="center"|D110 (BV) -  D131 (BI)  
-
|
+
|align="center"|DO NOT WORK !!!!
|align="center"|[[Team:Paris/August 13|August 13]]
|align="center"|[[Team:Paris/August 13|August 13]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 571: Line 572:
|align="center" |[http://partsregistry.org/Part:pSB2K3 pSB2K3]
|align="center" |[http://partsregistry.org/Part:pSB2K3 pSB2K3]
|align="center"| [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
|align="center"| [[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
-
|align="center"|AracpBAD - gfp tripart<br>D126 (BV) - D131 (BI)
+
|align="center"|AracpBAD - gfp tripart
-
|
+
|align="center"|D126 (BV) - D131 (BI)
|
|
|align="center"|[[Team:Paris/August 13|August 13]]
|align="center"|[[Team:Paris/August 13|August 13]]
Line 579: Line 580:
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Part_icon_regulatory.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
-
|align="center"|tetR repressible promoter - ECFP<br>D106 (BV) - D130 (BI)
+
|align="center"|tetR repressible promoter - ECFP
-
|
+
|align="center"|D106 (BV) - D130 (BI)
|
|
|align="center"|[[Team:Paris/August 13|August 13]]
|align="center"|[[Team:Paris/August 13|August 13]]
Line 587: Line 588:
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
-
|align="center"|RBS-lasI - ECFP <br>D107 (BV) - D130 (BI)
+
|align="center"|RBS-lasI - ECFP  
-
|
+
|align="center"|D107 (BV) - D130 (BI)
|
|
|align="center"|[[Team:Paris/August 13|August 13]]
|align="center"|[[Team:Paris/August 13|August 13]]
Line 595: Line 596:
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_rbs.png]][[Image:Part_icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
-
|align="center"|RBS lasI - gfp tripart<br>D107 (BV) - D131 (BI)
+
|align="center"|RBS lasI - gfp tripart
-
|
+
|align="center"|D107 (BV) - D131 (BI)
|
|
|align="center"|[[Team:Paris/August 13|August 13]]
|align="center"|[[Team:Paris/August 13|August 13]]
Line 603: Line 604:
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]
-
|align="center"|Strongest RBS (1)- LasR activator (+LVA)<br>D102 (BV) - D114 (BI)
+
|align="center"|Strongest RBS (1)- LasR activator (+LVA)
-
|
+
|align="center"|D102 (BV) - D114 (BI)
|
|
|align="center"|[[Team:Paris/August 13|August 13]]
|align="center"|[[Team:Paris/August 13|August 13]]
Line 611: Line 612:
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[[Image:Icon_coding.png]]
|align="center"|[[Image:Icon_coding.png]]
-
|align="center"|flhDC <br> D142(FV) - D139(FI)  
+
|align="center"|flhDC  
-
|
+
|align="center"|D142(FV) - D139(FI)  
|
|
|align="center"|[[Team:Paris/August 13|August 13]]
|align="center"|[[Team:Paris/August 13|August 13]]
Line 619: Line 620:
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[[Image:Icon_coding.png]]
|align="center"|[[Image:Icon_coding.png]]
-
|align="center"|OmpR*<br> D142(FV) - D140(FI)
+
|align="center"|OmpR*
 +
|align="center"|D142(FV) - D140(FI)
|WT sequence (August 19)
|WT sequence (August 19)
-
|
 
|align="center"|[[Team:Paris/August 13|August 13]] ; [[Team:Paris/August 19|August 19]]
|align="center"|[[Team:Paris/August 13|August 13]] ; [[Team:Paris/August 19|August 19]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 627: Line 628:
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[[Image:Icon_coding.png]]
|align="center"|[[Image:Icon_coding.png]]
-
|align="center"| EnvZ*<br> D142(FV) - D141(FI)
+
|align="center"| EnvZ*
 +
|align="center"| D142(FV) - D141(FI)
|lacks about 300 bp in EnvZ* (August 19)
|lacks about 300 bp in EnvZ* (August 19)
-
|
 
|align="center"|[[Team:Paris/August 13| August 13]] [[Team:Paris/August 19| August 19]]
|align="center"|[[Team:Paris/August 13| August 13]] [[Team:Paris/August 19| August 19]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 635: Line 636:
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
-
|align="center"|gfp generator (E0240)<br> D137(FV) - D138(FI)
+
|align="center"|gfp generator (E0240)
-
|
+
|align="center"| D137(FV) - D138(FI)
|
|
|align="center"|[[Team:Paris/August 13|August 13]]
|align="center"|[[Team:Paris/August 13|August 13]]
Line 643: Line 644:
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
|align="center"|[http://partsregistry.org/Part:pSB3K3 pSB3K3]
|align="center"|
|align="center"|
-
|align="center"|E0240 RBS+<br> D140 (PstI) - D144 (ES)
+
|align="center"|E0240 RBS+
 +
|align="center"|D140 (PstI) - D144 (ES)
|seems to have the plasmid without the insert and one with the insert
|seems to have the plasmid without the insert and one with the insert
-
|
 
|align="center"|[[Team:Paris/August 15|August 15]]
|align="center"|[[Team:Paris/August 15|August 15]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 651: Line 652:
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|
|align="center"|
-
|align="center"|flhD<br> D142 (ES) - D145 (ES)
+
|align="center"|flhD
 +
|align="center"| D142 (ES) - D145 (ES)
|has not been sequenced
|has not been sequenced
-
|
 
