Team:KULeuven/22 August 2008

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== Lab Work ==
== Lab Work ==
=== Wet Lab ===
=== Wet Lab ===
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* We made digests (with ''Eco''RI and ''Spe''I) of the following ligations: R0084+J23022+J23109+J23032, R0084+B0032, R0040+J23022 and K145001. After that, these digests were put on gel. They seemed to have been cut properly, but when we purified these parts from the gel the concentrations were very low.
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[[image:gel-22-8.jpg|left|thumb|350px|Gel with the PCR products of the transduction, apparently we used to much template]]
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* We also did some PCRs to construct BioBrick K145014 (T7 polymerase with UmuD tag) and to test the transduction.
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* We started a few ligations (the ones that failed before): C0040+B0015, K145015+B0015, C0012+B0015, C0060+B0015, K145001+B0015 and R0040+J23022.
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We made digests (with ''Eco''RI and ''Spe''I) of the following ligations: [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022], [http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_B0032 B0032], [http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032] and [http://partsregistry.org/Part:BBa_K145001 K145001]. After that, these digests were put on gel. They seemed to have been cut properly, but when we purified these parts from the gel the concentrations were very low.
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* We MiniPrepped K145151 + B0015.
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We also did some PCRs to construct BioBrick [http://partsregistry.org/Part:BBa_K145014 K145014] (T7 polymerase with UmuD tag) and to test the transduction, both PCRs failed. The PCR to test the transduction was set up again, now with less template.
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We started a few ligations (the ones that failed before): [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:BBa_B0015 B0015] and [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23032 J23032].
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We miniprepped [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015].
=== Dry Lab ===
=== Dry Lab ===
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We had a very good meeting today, decided upon some things. For instance that [http://partsregistry.org/Part:BBa_M30109 M30109], the light receptor, goes.
==== Modeling ====
==== Modeling ====
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Stochastic modeling and cutting the big model up into it's parts for re-testing :)
==== Wiki ====
==== Wiki ====
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Tippenvulwedstrijd:
Tippenvulwedstrijd:
Hanne: 1 min 29!
Hanne: 1 min 29!
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Getting better!  
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Getting better!
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Latest revision as of 17:17, 11 October 2008

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Contents

Lab Work

Wet Lab

Gel with the PCR products of the transduction, apparently we used to much template

We made digests (with EcoRI and SpeI) of the following ligations: [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23022 J23022], [http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_B0032 B0032], [http://partsregistry.org/Part:BBa_R0084 R0084]+[http://partsregistry.org/Part:BBa_J23022 J23022]+[http://partsregistry.org/Part:BBa_J23109 J23109]+[http://partsregistry.org/Part:BBa_J23032 J23032] and [http://partsregistry.org/Part:BBa_K145001 K145001]. After that, these digests were put on gel. They seemed to have been cut properly, but when we purified these parts from the gel the concentrations were very low.

We also did some PCRs to construct BioBrick [http://partsregistry.org/Part:BBa_K145014 K145014] (T7 polymerase with UmuD tag) and to test the transduction, both PCRs failed. The PCR to test the transduction was set up again, now with less template.

We started a few ligations (the ones that failed before): [http://partsregistry.org/Part:BBa_C0060 C0060]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0040 C0040]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_C0012 C0012]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145015 K145015]+[http://partsregistry.org/Part:BBa_B0015 B0015], [http://partsregistry.org/Part:BBa_K145001 K145001]+[http://partsregistry.org/Part:BBa_B0015 B0015] and [http://partsregistry.org/Part:BBa_R0040 R0040]+[http://partsregistry.org/Part:BBa_J23032 J23032].

We miniprepped [http://partsregistry.org/Part:BBa_K145151 K145151]+[http://partsregistry.org/Part:BBa_B0015 B0015].

Dry Lab

We had a very good meeting today, decided upon some things. For instance that [http://partsregistry.org/Part:BBa_M30109 M30109], the light receptor, goes.

Modeling

Stochastic modeling and cutting the big model up into it's parts for re-testing :)

Wiki

Official super duper release of wiki homepage and global style, but needs fixes to match with annoying IE browser. Picture gallery fixes will have to wait.

Remarks

Tippenvulwedstrijd: Hanne: 1 min 29! Getting better!

Retrieved from "http://2008.igem.org/Team:KULeuven/22_August_2008"