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Team:Chiba/jk/β/week 4
From 2008.igem.org
(Difference between revisions)
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===7 September, 2008=== | ===7 September, 2008=== | ||
-->(6/9)'''[[Team:Chiba/protocol/gelcheck|Gel Check]]''' | -->(6/9)'''[[Team:Chiba/protocol/gelcheck|Gel Check]]''' | ||
| + | Sample DNA : R0010 | ||
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | :<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
<tr> | <tr> | ||
| Line 22: | Line 23: | ||
<td>6</td></tr> | <td>6</td></tr> | ||
</table> | </table> | ||
| - | : | + | |
| - | :1 R0010 | + | : |
| - | :2 S03154 | + | |
| - | :3 J04500 | + | [[Team:Chiba/protocol/digestion|Digestion Test]] |
| - | :4 C0161 | + | :Sample DNA : |
| - | :5 C0261 | + | :1 R0010 |
| + | :2 S03154 | ||
| + | :3 J04500 | ||
| + | :4 C0161 | ||
| + | :5 C0261 | ||
| + | <table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
| + | <tr> | ||
| + | <td width="257">Sample No</td> | ||
| + | <td>1</td><td>2</td><td>3</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Sample DNA</td> | ||
| + | <td>1</td><td>1</td><td>1</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>PstⅠ</td> | ||
| + | <td>-</td><td>-</td><td>0.5</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>EcoRⅠ</td> | ||
| + | <td>-</td><td>0.5</td><td>0.5</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>Buffer 3</td> | ||
| + | <td>-</td><td>1</td><td>1</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>BSA</td> | ||
| + | <td>-</td><td>-</td><td>1</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>dH2O</td> | ||
| + | <td>-</td><td>7.5</td><td>2</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>TOTAL</td> | ||
| + | <td>1</td><td>10</td><td>10</td> | ||
| + | </tr> | ||
| + | </table> | ||
===8 September, 2008=== | ===8 September, 2008=== | ||
Revision as of 08:12, 30 September 2008
>送受信班
Contents |
Week 4
>last week
7 September, 2008
-->(6/9)Gel Check Sample DNA : R0010
Sample DNA 1 Loading Dye 1 dH2O 4 TOTAL 6 - Sample DNA :
- 1 R0010
- 2 S03154
- 3 J04500
- 4 C0161
- 5 C0261
Sample No 1 2 3 Sample DNA 1 1 1 PstⅠ - - 0.5 EcoRⅠ - 0.5 0.5 Buffer 3 - 1 1 BSA - - 1 dH2O - 7.5 2 TOTAL 1 10 10 8 September, 2008
9 September, 2008
10 September, 2008
11 September, 2008
12 September, 2008
13 September, 2008
- Gel Check
- BBa_C0161[http://partsregistry.org/Part:BBa_Co161](2007)
- BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)
- BBa_R0010[http://partsregistry.org/Part:BBa_R0010](2007)
Sample No 1~3 Sample DNA 1 Loading Dye 1 dH2O 4 TOTAL 6 - From left;
- From left;
-->Digestion
- BBa_C0161[http://partsregistry.org/Part:BBa_Co161](2007)
- BBa_J04500[http://partsregistry.org/Part:BBa_J04500](2007)
- BBa_R0010[http://partsregistry.org/Part:BBa_R0010](2007)
Sample No ① ② ③ ④ Sample DNA 5 5 1 1 EcoRⅠ 0.5 o.5 - - SpeⅠ 0.5 0.5 - - Buffer 1 1 1 - - Buffer 3 - - 1 - BSA 1 1 - - dH2O 2 2 7.5 - TOTAL 10 10 10 1 >next week
>next week
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