|align="center"|[[Team:Paris/August 15|August 15]]
|align="center"|[[Team:Paris/August 15|August 15]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 659: Line 660:
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|
|align="center"|
-
|align="center"|flhDC+promotor<br> D143 (ES) - D145 (ES)
+
|align="center"|flhDC+promotor
 +
|align="center"| D143 (ES) - D145 (ES)
|does not have the insert
|does not have the insert
-
|
 
|align="center"|[[Team:Paris/August 15|August 15]]
|align="center"|[[Team:Paris/August 15|August 15]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 667: Line 668:
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Icon_regulatory.png]][[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
-
|align="center"|pLas + rbs-tetR-gfp tripart<br> D105 (SP) - D146 (XP)
+
|align="center"|pLas + rbs-tetR-gfp tripart
 +
|align="center"|D105 (SP) - D146 (XP)
|incorrect clone -> to do again
|incorrect clone -> to do again
-
|
 
|align="center"|[[Team:Paris/August 19|August 19]]
|align="center"|[[Team:Paris/August 19|August 19]]
|- style="background: #dddddd;"   
|- style="background: #dddddd;"   
Line 675: Line 676:
|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
|align="center"|[http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
|align="center"|[[Image:Icon_rbs.png]][[Image:Icon_coding.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]]
-
|align="center"|rbs-lasR + double terminator<br> D147 (ES) - D125 (EX)
+
|align="center"|rbs-lasR + double terminator
-
|
+
|align="center"| D147 (ES) - D125 (EX)
|
|
|align="center"|[[Team:Paris/August 19|August 19]]
|align="center"|[[Team:Paris/August 19|August 19]]
Line 682: Line 683:
| style="background: #D4E2EF;" |L167
| style="background: #D4E2EF;" |L167
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-
|align="center"|gfp generator(E0240) - pTet <br>[[Image:Part_icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_regulatory.png]]
+
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_reporter.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_terminator.png]][[Image:Part_icon_regulatory.png]]
|align="center"|gfp generator(E0240) - pTet
|align="center"|gfp generator(E0240) - pTet
|
|
Line 690: Line 691:
| style="background: #D4E2EF;" |L166
| style="background: #D4E2EF;" |L166
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
|align="center"|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
-
|align="center"|RBS(B0032) - tetR<br>[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]
+
|align="center"|[[Image:Part_icon_rbs.png]][[Image:Icon_coding.png]]
|align="center"|RBS(B0032) - tetR
|align="center"|RBS(B0032) - tetR
|
|

Revision as of 20:17, 17 September 2008

Contents

Last number

(check on 11 sept 2008, subject to change by unwritten data on the wiki !! FC)

Last Ligation number used
185


Last PCR number used
154


Last Digestion number used
202


Last Miniprep number used
172


Last Strain number used
168

PCR products

PCR products
Number Name Oligo F Oligo R Template Length Comments Product Left ? [DNA]µg/mL Date
PCR124 pFlgA O100 O101 - 261pb yes 0.5 August 7
PCR125 pFlgB O102 O103 - 261pb yes 0.5 August 7
PCR126 pFlhB O108 O109 - 260pb yes 0.5 August 7
PCR127 flhDC O132 O131 MG1655 no August 8
PCR128 OmpR* O138 O139 Strain OmpR* 700pb yes 150 August 8
PCR129 EnvZ* O126 O127 Strain EnvZ* 1400pb yes 100 August 8
PCR130 E0240 rbs+ O141 O140 MP143 900pb no August 11
PCR131 flhD rbs- O132 O133 MG1655 350pb no August 11
PCR132 pFlhD rbs- O130 O131 MG1655 600pb no August 11
PCR133 flhDC + prom O110 O131 MG1655 1300pb no August 11
PCR134 flhDC + prom O111 O131 MG1655 no August 11
PCR135 pfliL O124 O125 MG1655 yes August 11
PCR136 pFlhDC O110 O113 MG1655 yes August 11
PCR137 pFlhDC O 111 O 113 MG1655 yes August 11
PCR138 FlhDC (RBS -) O 132 O 131 MG 1655 yes August 15
PCR139 pflhDC (URI) O 110 O 113 PCR 130 yes August 15
PCR140 flhDC (RBS -) O 132 O 131 PCR 130 yes August 15
PCR141 Gene FlhDC + rbs nat O 134 O 131 MG1655 1000pb yes August 26
PCR142 pFliA + rbs nat O 145 O 144 MG1655 325pb yes August 26
PCR143 pFliA sans rbs O 145 O 146 MG1655 310pb yes August 26
PCR144 pFliA + Gene FliA O 145 O 151 MG1655 1100pb yes August 26
PCR145 PCRFliA1 O 148 O 152 MG1655 150pb yes August 26
PCR146 PCRFliA2 O 153 O 150 MG1655 670pb yes August 26
PCR 147 EnvZ* O 126 O 127 S 120 1412 bp yes 150 August 26
PCR 148 OmpR* O 138 O 139 S 119 789 bp yes 101 August 26
PCR 151 pFLiA without nat. rbs O 145 O 146 MG1655 310 bp yes 34 August 26
PCR 152 pFliA rbs nat. O 145 O 144 MG1655 325 bp yes 19 August 26
PCR 153 pFliA+Gene FliA non-mutated O 145 O 151 MG1655 1100 bp yes 39 August 26
PCR 154 Gene FliA mutated (EcoRI/PstI) O 148 O 150 MG1655 800 bp 154.1, 154.2, 154.3 yes 50 August 26

