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Team:Hawaii/Notebook/2008-09-30
From 2008.igem.org
(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Plasmid Prep=== :<strong> Grace</strong> :* Plasmid prep of BBpRL1383a #1, 3, 13, 18 ===Construction of secretion dev...) |
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:::* rbs+GFPf+tt #1, 2 | :::* rbs+GFPf+tt #1, 2 | ||
:::* GFPf+tt #21 | :::* GFPf+tt #21 | ||
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| + | ===Plasmid Prep=== | ||
| + | :<strong> Margaret</strong> | ||
| + | |||
| + | :*pSB1A3, plac/rbs/rep + pSB1A3 (also made a glycerol stock of this), and oriT in pSB1A3 | ||
| + | |||
| + | ===Re-digest from yesterday=== | ||
| + | :<strong> Margaret </strong> | ||
| + | [[Image:gel_purification_9-30.jpg|right|thumb|300px|Gel purification of re-digest of aadaBB, aadapRL, and oriV.]] | ||
| + | :* gel purification of aadaBB, aadApRL, and oriV | ||
| + | :* de-phosphorylate pSB1A2, pSB1A3a, pSB3K3 | ||
| + | |||
= Discussion = | = Discussion = | ||
Revision as of 21:41, 30 September 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Plasmid Prep
- Grace
- Plasmid prep of BBpRL1383a #1, 3, 13, 18
Construction of secretion device (cont.)
- Grace
- PCR:
- nir+rbs
- slr1+GFPf
- plac+rbs
- rbs+GFP
- nir
- nir+rbs+pilA #17 (yet to be confirmed via sequencing)
- rbs+GFPf+tt #1, 2 (yet to be confirmed via sequencing)
- GFPf+tt #21
- RE digest:
- EcoRI/PstI:
- pSB1A3
- EcoRI/SpeI:
- nir
- rbs+GFP
- nir+rbs
- plac+rbs
- nir+rbs+pilA #17
- XbaI/PstI:
- slr1+GFPf
- rbs+GFPf+tt #1, 2
- GFPf+tt #21
Plasmid Prep
- Margaret
- pSB1A3, plac/rbs/rep + pSB1A3 (also made a glycerol stock of this), and oriT in pSB1A3
Re-digest from yesterday
- Margaret
File:Gel purification 9-30.jpg
Gel purification of re-digest of aadaBB, aadapRL, and oriV.
- gel purification of aadaBB, aadApRL, and oriV
- de-phosphorylate pSB1A2, pSB1A3a, pSB3K3
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
