Team:Chiba/Calendar-Home/7 September 2008

From 2008.igem.org

(Difference between revisions)

Revision as of 15:45, 24 October 2008

>Home | Notebook

6 September 2008 <|> 8 September 2008

Contents

1 Laboratory work

Laboratory work

Team:Input

no work

Team:Communication

-->(6/9) Digestion Test

Sample DNA 1 : BBa_R0010(unknown)[http://partsregistry.org/Part:BBa_R0010]

Sample DNA 2 : BBa_R0010(100ng/μL)[http://partsregistry.org/Part:BBa_R0010]

Sample No	1,2
Sample DNA	12
PstⅠ	0.5
SpeⅠ	0.5
Buffer 2	2
(×10)BSA	2
dH₂O	3
TOTAL	20

--->Gel Check

Sample	left	right
Sample DNA	5	5
Loading Dye	1	1
TOTAL	6	6

Left side:BBa_R0010 Sample No.1

Right side:BBa_R0010 Sample No.2

-->(8/9)

Team:Output

Cloning Digestion

vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]① 75ng/μl
insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158]② 33.6ng/μl
insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156]③ 33.6ng/μl
insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160]④ 38.4ng/μl
vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]⑤ 100ng/μl
insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]⑥ 33.6ng/μl

Sample No.	①	②	③	④	⑤	⑥
DNA(digested sample)	50	54	54	45	14	54
SpeⅠ	1	0	0	0	1	0
PstⅠ	0.5	0.5	0.5	0.5	0.5	0.5
XbaⅠ	0	0.5	0.5	0.5	0	0.5
BSA(x10)	0.6	7	7	6	2	7
Buffer2	6	-	-	-	2	-
Buffer3	-	7	7	6	-	7
dH₂O	1.9	1	1	2	0.5	1
TOTAL	60μl	70μl	70μl	60μl	20μl	70μl

-->37℃ 3hour

-->Gel Extraction

Sample No.	①	②	③	④	⑤	⑥
DNA(digested sample)	6	10	10	10	4	10
Loading Dye	2	2	2	2	1	2
dH₂O	4	-	-	-	1	2
TOTAL	12μl	12μl	12μl	12μl	6μl	12μl
well	10	7	7	6	5	7

-->DNA purification (①-④,⑥:7μl ⑤:6μl)

Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/7_September_2008"