Ligations: Constructions realised

Plasmids: MiniPrep Products 2008
Number Plasmid Biobricks Description Digestion comment Date
L102 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.png Strong rbs - YFP D129 (BV) - D118 (BI) August 1
L103 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.png Strong rbs - mRFP D129 (BV) - D122 (BI) August 1
L104 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.png Strong rbs - lasR activator D129 (BV) - D114 (BI) August 1
L105 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Strong promoter - ECFP D123 (BV) - D130 (BI) August 1
L106 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Strong promoter - gfp Tripart D123 (BV) - D131 (BI) August 1
L107 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Strong promoter - ECFP D103 (BV) - D130 (BI) August 1
L108 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Strong promoter - gfp Tripart D103 (BV) - D131 (BI) August 1
L110 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Medium promoter - gfp Tripart D124 (BV) - D131 (BI) August 1
L111 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Weak promoter - ECFP D104 (BV) - D130 (BI) August 1
L112 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Weak promoter - GFP tripart D104 (BV) - D131 (BI) August 1
L115 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png pLas - ECFP D105 (BV) - D130 (BI) August 1
L116 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png pLas - gfp Tripart D105 (BV) - D131 (BI) August 1
L117 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Part icon reporter.pngPart icon terminator.pngPart icon terminator.png yfp - Double Terminator D125 (FV) - D117 (FI) August 1
L118 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Part icon reporter.pngPart icon terminator.pngPart icon terminator.png rfp - Double Terminator D125 (FV) - D121 (FI) August 1
L119 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Icon coding.pngPart icon terminator.pngPart icon terminator.png lasR activator + LVA - Double Terminator D125 (FV) - D113 (FI) August 1
L121 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png pTet - gfp tripart D106 (BV) - D131 (BI) August 1
L124 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.png Strongest RBS - rfp D102 (BV) - D122 (BI) August 1
L125 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.png Strongest RBS - yfp D102 (BV) - D118 (BI) August 1
L128 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon reporter.png pFlgA-RFP D132 (FI) - D136 (FV) August 12
L129 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon reporter.png pFlgB-RFP D133 (FI) - D136 (FV) August 12
L130 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon reporter.png pFlhB-RFP D134 (FI) - D136 (FV) August 12
L100 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png rbs TetR - ECFP D110 (BV) - D130 (BI) August 13
L101 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png rbs TetR - GFP tripart D110 (BV) - D131 (BI) DO NOT WORK !!!! August 13
L114 [http://partsregistry.org/Part:pSB2K3 pSB2K3] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png AracpBAD - gfp tripart D126 (BV) - D131 (BI) August 13
L120 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png tetR repressible promoter - ECFP D106 (BV) - D130 (BI) August 13
L122 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png RBS-lasI - ECFP D107 (BV) - D130 (BI) August 13
L123 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png RBS lasI - gfp tripart D107 (BV) - D131 (BI) August 13
L126 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.png Strongest RBS (1)- LasR activator (+LVA) D102 (BV) - D114 (BI) August 13
L132 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png flhDC D142(FV) - D139(FI) August 13
L133 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png OmpR* D142(FV) - D140(FI) WT sequence (August 19) August 13 ; August 19
L134 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png EnvZ* D142(FV) - D141(FI) lacks about 300 bp in EnvZ* (August 19) August 13 August 19
L138 [http://partsregistry.org/Part:pSB3K3 pSB3K3] Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png gfp generator (E0240) D137(FV) - D138(FI) August 13
L139 [http://partsregistry.org/Part:pSB3K3 pSB3K3] E0240 RBS+ D140 (PstI) - D144 (ES) seems to have the plasmid without the insert and one with the insert August 15
L141 [http://partsregistry.org/Part:pSB1A2 pSB1A2] flhD D142 (ES) - D145 (ES) has not been sequenced August 15
L142 [http://partsregistry.org/Part:pSB1A2 pSB1A2] flhDC+promotor D143 (ES) - D145 (ES) does not have the insert August 15
L150 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon regulatory.pngIcon rbs.pngIcon coding.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png pLas + rbs-tetR-gfp tripart D105 (SP) - D146 (XP) incorrect clone -> to do again August 19
L151 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Icon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png rbs-lasR + double terminator D147 (ES) - D125 (EX) August 19
L167 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.pngPart icon regulatory.png gfp generator(E0240) - pTet August 25
L166 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.png RBS(B0032) - tetR August 25

