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Alberta NINT/11 July 2008
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[[Team:Alberta_NINT/Notebook | < Back to notebook]] | [[Team:Alberta_NINT/Notebook | < Back to notebook]] | ||
| - | [[Alberta_NINT/10_July_2008 | < Previous entry]] | + | [[Alberta_NINT/10_July_2008 | < Previous entry]] | [[Alberta_NINT/14_July_2008 | Next entry >]] |
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Lac Z Activity Assay was unsuccessful - perhaps not enough cells were used? | Lac Z Activity Assay was unsuccessful - perhaps not enough cells were used? | ||
| - | + | lab work (JD): | |
| + | Carried out Day 2 protocol for Activity Assay | ||
| + | Like stated above, was not successful. | ||
| + | Collected quantitative data of restriction fragments for ligation | ||
| + | Ligated K102003+pSB4A5=K102031 and K102006+pSB4A5=K102021 | ||
| + | Transformed XL1-B Cells | ||
| + | Plated cells on LB + Amp50 | ||
Latest revision as of 22:59, 11 October 2008
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lab work (SD):
Measured cell count using spectrophotometer of 10/07/08 overnights.
Lac Z Activity Assay was unsuccessful - perhaps not enough cells were used?
lab work (JD):
Carried out Day 2 protocol for Activity Assay
Like stated above, was not successful.
Collected quantitative data of restriction fragments for ligation
Ligated K102003+pSB4A5=K102031 and K102006+pSB4A5=K102021
Transformed XL1-B Cells
Plated cells on LB + Amp50