Plasmids: MiniPrep Products

Plasmids: MiniPrep Products 2008
Number Name Plamsid Biobricks Description Products in use/comments Conc. ug/mL Date
MP100 [http://partsregistry.org/Part:BBa_B0034 B0034 ] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.png Strongest RBS (Efficiency = 1) July 26
MP101.1 [http://partsregistry.org/Part:BBa_J23101 J23101] [http://partsregistry.org/Part:BBa_J61002 pJ61002 ] Part icon regulatory.png Strongest Constitutive promoter July 26 333 July 22, July 23, July 26
MP101.2 finished
MP101.4 200
MP102 [http://partsregistry.org/Part:BBa_J23109 J23109] [http://partsregistry.org/Part:BBa_J61002 pJ61002] Part icon regulatory.png Weak Constitutive promoter July 26 July 22, July 26
MP103 [http://partsregistry.org/Part:BBa_R0079 R0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.png promoter pLas (lasr & PAI regulated) July 26 July 23, July 26
MP104.1 [http://partsregistry.org/Part:BBa_R0040 R0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.png tetR repressible promoter July 26 145 July 23, July 26
MP104.3 147
MP104.4 51
MP105.1 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.png B0034(rbs) ->LasI 34 July 26
MP105.2 31
MP106.1 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.png B0034(rbs) -> TetR 80 August 24
MP106.3 40
MP107 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] Icon coding.png lasR activator with LVA Tag July 23
MP108 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png lasR activator July 26 July 22, July 23, July 26
MP109 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Part icon reporter.png YFP July 26
MP110 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon reporter.png GFP July 26 July 23, July 26
MP111 [http://partsregistry.org/Part:BBa_E1010 E1010 ] [http://partsregistry.org/Part:pSB2K3 pSB2K3] Part icon reporter.png mRFP July 26 July 23, July 26
MP112 OmpR M57I Icon coding.png July 23
MP113 EnvZ I281S Icon coding.png July 23
MP114.1 [http://partsregistry.org/Part:BBa_C0040 C0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png tetR repressor 173 July 23
MP114.2 263
MP115 [http://partsregistry.org/Part:pSB1A3 pSB1A3] Icon plasmid.png July 23
MP116 [http://partsregistry.org/Part:BBa_J23100 pJ23100] [http://partsregistry.org/Part:BBa_J61002 J61002] Part icon regulatory.png Strong constitutive promoter in J61002 July 26
MP117 [http://partsregistry.org/Part:BBa_J23107 pJ23107] [http://partsregistry.org/Part:BBa_J61002 J61002] Part icon regulatory.png Medium constitutive promoter in J61002 July 26 July 24, July 26
MP118.1 [http://partsregistry.org/Part:BBa_B0015 B0015] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Part icon terminator.pngPart icon terminator.png Double terminator July 26 220 July 24, July 26
MP118.3 187
MP119.1 [http://partsregistry.org/Part:BBa_I0500 I0500] [http://partsregistry.org/Part:pSB2K3 pSB2K3] Part icon regulatory.png AraC pBAD July 26 42 July 24, July 26
MP119.2 26
MP119.3 39
MP120 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.png Strong RBS (Efficiency = 0,6) July 26
MP121 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png ECFP (RBS+LVA+Double Term) July 26 July 24, July 26
MP122 [http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png gfp tri-part; strong rbs July 26
MP123 [http://partsregistry.org/Part:J61002 J61002] Icon plasmid.png August 5
MP124 L102 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.png Strong rbs - YFP
D129 (BV) - D118 (BI) 		August 1
MP125 L103 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.png Strong rbs - mRFP
D129 (BV) - D122 (BI) 		August 1
MP126 L104 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.png Strong rbs - lasR activator
D129 (BV) - D114 (BI) 		August 1
MP127 L105 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Strong promoter - ECFP
D123 (BV) - D130 (BI) 		August 1
MP128 L106 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Strong promoter - gfp Tripart
D123 (BV) - D131 (BI) 		August 1
MP129 L107 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Strong promoter - ECFP
D103 (BV) - D130 (BI) 		August 1
MP130 L108 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Strong promoter - gfp Tripart
D103 (BV) - D131 (BI) 		August 1
MP131 L110 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Medium promoter - gfp Tripart
D124 (BV) - D131 (BI) 		August 1
MP132 L111 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Weak promoter - ECFP
D104 (BV) - D130 (BI) 		August 1
MP133 L112 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Weak promoter - GFP tripart
D104 (BV) - D131 (BI) 		August 1
MP134 L115 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png pLas - ECFP
D105 (BV) - D130 (BI) 		August 1
MP135 L116 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png pLas - gfp Tripart
D105 (BV) - D131 (BI) 		August 1
MP136 L117 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Part icon reporter.pngPart icon terminator.pngPart icon terminator.png yfp - Double Terminator
D125 (FV) - D117 (FI) 		August 1
MP137 L118 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Part icon reporter.pngPart icon terminator.pngPart icon terminator.png rfp - Double Terminator
D125 (FV) - D121 (FI) 		August 1
MP138 L119 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Icon coding.pngPart icon terminator.pngPart icon terminator.png lasR activator + LVA - Double Terminator
D125 (FV) - D113 (FI) 		August 1
MP139 L121 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png pTet - gfp tripart
D106 (BV) - D131 (BI) 		August 1
MP140 L124 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.png Strongest RBS - rfp
D102 (BV) - D122 (BI) 		August 1
MP141 L125 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.png Strongest RBS - yfp
D102 (BV) - D118 (BI) 		August 1
MP142 [http://partsregistry.org/Part:pSB3K3 pSB3K3] Icon plasmid.png August 8
MP143.1 [http://partsregistry.org/Part:BBa_E0240 E0240] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png gfp generator 138 August 8
MP143.2 150
MP144.1 L128 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon reporter.png pFlgA-RFP
D132 (FI) - D136 (FV) 		MP144.3 ; MP144.4 127 August 12
MP144.2 166
MP144.3 163
MP144.4 154
MP145.1 L129 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon reporter.png pFlgB-RFP
D133 (FI) - D136 (FV) 		MP145.6 ; MP145.7 136 August 12
MP145.2 151
MP145.6 163
MP145.7 188
MP146.1 L130 [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon reporter.png pFlhB-RFP
D134 (FI) - D136 (FV) 		MP146.1 ; MP146.2 298 August 12
MP146.2 142
MP146.7 170
MP146.8 143
MP147 L100 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png rbs TetR - ECFP
D110 (BV) - D130 (BI) 		August 13
MP148.1 L101 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png rbs TetR - GFP tripart
D110 (BV) - D131 (BI) DO NOT WORK !!!! 		MP148.2 ; MP148.3 109 August 13
MP148.2 123
MP148.3 123
MP149 L114 [http://partsregistry.org/Part:pSB2K3 pSB2K3] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png AracpBAD - gfp tripart
D126 (BV) - D131 (BI) 		August 13
MP150 L120 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png tetR repressible promoter - ECFP
D106 (BV) - D130 (BI) 		August 13
MP151 L122 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png RBS-lasI - ECFP
D107 (BV) - D130 (BI) 		August 13
MP152 L123 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png RBS lasI - gfp tripart
D107 (BV) - D131 (BI) 		August 13
MP153.1 L126 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.png Strongest RBS (1)- LasR activator (+LVA)
D102 (BV) - D114 (BI) 		MP153.1 ; MP153.3 100 August 13
MP153.2 131
MP153.3 113
MP154 L132 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png flhDC
D142(FV) - D139(FI) 		August 13
MP155 L133 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png OmpR*
D142(FV) - D140(FI) 		WT sequence (August 19) August 13 ; August 19
MP156 L134 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png EnvZ*
D142(FV) - D141(FI) 		lacks about 300 bp in EnvZ* (August 19) August 13 August 19
MP157 L138 [http://partsregistry.org/Part:pSB3K3 pSB3K3] Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png gfp generator (E0240)
D137(FV) - D138(FI) 		August 13
MP158 L139 [http://partsregistry.org/Part:pSB3K3 pSB3K3] E0240 RBS+
D140 (PstI) - D144 (ES) 		seems to have the plasmid without the insert and one with the insert August 15
MP159 L141 [http://partsregistry.org/Part:pSB1A2 pSB1A2] flhD
D142 (ES) - D145 (ES) 		has not been sequenced August 15
MP160 L142 [http://partsregistry.org/Part:pSB1A2 pSB1A2] flhDC+promotor
D143 (ES) - D145 (ES) 		does not have the insert August 15
MP161 L150 [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon regulatory.pngIcon rbs.pngIcon coding.pngIcon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png pLas + rbs-tetR-gfp tripart
D105 (SP) - D146 (XP) 		incorrect clone -> to do again August 19
MP162.1 L151.1 [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Icon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png rbs-lasR + double terminator
D147 (ES) - D125 (EX) 		151 August 19
MP162.2 172
MP162.3 149
MP163.1 [http://partsregistry.org/Part:BBa_B0032 B0032] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon rbs.png RBS 80 August 20
MP163.2 79
MP164 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png RBS+ ECFP+ LVA+ term August 20
MP165 [http://partsregistry.org/Part:BBa_E0430 E0430] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png RBS+ YFP+ LVA- term August 20
MP166 [http://partsregistry.org/Part:BBa_E0432 E0432] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png RBS+ YFP LVA+ term BUT be careful , it doesn't work. See registry August 20
MP167 [http://partsregistry.org/Part:BBa_E0420 E0420] [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png RBS+ ECFP LVA- term ceci ne contient pas de LVA apparemment. ça correspondrait au ECFP normal de longue durée de vie August 20
MP168 [http://partsregistry.org/Part:BBa_I732078 I732078] [http://partsregistry.org/Part:pSB1A3 pSB1A3] Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png RBS+ mRFP LVA+ term August 20
MP171 L167 [http://partsregistry.org/Part:pSB1A2 pSB1A2] gfp generator(E0240) - pTet
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.pngPart icon regulatory.png 		gfp generator(E0240) - pTet August 25
MP172 L166 [http://partsregistry.org/Part:pSB1A2 pSB1A2] RBS(B0032) - tetR
Part icon rbs.pngIcon coding.png 		RBS(B0032) - tetR August 25

Digestion Products

(Legend: Vector, Insert) Ø

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Obs Size Matrix Size BB Description Product in use []ng/µL Date
D100.1 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 34 10 July 26
D100.2
D101 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2076 15 10 July 29
D102.1 [http://partsregistry.org/Part:BBa_B0034 B0034] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2077 14 10 July 26
August 15
D102.2
D103.1 [http://partsregistry.org/Part:BBa_J23101 J23101] [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV 2100 883 27 July 26
D103.2 35
D104.1 [http://partsregistry.org/Part:BBa_J23109 J23109] [http://partsregistry.org/Part:BBa_J61002 J61002] SpeI PstI BV 2100 883 20 July 26
D104.2
D105.1 [http://partsregistry.org/Part:BBa_R0079 R0079] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2222 14 5 July 26
D105.2
D106.1 [http://partsregistry.org/Part:BBa_R0040 R0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2119 14 8 July 26
D106.2
D107 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2750 14 August 23 5
12 		July 26
July 23
D108.1 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 707 5 July 29
D108.2
D109 [http://partsregistry.org/Part:BBa_S03154 S03154] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2056 708 July 26 10 July 26
D110 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2768 14 August 23 20
8
19 		July 26
August 23
August 28
D111.1 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 725 20 July 26
D111.2
D112 [http://partsregistry.org/Part:BBa_S03879 S03879] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2079 703 8 September 5
D113 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] EcoRI SpeI FI 4402 779 20 July 26
D114 [http://partsregistry.org/Part:BBa_C0079 C0079] [http://partsregistry.org/Part:pSB2K3 pSB2K3] XbaI PstI BI 4003 778 20 July 26
D115.1 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 4402 746 - July 29
D115.2
D116.1 [http://partsregistry.org/Part:BBa_C0179 C0179] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 745 20 July 29
D116.1
D117 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI SpeI FI 3166 746 5 July 29
D118 [http://partsregistry.org/Part:BBa_E0030 E0030] [http://partsregistry.org/Part:pSB2K3 pSB2K3] XbaI PstI BI 3167 745 20 July 26
D119 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI SpeI FI 2056 743 10 July 26
D120 [http://partsregistry.org/Part:BBa_E0040 E0040] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 742 10 July 26
D121 [http://partsregistry.org/Part:BBa_E1010 E1010] [http://partsregistry.org/Part:pSB2K3 pSB2K3] EcoRI SpeI FI 4402 704 10 July 26
D122 [http://partsregistry.org/Part:BBa_E1010 E1010] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 4403 703 10 July 26
D123.1 [http://partsregistry.org/Part:BBa_J23100 J23100] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 26 July 26
D123.2 23
D124.1 [http://partsregistry.org/Part:BBa_J23107 J23107] [http://partsregistry.org/Part:J61002 J61002] SpeI PstI BV 2100 883 15 July 26
D124.2
D125.1 [http://partsregistry.org/Part:BBa_B0015 B0015] [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] EcoRI XbaI FV 3303 15 26
10 		July 26
August 23
August 28
D125.2 24
3687
D126.1 [http://partsregistry.org/Part:BBa_I0500 I0500] [http://partsregistry.org/Part:pSB2K3 pSB2K3] SpeI PstI BV 5621 14 10 July 26
D126.2
D127.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 37 10 July 26
D127.2
D128.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] EcoRI XbaI FV 2079 15 10 July 29
D128.1
D129.1 [http://partsregistry.org/Part:BBa_B0030 B0030] [http://partsregistry.org/Part:pSB1A2 pSB1A2] SpeI PstI BV 2080 14 10 July 29
D129.2
D130.1 [http://partsregistry.org/Part:BBa_E0422 E0422] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 939 10 July 26
D130.2
D130.3
D131.1 [http://partsregistry.org/Part:BBa_E0840 E0840] [http://partsregistry.org/Part:pSB1A2 pSB1A2] XbaI PstI BI 2057 900 8 July 26
D131.2
D131.3
D132 pFlgA - EcoRI SpeI FI - 250 5 August 6
D133 pFlgB - EcoRI SpeI FI - 250 5 August 6
D134 pFlhB - EcoRI SpeI FI - 249 5 August 6
D135 pFlhDC - EcoRI SpeI FI - problem August 6
D136 - [http://partsregistry.org/Part:BBa_J61002 J61002] EcoRI SpeI FV 2925 - August 6
August 16
D137 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] EcoRI SpeI FV 2727 - August 6
D138 E0240 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 899 - August 6
D139 flhDC - EcoRI PstI FI - 972 August 6
D140 E040 rbs+ - PstI PstI I - August 12
D141 flhD RBS - - EcoRI SpeI FI - August 12
D142 flhC RBS - - EcoRI SpeI FI - August 12
D143 flhDC + promoter - EcoRI SpeI FI - August 12
D144 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] EcoRI SpeI FI - August 12
D145 pSB1A2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI - August 12
August 15
D146 MP148 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI 2057 1609 rbs TetR - GFP tripart
D110 (BV) - D131 (BI)
Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png 		August 15
D147 MP153 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 2056 799 Strongest RBS (1)- LasR activator (+LVA)
D102 (BV) - D114 (BI)
Part icon rbs.pngIcon coding.png 		August 15
D149 pfliL - EcoRI SpeI FI - August 14
August 16
D150 pfliL - XbaI PstI BI - August 14
August 16
D152 pSB3K3 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] XbaI PstI BI - August 14
D153 PCR 138 - gène flhDC - EcoRI SpeI FI - August 15
D154 PCR 140 - gène flhDC - EcoRI SpeI FI - August 15
D155 PCR 139 - pflhDC (URI) - EcoRI SpeI FI - August 15
D158 MP 155.1 - OmpR* [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI - August 15
D159 MP 156.1 - EnvZ* [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI - August 15
D161 MP 104 - pTet [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV - 39 August 16
D162 MP 114 - TetR [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI - 11 August 16
D163 MP 143 - gfp generator [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI - August 23 14
11 		August 16
August 23
D164 MP 101 - pJ23101 [http://partsregistry.org/Part:BBa_pJ61002 pJ61002] SpeI PstI BV - 3 August 16
D165 MP162.2 [http://partsregistry.org/Part:BBa_pSB1AK3 pSB1AK3] XbaI PstI BI 2057 798 rbs-lasR - double terminator
D147 (FI) - D125 (FV)
Part icon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png 		27 August 18
D166 MP 165.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV - 2942 bp RBS (B0034)+ YFP+ LVA- term B0015
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
59 		August 26
September 3
D179 MP3.4 [http://partsregistry.org/Part:BBa_pSB1AK3 pSB1AK3] SpeI PstI BV B0015 (double terminator B0010-B0012)
Part icon terminator.pngPart icon terminator.png 		August 20
D180 MP101.1 [http://partsregistry.org/Part:BBa_pJ61002 pJ61002] SpeI PstI BV promoter J23101
Part icon regulatory.png 		August 20
D181 MP104.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV pTet (TetR repressible promoter)
Part icon regulatory.png 		August 20
D182 MP114.1 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI TetR
Icon coding.png 		August 20
D183 MP119.3 - XbaI PstI BI pBad promoter
Part icon regulatory.png 		August 20
D184 MP143.1 [http://partsregistry.org/Part:BBa_pSB2K3 pSB2K3] EcoRI SpeI FI gfp generator
Part icon reporter.png 		August 18
D185 MP163.1 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV B0032 RBS
Part icon rbs.png 		August 18
D187 MP168.1 [http://partsregistry.org/Part:BBa_pSB1A3 pSB1A3] EcoRI XbaI FV RBS (B0034)+ mRFP LVA+ term B0015
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
43
September 3
D188 PCR 138 - XbaI PstI Insert - 767 bp OmpR*
Icon coding.png 		August 26
D189 MP 101.2 [http://partsregistry.org/Part:BBa_pJ61002 pJ61002] XbaI PstI Vector - 2073 bp pSB1A2
Icon plasmid.png 		August 26
D191 MP114.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV 2724 tetR
Icon coding.png 		22 August 29
D192 MP144.2 [http://partsregistry.org/Part:BBa_pJ61002 pJ61002] EcoRI XbaI FV 2267 FlgA
Part icon regulatory.png 		26 August 29
D193 MP171 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 961 gfp generator(E0240) - pTet
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.pngPart icon regulatory.png 		9,5 August 29
D194 MP172.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI SpeI FI 704 RBS(B0032) - tetR
Part icon rbs.pngIcon coding.png 		13 August 29
D195 MP172.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI 703 RBS(B0032) - tetR
Part icon rbs.pngIcon coding.png 		11 August 29
D196 MP104.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] SpeI PstI BV 2119 pTet
Part icon regulatory.png 		45 September 3
D197 MP157.2 [http://partsregistry.org/Part:BBa_pSB3K3 pSB3K3] XbaI PstI BI 898 gfp generator(E0240)
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png 		12 September 3
D198 MP164.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV 2981 RBS (B0034)+ ECFP+ LVA+ term B0015
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png 		36 September 3
D199 MP167.1 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV 2942 RBS (B0034)+ ECFP LVA- term (B0015)
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png 		61 September 3
D200 MP171.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] XbaI PstI BI 960 gfp generator(E0240) - pTet
Part icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.pngPart icon regulatory.png 		43 September 3
D201 MP114.2 [http://partsregistry.org/Part:BBa_pSB1A2 pSB1A2] EcoRI XbaI FV 2724 tetR
Icon coding.png 		13 August 29
D202 MP101.1 [http://partsregistry.org/Part:BBa_pJ61002 pJ61002] EcoRI nothing 2984 bp 25 September 8
D203 MP101.2 [http://partsregistry.org/Part:BBa_pJ61002 pJ61002] BspHI nothing 1870 bp
1008 bp
105 bp 		September 11

Strains (glycerol stock)

Strains glycerol stock
Number Name Biobricks Description Verified clone Date
S103 [http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3] Part icon regulatory.png AraC pBAD July 23
S106 [http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png ECFP (RBS+LVA+ Double Term) July 23
S107 [http://partsregistry.org/Part:BBa_B0034 B0034] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.png Strongest RBS (Efficiency = 1) July 23
S108 [http://partsregistry.org/Part:BBa_R0079 R0079] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.png promoter pLas (lasr & PAI regulated) July 23
S109 [http://partsregistry.org/Part:BBa_J23101 J23101] in [http://partsregistry.org/Part:BBa_J61002 pJ61002 ] Part icon regulatory.png Strongest Constitutive promoter July 23
S110 [http://partsregistry.org/Part:BBa_J23109 J23109] in [http://partsregistry.org/Part:BBa_J61002 pJ61002 ] Part icon regulatory.png Weak Constitutive promoter July 23
S111 [http://partsregistry.org/Part:BBa_R0040 R0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.png tetR repressible promoter July 23
S112 [http://partsregistry.org/Part:BBa_S03154 S03154] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.png B0034(rbs) ->LasI July 23
S113 [http://partsregistry.org/Part:BBa_S03879 S03879] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.png B0034(rbs) -> TetR July 23
S114 [http://partsregistry.org/Part:BBa_C0079 C0079] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] Icon coding.png lasR activator with LVA Tag July 23
S115 [http://partsregistry.org/Part:BBa_C0179 C0179] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png lasR activator July 23
S116 [http://partsregistry.org/Part:BBa_E0030 E0030] in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Part icon reporter.png YFP July 23
S117 [http://partsregistry.org/Part:BBa_E0040 E0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon reporter.png GFP July 23
S118 [http://partsregistry.org/Part:BBa_E1010 E1010] in [http://partsregistry.org/Part:pSB2K3 pSB2K3] Part icon reporter.png mRFP July 23
S119 OmpR M57I Icon coding.png July 23
S120 EnvZ I281S Icon coding.png July 23
S121 [http://partsregistry.org/Part:BBa_C0040 C0040] in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png tetR repressor July 23
S122 [http://partsregistry.org/Part:pSB1A3 pSB1A3] Icon plasmid.png July 23
S123 L102 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.png Strong rbs - YFP
D129 (BV) - D118 (BI) 		Aug 1
S124 L103 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.png Strong rbs - mRFP
D129 (BV) - D122 (BI) 		Aug 1
S125 L104 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.png Strong rbs - lasR activator
D129 (BV) - D114 (BI) 		Aug 1
S126 L105 in [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Strong promoter - ECFP
D123 (BV) - D130 (BI) 		Aug 1
S127 L106 in [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Strong promoter - gfp Tripart
D123 (BV) - D131 (BI) 		Aug 1
S128 L107 in [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Strongest promoter - ECFP
D103 (BV) - D130 (BI) 		Aug 1
S129 L108 in [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Strong promoter - GFP tripart
D103 (BV) - D131 (BI) 		Aug 1
S130 L110 in [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Medium promoter - gfp tripart
D124 (BV) - D131 (BI) 		Aug 1
S131 L111 in [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Weak promoter - ECFP
D104 (BV) - D130 (BI) 		Aug 1
S132 L112 in [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png Weak promoter - gfp Tripart
D104 (BV) - D131 (BI) 		Aug 1
S133 L115 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon reporter.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png pLas - ECFP
D105 (BV) - D130 (BI) 		Aug 1
S134 L116 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon reporter.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png pLas - gfp Tripart
D105 (BV) - D131 (BI) 		Aug 1
S135 L117 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Part icon reporter.pngPart icon terminator.pngPart icon terminator.png yfp - Double Terminator
D125 (FV) - D117 (FI) 		Aug 1
S136 L118 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Part icon reporter.pngPart icon terminator.pngPart icon terminator.png rfp - Double Terminator
D125 (FV) - D121 (FI) 		Aug 1
S137 L119 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Icon coding.pngPart icon terminator.pngPart icon terminator.png LasR activator + LVA - Double terminator
D125 (FV) - D113 (FI) 		Aug 1
S138 L121 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png pTet - gfp Tripart
D106 (BV) - D131 (BI) 		Aug 1
S139 L124 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.png Strongest rbs - mRFP
D102 (BV) - D122 (BI) 		Aug 1
S140 L125 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.png Strongest rbs - yfp
D102 (BV) - D118 (BI) 		Aug 1
S141 MG1655 Aug 7
S142 [http://partsregistry.org/Part:J61002 J61002] Icon plasmid.png Aug 7
S143 L128 in [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon reporter.png pFlgA-RFP
D132 (FI) - D136 (FV) 		MP144.3 ; MP144.4 August 12
S144 L129 in [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon reporter.png pFlgB-RFP
D133 (FI) - D136 (FV) 		MP145.6 ; MP145.7 August 12
S145 L130 in [http://partsregistry.org/Part:J61002 J61002] Part icon regulatory.pngPart icon reporter.png pFlhB-RFP
D134 (FI) - D136 (FV) 		MP146.1 ; MP146.2 August 12
S146 L100 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png rbs TetR - ECFP
D110 (BV) - D130 (BI) 		August 13
S147 L101 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png rbs TetR - GFP tripart
D110 (BV) - D131 (BI) 		August 13
S148 L114 in [http://partsregistry.org/Part:pSB2K3 pSB2K3] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png AracpBAD - gfp tripart
D126 (BV) - D131 (BI) 		August 13
S149 L120 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon regulatory.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png tetR repressible promoter - ECFP
D106 (BV) - D130 (BI) 		August 13
S150 L122 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png RBS-lasI - ECFP
D107 (BV) - D130 (BI) 		August 13
S151 L123 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.pngPart icon rbs.pngPart icon reporter.pngPart icon terminator.pngPart icon terminator.png RBS lasI - gfp tripart
D107 (BV) - D131 (BI) 		August 13
S152 L126 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Part icon rbs.pngIcon coding.png Strongest RBS (1)- LasR activator (+LVA)
D102 (BV) - D114 (BI) 		August 13
S153 L132 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png flhDC
D142(FV) - D139(FI) 		August 13
S154 L133 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png OmpR*
D142(FV) - D140(FI) 		August 13
S154 L133.1 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png OmpR*
D142(FV) - D140(FI) 		WT sequence August 19
S155 L134 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png EnvZ*
D142(FV) - D141(FI) 		August 13
S155 L134.2 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png EnvZ*
D142(FV) - D141(FI) 		lacks about 300 bp in EnvZ* August 19
S156 L138 in [http://partsregistry.org/Part:pSB3K3 pSB3K3] Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png gfp generator (E0240)
D137(FV) - D138(FI) 		August 13
S157 E0240 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png GFP generator August 15
S158 pSB3K3 Icon plasmid.png plasmid August 15
S159.1 L139.1 in in [http://partsregistry.org/Part:pSB3K3 pSB3K3] Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png E0240 RBS+
D140 (PstI) - D144 (ES) 		seems to have the plasmid without the insert and one with the insert August 15
S159.2 incorrect clone
S160.1 L141.1 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon coding.png flhD
D142 (ES) - D145 (ES) 		has not been sequenced August 15
S160.2 has not been sequenced
S160.3 has not been sequenced
S161.1 L142.7 in [http://partsregistry.org/Part:pSB1A2 pSB1A2] Icon regulatory.png flhDC+promotor
D143 (ES) - D145 (ES) 		does not seem to have the insert August 15
S161.2 incorrect clone
S162 MP162.1 in [http://partsregistry.org/Part:pSB1AK3 pSB1AK3] Icon rbs.pngIcon coding.pngPart icon terminator.pngPart icon terminator.png rbs-lasR + double terminator
D147 (ES) - D125 (EX) 		August 18
S163 B0032 in pSB1A2 Icon rbs.png medium rbs (efficiency = 0,3) August 20
S164 E0422 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png strongest rbs ECFP LVA+ double terminator August 20
S165 E0430 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png strongest rbs YFP LVA- double terminator August 20
S166 E0432 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png strongest rbs YFP LVA+ double terminator August 20
S167 E0420 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png
strongest rbs ECFP LVA- double terminator August 20
S168 I732078 Icon rbs.pngIcon reporter.pngPart icon terminator.pngPart icon terminator.png strongest rbs mRFP LVA+ double terminator August 20
Retrieved from "http://2008.igem.org/Team:Paris/Notebook/Freezer"